Sequence determinants as key regulators in gene expression of T cells

Abstract

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The relationship of mRNA with protein expression in CD8+T cells associates with gene class and gene characteristics

“…UTR length alone, however, is unlikely to be the sole factor that influences mRNA and protein abundance. Similar to the increased presence of ARE clusters in longer 3’UTRs [26], the overall likelihood of containing regulatory motifs and structures increases with length. Notably, murine T cells activated for 48h with aCD3/CD28 beads display substantial shortening of 3’UTRs [46].…”

“…Untranslated regions of RNAs are critical components in defining the RNA levels and the protein output of a given mRNA, and the length of 5’UTR and 3’UTRs is contributing [26]. We therefore also compared the length of 5’UTRs and 3’UTRs across gene classes, and found that they show similar patterns (Fig 3B, C).…”

“…We therefore examined the sequence conservation of the different gene classes, by comparing the sequence homology at the gene level between human and zebrafish (Danio rerio). In particular the sequence of mammalian ribosomal proteins was highly conserved (median: 90.4% sequence homology), followed by TCA cycle genes with a median value of 75.47% sequence homology (Fig 3A Untranslated regions of RNAs are critical components in defining the RNA levels and the protein output of a given mRNA, and the length of 5'UTR and 3'UTRs is contributing [26]. We therefore Also the GC content can influence a gene's expression.…”

The relationship of mRNA with protein expression in CD8+ T cells associates with gene class and gene characteristics

“…Untranslated regions of RNAs are critical components in defining the RNA levels and the protein output of a given mRNA, and the length of 5’UTR and 3’UTRs is contributing [ 34 ]. We therefore also compared the length of 5’UTRs and 3’UTRs across gene classes, and we found that they show similar patterns ( Fig 3B and 3C ).…”

Impact of epigenetics on human health and possible tool for remediation

“…This impacts development of diseased states like cancer, allergy, inflammation, neurological disorders, maintenance of cellular homeostasis by autophagy, etc. [3,7,9,10,37]. In the present collection, Ghosh Roy has discussed about how wide range of diseases like cardiovascular, renal and gastrointestinal disorders to cancers like glioblastoma and neurodegenerative diseases; related to autophagy can be understood in the light of miRNA [47].…”