Sequence Diversity in the NSm Gene of Groundnut Bud Necrosis Virus Isolates Originating from Different Hosts and Locations in India

Abstract

The movement protein (NSm) gene of Groundnut bud necrosis virus (GBNV) isolates from pea, mungbean, cowpea, French bean, tomato and potato collected from different locations of India were compared to study their diversity. The NSm gene sequences of all the GBNV isolates were highly conserved and had only 0–3% diversity in amino acids and 0–10% in nucleotides. Comparison of amino acid sequence of NSm gene of 25 GBNV isolates revealed the presence of many conserved regions. Both ‘D‐motif’ and ‘G‐residue’, the conserved regions of ‘30K superfamily’ of virus movement protein, were present in all the isolates. Clustering of the GBNV isolates does not appear to be based on their place of origin and host plant species.

Diseases of Edible Oilseed Crops

“…Though the detailed study in strain differentiation has not been done, it is revealed through the nucleotide and amino acid sequences of the movement protein (NSm) genes of different isolates that the NSm genes of PBNV isolates are identical in length (924 bp encoding 307 amino acids) suggest ing their common origin (Akram et al 2003(Akram et al , 2004(Akram et al , 2012. The primary sources of inoculum may be different host plant members of Leguminosae and Solanaceae specially crop plants such as tomato, mung bean, urd bean, sunflower, and cucumber that are cultivated in summer and weed hosts such as Ageratum conyzoides, Acanthospermum hispidum, and Cassia tora, which are more commonly present in and around the peanut crop fields and which sustain virus infection and effective thrips vector population (Nagaraja et al 2005a, Gopal et al 2011a).…”