Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1

McGeoch, Duncan J.; Dolan, Aidan; Donald, Sally; Rixon, Frazer J.

doi:10.1016/0022-2836(85)90320-1

Cited by 507 publications

(470 citation statements)

References 55 publications

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“…The DNA sequences of plasmid cloned copies of HSV-1 strain 17 restriction fragments were determined by the M13/chain terminator system as described (5,9,10). For the sequence reported in this paper, fragments used were EcoRI j cloned in pACYC184 (from V.G.…”

Section: Methodsmentioning

confidence: 99%

DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus

McGeoch

Davison

1986

Nucl Acids Res

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We have determined the sequence of herpes simplex virus type 1 DNA around the previously mapped location of sequences encoding an epitope of glycoprotein gH, and have deduced the structure of the gH gene and the amino acid sequence of gH. The unprocessed polypeptide is predicted to contain 838 amino acids, and to possess an N-terminal signal sequence and a C-terminal transmembrane sequence. Temperature-sensitive mutant tsQ26 maps within the predicted gH coding sequence. Homologous genes were identified in the genomes of two other herpesviruses, namely varicella-zoster virus and Epstein-Barr virus.

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Section: Methodsmentioning

confidence: 99%

DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus

McGeoch

Davison

1986

Nucl Acids Res

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“…The Us9 gene in HSV-1 encodes a 90 amino acid protein with a proposed molecular weight of 10,000 (McGeoch et al, 1985;Frame et al, 1986;Brandimarti and Roizman, 1997). The protein has multiple putative post-translational modification sites, e.g.…”

Section: Characterization Of the Us9 Null And Repaired Strainsmentioning

confidence: 99%

Viral regulation of the long distance axonal transport of herpes simplex virus nucleocapsid

et al. 2007

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Abstract-Many membranous organelles and protein complexes are normally transported anterograde within axons to the presynaptic terminal, and details of the motors, adaptors and cargoes have received significant attention. Much less is known about the transport in neurons of non-membrane bound particles, such as mRNAs and their associated proteins. We propose that herpes simplex virus type 1 (HSV) can be used to study the detailed mechanisms regulating long distance transport of particles in axons. A critical step in the transmission of HSV from one infected neuron to the next is the polarized anterograde axonal transport of viral DNA from the host infected nerve cell body to the axon terminal. Using the in vivo mouse retinal ganglion cell model infected with wild type virus or a mutant strain that lacks the protein Us9, we found that Us9 protein was necessary for long distance anterograde axonal transport of viral nucleocapsid (DNA surrounded by capsid proteins), but unnecessary for transport of virus envelope. Thus, we conclude that nucleocapsid can be transported independently down axons via a Us9-dependent mechanism.

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“…We have also speculated that the large extracellular domain(s) of gE/gI may also play a role in cellto-cell spread, perhaps by binding to proteins that are components of cell junctions, e.g., CAMs (22,55). This may explain the preferential localization of gE/gI to cell junctions (55,98), and there is evidence that gE, gI, and gD may have been derived from a common ancestor (52). By this scenario, gE/gI could act as a receptor-binding protein, much as gD, acting specifically at cell junctions to promote virus entry into recipient cells (Fig.…”

Section: Alphaherpesvirusesmentioning

confidence: 99%

Directed Egress of Animal Viruses Promotes Cell-to-Cell Spread

Johnson

Huber

2002

J Virol

229

189

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Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1

Cited by 507 publications

References 55 publications

DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus

DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus

Viral regulation of the long distance axonal transport of herpes simplex virus nucleocapsid

Directed Egress of Animal Viruses Promotes Cell-to-Cell Spread

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