Sequence determination of a satellite RNA isolated from Aspergillus foetidus

Shah, Unnati A; Kotta‐Loizou, Ioly; Coutts, Robert H.A.

doi:10.1007/s00705-015-2338-x

Cited by 5 publications

(4 citation statements)

References 18 publications

(14 reference statements)

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“…Kuroki Misa and colleagues recently deposited the nt sequences of a partitivirus showing up to eight dsRNA segments (NCBI accessions from LC763222 to LC763246 and from LC763268 to LC763275), identified from four different Aspergillus flavus isolates. Moreover, additional satellite RNA segments have been found in association with helper viruses in the Partitiviridae ( Shah, Kotta-Loizou, and Coutts 2015 ; Jiang et al 2022 ), with the former being capable of reducing both pathogenicity and genomic accumulation of the respective helper virus ( Jiang et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

A collection of Trichoderma isolates from natural environments in Sardinia reveals a complex virome that includes negative-sense fungal viruses with unprecedented genome organizations

et al. 2023

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Trichoderma genus includes soil-inhabiting fungi that provide important ecosystem services in their interaction with plants and other fungi, as well as biocontrol of fungal plant diseases. A collection of Trichoderma isolates from Sardinia has been previously characterized, but here we selected 113 isolates, representatives of the collection, and characterized their viral components. We carried out high-throughput sequencing of ribosome-depleted total RNA following a bioinformatics pipeline that detects virus-derived RNA-directed RNA polymerases (RdRps) and other conserved viral protein sequences. This pipeline detected seventeen viral RdRps with two of them corresponding to viruses already detected in other regions of the world and the remaining fifteen representing isolates of new putative virus species. Surprisingly, eight of them are from new negative-sense RNA viruses, a first in the genus Trichoderma. Among them is a cogu-like virus, closely related to plant-infecting viruses. Regarding the positive-sense viruses, we report the presence of an ‘ormycovirus’ belonging to a recently characterized group of bisegmented single-stranded RNA viruses with uncertain phylogenetic assignment. Finally, for the first time, we report a bisegmented member of Mononegavirales which infects fungi. The proteins encoded by the second genomic RNA of this virus were used to re-evaluate several viruses in the Penicillimonavirus and Plasmopamonavirus genera, here shown to be bisegmented and encoding a conserved polypeptide that has structural conservation with the nucleocapsid domain of rhabdoviruses.

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Section: Discussionmentioning

confidence: 99%

A collection of Trichoderma isolates from natural environments in Sardinia reveals a complex virome that includes negative-sense fungal viruses with unprecedented genome organizations

et al. 2023

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“…The ability of satellite RNAs to reduce the helper virus-mediated symptoms has generally coincided with a reduction in the level of accumulation of the helper virus in infected host cells ( Babos and Kassanis, 1962 ; Francki et al, 1986 ; Palukaitis, 2016 ). A similar satellite RNA (439 bp) has been found in A. foetidus ( Shah et al, 2015 ), but its biological functions are not well studied.…”

Section: Discussionmentioning

confidence: 82%

“…Recently, it has been reported that some mycoviruses additionally harbor satellite RNA segments ( Liu et al, 2015 ; Shah et al, 2015 ). Most satellite segments are classified as satellite viruses and satellite nucleic acids whether DNA or RNA ( Gnanasekaran and Chakraborty, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

A Satellite dsRNA Attenuates the Induction of Helper Virus-Mediated Symptoms in Aspergillus flavus

Jiang

Liu

et al. 2022

Front. Microbiol.

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Aspergillus flavus is an important fungal pathogen of animals and plants. Previously, we reported a novel partitivirus, Aspergillus flavus partitivirus 1 (AfPV1), infecting A. flavus. In this study, we obtained a small double-stranded (ds) RNA segment (734 bp), which is a satellite RNA of the helper virus, AfPV1. The presence of AfPV1 altered the colony morphology, decreased the number of conidiophores, created significantly larger vacuoles, and caused more sensitivity to osmotic, oxidative, and UV stresses in A. flavus, but the small RNA segment could attenuate the above symptoms caused by the helper virus AfPV1 in A. flavus. Moreover, AfPV1 infection reduced the pathogenicity of A. flavus in corn (Zea mays), honeycomb moth (Galleria mellonella), mice (Mus musculus), and the adhesion of conidia to host epithelial cells, and increased conidial death by macrophages. However, the small RNA segment could also attenuate the above symptoms caused by the helper virus AfPV1 in A. flavus, perhaps by reducing the genomic accumulation of the helper virus AfPV1 in A. flavus. We used this model to investigate transcriptional genes regulated by AfPV1 and the small RNA segment in A. flavus, and their role in generating different phenotypes. We found that the pathways of the genes regulated by AfPV1 in its host were similar to those of retroviral viruses. Therefore, some pathways may be of benefit to non-retroviral viral integration or endogenization into the genomes of its host. Moreover, some potential antiviral substances were also found in A. flavus using this system.

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“…The second dsRNA element is provisionally designated Aspergillus foetidus Exartavirus (AfExV; exarta- = “dependent” (for encapsidation) in Greek), contains an ORF encoding an RdRP, 100 kDa in size, and an unusually long 5′-UTR (Kozlakidis et al, 2013b ). The smallest dsRNA is a non-coding satellite RNA with a 5′-terminal sequence identical to that of AfExV (Shah et al, 2015 ) suggesting that both molecules are replicated by AfExV RdRP, while all three dsRNAs are encapsidated by the AfVV-1 CP.…”

Section: Molecular Characterisation Of the Aspergillus mentioning

confidence: 99%

Mycoviruses in Aspergilli: A Comprehensive Review

2017

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Fungi, similar to all species, are susceptible to viral infection. Aspergillus is arguably the most well studied fungal genus because of its medical, ecological and economical significance. Mycoviruses were initially detected in Aspergillus species almost 50 years ago and the field continues to be active today with ground-breaking discoveries. The aim of the present review is to cover the scientific progress in all aspects of mycovirology as exemplified by Aspergillus-focused research. Initially an overview of the population studies illustrating the presence of mycoviruses in numerous important Aspergillus species, such as A. niger, A. flavus, and A. fumigatus with be presented. Moreover the intricacies of mycovirus transmission, both inter- and intra-species, will be discussed together with the methodologies used to investigate viral dispersion in a laboratory setting. Subsequently, the genomic features of all molecularly characterized mycoviruses to date will be analyzed in depth. These include members of established viral families, such as Partitiviridae, Chrysoviridae and Totiviridae, but also more recent, novel discoveries that led to the proposal of new viral families, such as Polymycoviridae, Alternaviridae and, in the context of the present review, Exartaviridae. Finally, the major issue of phenotypic effects of mycoviral infection on the host is addressed, including aflatoxin production in A. flavus, together with growth and virulence in A. fumigatus. Although the molecular mechanisms behind these phenomena are yet to be elucidated, recent studies suggest that by implication, RNA silencing may be involved.

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Sequence determination of a satellite RNA isolated from Aspergillus foetidus

Cited by 5 publications

References 18 publications

A collection of Trichoderma isolates from natural environments in Sardinia reveals a complex virome that includes negative-sense fungal viruses with unprecedented genome organizations

A collection of Trichoderma isolates from natural environments in Sardinia reveals a complex virome that includes negative-sense fungal viruses with unprecedented genome organizations

A Satellite dsRNA Attenuates the Induction of Helper Virus-Mediated Symptoms in Aspergillus flavus

Mycoviruses in Aspergilli: A Comprehensive Review

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