Sequence characterization of Dasheen mosaic virus isolates from cocoyam in Nicaragua

Reyes, Guillermo Pedro Morales; Ramsell, J. N. E.; Nyman, M.; Kvarnheden, Anders

doi:10.1007/s00705-008-0257-9

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…The identities between these viruses and the dendrogram clustering of these viruses together (Fig. 3) clearly indicates the close relationship among these viruses, as was also reported earlier (Adams et al, 2005;Reyes et al, 2009;Farreyrol et al, 2006). DsMV isolates from the three edible aroids under study differed from two other DsMV isolates (AY994104, AY994105) and with one VMV (AJ616719) from GenBank, in lacking 36 nucleotides at the 5'-end region of the CP and 54 nucleotides (140-194) compared to 9 DsMV isolates (AM910398, DMU00122, AF169832, EF199550, DQ925464, AM910407, AM910401, AM994104, and AY994105) and one VMV isolate (AJ616719) (data not shown).…”

Section: Resultssupporting

confidence: 63%

“…Partial sequence characterization of DsMV infecting Colocasia (Pappu et al,1994), Xanthosoma (Reyes et al, 2009) and Zantedeschia (Chen et al, 2001) and complete genome sequence from Zantedeschia have been reported (Chen et al, 2001). However no sequence information of DsMV from A. paeoniifolius, C. esculenta, and X. sagittifolium in India has so far been reported.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Babu¹,

Hegde²

2014

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Summary. -Dasheen mosaic virus (DsMV) infecting three major edible aroids namely Amorphophallus paeoniifolius, Colocasia esculenta, and Xanthosoma sagittifolium cultivated in India was characterized. Infected plants showing typical DsMV symptoms were subjected to reverse transcription-polymerase chain reaction, and an amplification of a 963 bp fragment which encoded the coat protein (CP) gene was obtained. BLAST analysis of the cloned DNA amplicon revealed the identity of the virus to be that of DsMV. Sequence identity matrix of the nucleotide sequences among the three isolates showed that the DsMV isolate infecting A. paeoniifolius and C. esculenta shared an identity as high as 93%, while the DsMV isolate from X. sagittifolium shared an identity of only 73% and 76% with the DsMV isolates from A. paeoniifolius and C. esculenta, respectively. Comparative analysis of the coat protein of the three DsMV isolates showed the presence of DVG motif (A. paeoniifolius and C. esculenta) and DTG motif in X. sagittifolium and several varying potential threonine and asparagine rich N-glycosylation motifs. Single amino acid substitution of the several conserved motifs occurs in all the three DsMV isolates. This is the first characterization of DsMV isolates infecting A. paeoniifolius, C. esculenta, and X. sagittifolium plants in India.

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Section: Resultssupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Babu¹,

Hegde²

2014

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“…S3 ). This sequence repeat has previously been reported in DsMV Nicaraguan isolates and DsMV-LA 18 . Most of the CP of DsMV isolates from taro in Ethiopia, A. konjac in Japan, and two isolates from Zantedeschia aethiopica in Zhejiang province of China possess a similar N-terminal and conserved motif rich in asparagine residues (Fig.…”

Section: Resultssupporting

confidence: 76%

Occurrence and characterization of viruses infecting Amorphophallus in Yunnan, China

Dong,

Zhu,

et al. 2024

Sci Rep

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Viral diseases are becoming an important problem in Amorphophallus production due to the propagation of seed corms and their trade across regions. In this study, combined-High-Throughput Sequencing, RT-PCR, electron microscopy, and mechanical inoculation were used to analyze virus-like infected Amorphophallus samples in Yunnan province to investigate the distribution, molecular characterization, and diversity and evolution of Amorphophallus-infecting viruses including three isolates of dasheen mosaic virus and three orthotospoviruses: mulberry vein banding associated virus (MVBaV), tomato zonate spot virus (TZSV) and impatiens necrotic spot virus (INSV). The results showed that DsMV is the dominant virus infecting Amorphophallus, mixed infections with DsMV and MVBaV to Amorphophallus were quite common in Yunnan province, China. This is the first report on infection of Amorphophallus with MVBaV, TZSV, and impatiens necrotic spot virus (INSV) in China. This work will help to develop an effective integrated management strategy to control the spread of Amorphophallus viral diseases.

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“…The RNA of DsMV is polyadenylated (polyA) at 3′ end, the so called poly-A tail similar to cellular mRNA. Partial genome sequences in the 3′ end of DsMV, particularly involving the NIb, CP and 3′ UTR, from Colocasia, Xanthosoma, Zantedeschia and Amorphophallus, have been determined (Pappu et al, 1994;Babu et al, 2010Babu et al, , 2011Reyes et al, 2009;Chen et al, 2001). There is a single report on the determination of complete genome sequence of DsMV infecting Zantedeschia from China (Chen et al, 2001).…”

Section: Introductionmentioning

confidence: 95%

Whole transcriptome sequencing of diseased elephant foot yam reveals complete genome sequence of Dasheen mosaic virus

et al. 2015

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Dasheen mosaic virus (DsMV) the main causative agent of mosaic disease in elephant foot yam (Amorphophallus paeoniifolius) belongs to Potyviridae. The complete genome sequence of DsMV infecting A. paeoniifolius in Kerala state, India was assembled from the whole transcriptome sequencing reads of diseased host samples. The sequence of the virus which showed 83% identity with DsMV infecting Zantedeschia aethiopica (China) was confirmed through amplification and sequencing with primers designed based on the assembled sequence. Unambiguous recombination events mainly confined to the 5′ terminal 4000 nucleotides of the genome with phylogenetically related potyviruses were recognized. The length and genome composition towards the 3′ end comprising NIb, CP and 3′ UTR were not dependent on the host or the source region.

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Sequence characterization of Dasheen mosaic virus isolates from cocoyam in Nicaragua

Cited by 6 publications

References 12 publications

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Occurrence and characterization of viruses infecting Amorphophallus in Yunnan, China

Whole transcriptome sequencing of diseased elephant foot yam reveals complete genome sequence of Dasheen mosaic virus

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