Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai

Abstract

Abstract:We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.

Foodborne Intestinal Flukes in Southeast Asia

“…The sequence comparison of 28S ribosomal DNA and mtCOI of 3 Metagonimus species also supported M. miyatai as a separate species [303]. The length of the rDNA sequence in 28S D1 region of the 3 Metagonimus species was 248 bp and its G+C content was 52% [303].…”

“…The length of the rDNA sequence in 28S D1 region of the 3 Metagonimus species was 248 bp and its G+C content was 52% [303]. The length of the mtCOI sequence averaged 400 bp with a G+C content ranged from 44% in M. miyatai and 46% in M. takahashii to 47% in M. yokogawai [303].…”

“…The length of the rDNA sequence in 28S D1 region of the 3 Metagonimus species was 248 bp and its G+C content was 52% [303]. The length of the mtCOI sequence averaged 400 bp with a G+C content ranged from 44% in M. miyatai and 46% in M. takahashii to 47% in M. yokogawai [303]. Nucleotide sequence differences between species were 23.0% (92/400 bp) between M. miyatai and M. takahashii, 16.2% (65/ 400 bp) between M. miyatai and M. yokogawai, 13.2% (53/400 bp) between M. takahashii and M. yokogawai [303].…”

“…The length of the mtCOI sequence averaged 400 bp with a G+C content ranged from 44% in M. miyatai and 46% in M. takahashii to 47% in M. yokogawai [303]. Nucleotide sequence differences between species were 23.0% (92/400 bp) between M. miyatai and M. takahashii, 16.2% (65/ 400 bp) between M. miyatai and M. yokogawai, 13.2% (53/400 bp) between M. takahashii and M. yokogawai [303]. M. takahashii and M. yokogawai are placed in the same clade supported by the sequence and phylogenetic tree analysis of 28S D1 rDNA and mtCOI gene by neighbor-joining and parsimony method; M. miyatai is placed in a different clade from 2 other Metagonimus species [303].…”

Preliminary study: Genetic population of Calcinus elegans in the South coast of Java Island based on sequence COI Gene

“…This is because the COI gene has sustainable regions and a high variability between species. 13 Genetic diversity of C. elegans can be affected by a discrepancy of spatial factors. 14 The study of some populations of aquatic organisms is determined by spatial factors, including population size, the strength of diffusion, a form of aquatic and behavioral characteristics of larvae, and the model of current movements.…”