Sequence-dependent electrical response of ssDNA-decorated carbon nanotube, field-effect transistors to dopamine

Abstract

SummarySingle-walled carbon nanotube (SWCNT)-based field-effect transistors (FETs) have been explored for use as biological/chemical sensors. Dopamine (DA) is a biomolecule with great clinical significance for disease diagnosis, however, SWCNT FETs lack responsivity and selectivity for its detection due to the presence of interfering compounds such as uric acid (UA). Surface modification of CNTs using single-stranded deoxyribonucleic acid (ssDNA) renders the surface responsive to DA and screens the interferent. Due to the presence of different bases in ssDNA, it is necessary to investigate the effect of sequence on the FET-based molecular recognition of DA. SWCNT FETs were decorated with homo- and repeated-base ssDNA sequences, and the electrical response induced by DA in the presence and absence of UA was gauged in terms of the variation in transistor electrical parameters including conductance, transconductance, threshold voltage and hysteresis gap. Our results showed that the response of ssDNA-decorated devices to DA, irrespective of the presence or absence of UA, was DNA sequence dependent and exhibited the trend: G > A > C and GA > GT > AC > CT, for homo- and repeated-base sequences, respectively. The different response of various SWCNT–ssDNA systems to DA underlines the sequence selectivity, whereas the detection of DA in the presence of UA highlights the molecular selectivity of the ssDNA-decorated devices.

Nafion-Radical Hybrid Films on Carbon Nanotube Transistors for Monitoring Antipsychotic Drug Effects on Stimulated Dopamine Release

“…[9][10][11][12][13][14] On the other hand, field-effect transistor (FET) devices have shown distinct advantages, and they have been extensively used to detect dopamine 4 in various environments. [15][16][17][18] However, they were not utilized to evaluate the efficacy of actual antipsychotic drugs as well as real-time cellular measurements. Recently, many reports have shown the successful development of carbon nanotube (CNT)-based FET devices for the detection of biomaterials.…”

Recent advances in nanomaterial-modified electrical platforms for the detection of dopamine in living cells

“…The electrical signals of FETs are generally expressed as the changes in current intensities and are proven to be extremely sensitive to the alterations of the external environment. Even though the fabrication process is quite complex, this extreme sensitivity made the FETs become one of the most popular platforms in the biosensor area [ 87 , 88 ]. Since the amount of DA exocytosed from neuronal cells is extremely low, this advantage of FETs, that is, the high sensitivity, is preferred to construct a DA biosensor.…”