Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomal RNA among three <i>Moniezia</i> species isolated from ruminants in Japan

Abstract

This study was designed to clarify the differences in the internal transcribed spacer (ITS) 1 and 5.8S nucleotide sequences of Moniezia expansa, M. benedeni and M. monardi isolated from ruminants in Japan and to determine their phylogenetic relationships. A 98% similarity in the 5.8S sequences was observed among the 3 Moniezia species, whereas many nucleotide indels and substitutions were observed in the ITS1 sequences among the three Moniezia species. These results suggest that the ITS1 region could serve as a potential marker for discriminating the 3 Moniezia species. In the phylogenetic tree based on the ITS1 sequences, M. monardi and M. benedeni showed genetically closer relationship to each other than to M. expansa.

Molecular Identification and Phylogenetic-Tree Analysis of Moniezia Species From Sheep in Al-Diwaniyah City

“…Monieziasis is the term of illness that is caused by species of Moniezia, for this genus as a tapeworm has limited species such as M. expansa (Rudolphi, 1810), M. benedeni (Moniez 1879) and M. monarda (Ohtori et al, 2015).M. expansa affects sheep (high incidence), cattle, goats, swine, and very rarely human (El-Shazly et al, 2004;Gómez-Puerta, 2008).…”

Genetic characterization of Moniezia species in Senegal and Ethiopia

“…Although at least seven species have been described [12], morphological identification of the species is not always easy. Besides, genetic information is available for only three species of the genus, Moniezia expansa, Moniezia benedeni and Moniezia monardi [13]. Morphologically, M. expansa and M. benedeni are distinguishable based on their interproglottidal glands (IPGs) [12].…”

Molecular characterization of Moniezia sichuanensis in captive musk deer (Moschus berezovskii)

“…In recent years, molecular methods have been introduced that help the classification and determination of Moniezia spp., with cytochrome c oxidase subunit 1 (COX1) and ITS genes being the most frequently used markers (Nguyen et al , 2012; Diop et al , 2015; Ohtori et al , 2015). ITS could serve as a good genetic marker because it can differentiate M. expansa and M. benedeni recovered from cattle, goats and sheep (Nguyen et al , 2012).…”

“…ITS could serve as a good genetic marker because it can differentiate M. expansa and M. benedeni recovered from cattle, goats and sheep (Nguyen et al , 2012). In addition, ITS can discriminate M. monardi from Japanese serows, M. benedeni from cattle and M. expansa from sheep (Ohtori et al , 2015). Due to sequence variation caused by the rapid evolution of ITS genes, this gene could contribute to our understanding of the intra- and interspecific phylogenetic relationships among Moniezia spp., as well as the identification of a new species at the molecular level (Bowles et al , 1995; Fazaeli et al , 2000).…”

“…However, genetic information for the genus Moniezia is of limited availability, making it difficult to explore the molecular characteristics of this species. Genetic information has been reported recently for M. expansa , M. benedeni and M. monardi (Ohtori et al , 2015), as well as two undefined Moniezia species (Wickström et al ., 2005), making it possible to study M. sichuanensis .…”