Sequence diversity in the surface-exposed amino-terminal region of the coat proteins of seven strains of sugarcane mosaic virus correlates with their host range

Xiao, X. W.; Frenkel, M. J.; Teakle, D. S.; Ward, Colin W.; Shukla, D. D.

doi:10.1007/bf01309548

Cited by 49 publications

(26 citation statements)

References 16 publications

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“…Our results confirm that between isolates of PRSV, the core is also conserved and the N terminus is most variable. Xiao et al (1993) suggested that the N terminus may be involved in host specificity. However, we have found no obvious sequence changes, either nucleotide or amino acid, that would account for the difference between PRSV-P and PRSV-W. Interestingly, the C terminus of the coat protein was highly conserved between all PRSV isolates.…”

Section: Discussionmentioning

confidence: 99%

Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

Bateson

Chaleeprom

et al. 1994

Journal of General Virology

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We have sequenced the coat protein gene of nine isolates of papaya ringspot virus (PRSV) including six Australian and three Asian isolates and compared these with four previously reported sequences of PRSV. There was up to 12 % sequence variation between isolates at the nucleotide level. However, there was no significant difference between the sequences obtained from Australian isolates irrespective of whether they were PRSV type P (cucurbit or papaya infecting) or PRSV type W (cucurbit infecting) and these isolates were more closely related to one another than to any other isolate. These results imply that PRSV-P, first recorded in Australia in 1991, arose locally from PRSV-W (first recorded in Australia in 1978) rather than being introduced. Further, there was no consistent sequence difference between PRSV-P and PRSV-W isolates that would obviously account for their host range difference.

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Section: Discussionmentioning

confidence: 99%

Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

Bateson

Chaleeprom

et al. 1994

Journal of General Virology

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“…Sugarcane mosaic virus (SCMV) causes severe dwarf mosaic disease in maize (Zea mays), sugarcane (Saccharum sinensis), and sorghum (Sorghum vulgare; Xiao et al, 1993). It is known as a major viral pathogen of maize in China, and the Beijing isolate (SCMV-BJ) belongs to a prevalent strain of SCMV in China (Fan et al, 2003).…”

mentioning

confidence: 99%

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

et al. 2017

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RNA silencing plays a critical role against viral infection. To counteract this antiviral silencing, viruses have evolved various RNA silencing suppressors. Meanwhile, plants have evolved counter-counter defense strategies against RNA silencing suppression (RSS). In this study, the violaxanthin deepoxidase protein of maize (Zea mays), ZmVDE, was shown to interact specifically with the helper component-proteinase (HC-Pro; a viral RNA silencing suppressor) of Sugarcane mosaic virus (SCMV) via its mature protein region by yeast two-hybrid assay, which was confirmed by coimmunoprecipitation in Nicotiana benthamiana cells. It was demonstrated that amino acids 101 to 460 in HC-Pro and the amino acid glutamine-292 in ZmVDE mature protein were essential for this interaction. The mRNA levels of ZmVDE were down-regulated 75% to 65% at an early stage of SCMV infection. Interestingly, ZmVDE, which normally localized in the chloroplasts and cytoplasm, could relocalize to HC-Pro-containing aggregate bodies in the presence of HC-Pro alone or SCMV infection. In addition, ZmVDE could attenuate the RSS activity of HC-Pro in a specific protein interaction-dependent manner. Subsequently, transient silencing of the ZmVDE gene facilitated SCMV RNA and coat protein accumulation. Taken together, our results suggest that ZmVDE interacts with SCMV HC-Pro and attenuates its RSS activity, contributing to decreased SCMV accumulation. This study demonstrates that a host factor can be involved in secondary defense responses against viral infection in monocot plants.

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“…The N-terminal coat protein region of AbaMV had amino acid repeats (Fig. 3) which differed from those published for other SCMV isolates, including SCMV-MDB, SCMV-SC and SCMV-Sabi (Xiao et al 1993;Ward et al 1995). There were no amino acid repeats detected in BBrMV that were similar to those in AbaMV.…”

Section: Sequence Analysesmentioning

confidence: 73%

“…It has been suggested that host specificity and virulence are determined by the coat protein, especially the N-terminal region, which varies in size and similarity between the SCMV subgroup members (Xiao et al 1993). However, the N-terminal repeat sequences described for the maize-infecting SCMV-MDB (Xiao et al 1993) are not present in maize-infecting isolates of SCMV from Germany (Oertel et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Abacá mosaic virus: a distinct strain ofSugarcane mosaic virus

Gambley

Thomas

Magnaye

et al. 2004

Austral. Plant Pathol.

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Abstract. Abacá mosaic virus (AbaMV) is related to members of the sugarcane mosaic virus subgroup of the genus Potyvirus. The ∼2 kb 3 terminal region of the viral genome was sequenced and, in all areas analysed, found to be most similar to Sugarcane mosaic virus (SCMV) and distinct from Johnsongrass mosaic virus (JGMV), Maize dwarf mosaic virus (MDMV) and Sorghum mosaic virus (SrMV). Cladograms of the 3 terminal region of the NIb protein, the coat protein core and the 3 untranslated region showed that AbaMV clustered with SCMV, which was a distinct clade and separate from JGMV, MDMV and SrMV. The N-terminal region of the AbaMV coat protein had a unique amino acid repeat motif different from those previously published for other strains of SCMV. The first experimental transmission of AbaMV from abacá (Musa textilis) to banana (Musa sp.), using the aphid vectors Rhopalosiphum maidis and Aphis gossypii, is reported. Polyclonal antisera for the detection of AbaMV in western blot assays and ELISA were prepared from recombinant coat protein expressed in E. coli. A reverse transcriptase PCR diagnostic assay, with microtitre plate colourimetric detection, was developed to discriminate between AbaMV and Banana bract mosaic virus, another Musa-infecting potyvirus. Sequence data, host reactions and serological relationships indicate that AbaMV should be considered a distinct strain of SCMV, and the strain designation SCMV-Ab is suggested.

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Sequence diversity in the surface-exposed amino-terminal region of the coat proteins of seven strains of sugarcane mosaic virus correlates with their host range

Cited by 49 publications

References 16 publications

Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

Abacá mosaic virus: a distinct strain ofSugarcane mosaic virus

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