Sequence Characterization in 3′-Flanking Region of Bovine TNF-α: Association with Milk Production Traits and Somatic Cell Score in Holstein Cattle of Iran

Muhaghegh-Dolatabady, M.; Rezaei, Azam Rahimi

doi:10.21859/ijb.1195

Cited by 3 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the 5 flanking region, as in the promoter region, DNA polymorphisms can affect the pace of transcription and thus the formation of protein products. In several cases, polymorphisms in or near the 5 flanking region of genes in farm animals have been found to modify production traits [52]. Depending on the number of repeats, microsatellites located in the 5 flanking region of the genes modified the expression of the mouse GH receptor gene [53], the goat growth hormone gene [54] and the tilapia PRL gene [55].…”

Section: Discussionmentioning

confidence: 99%

The Role of PRLR Gene Polymorphisms in Milk Production in European Wild Rabbit (Oryctolagus cuniculus)

Benedek

Altbäcker

Zsolnai

et al. 2023

Animals

View full text Add to dashboard Cite

One of the problematic points of rabbit breeding is that the nutritional requirements of the kits are not fully satisfied by the does’ milk production from the third week of lactation onwards. The prolactin receptor gene has a significant effect on reproductive processes, and its polymorphisms have been associated with milk production in several species (cattle, goats, sheep, and buffalo). The European wild rabbit (Oryctolagus cuniculus), has a more diverse genetic background compared to domesticated lines. In the course of our study, sequencing of the 1210 bp long segment of the PRLR gene promoter region was accomplished. We detected four point mutations (SNP1-407G > A, SNP2-496G > C, SNP3-926T> and SNP4-973A > C) and one microsatellite at position 574. In our population, the four SNPs were segregated into four genotypes: AACCCCCC, GGGGTTAA, AAGGTTAC, and GGGGTCAC. Our results show that the genotype in the homozygous form is associated with higher milk production (1564.7 ± 444.7 g) compared to the other three genotypes (AACCCCCC 1399.1 ± 326.8 g; GTGACCTT 1403.8 ± 517.1 g; GGGGTCAC 1220.0 ± 666.2 g), and the short microsatellite repeat (167 bp) also coincides with significantly higher milk production (1623.8 ± 525.1 g). These results make the marker-assisted selection possible also for domesticated lines.

show abstract

Section: Discussionmentioning

confidence: 99%

The Role of PRLR Gene Polymorphisms in Milk Production in European Wild Rabbit (Oryctolagus cuniculus)

Benedek

Altbäcker

Zsolnai

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

Genetic variation in transcription factors and photosynthesis light-reaction genes regulates photosynthetic traits

Wang

Xie

et al. 2018

Tree Physiology

View full text Add to dashboard Cite

Transcription factors (TFs) play crucial roles in regulating the production of the components required for photosynthesis; elucidating the mechanisms by which underlying genetic variation in TFs affects complex photosynthesis-related traits may improve our understanding of photosynthesis and identify ways to improve photosynthetic efficiency. Promoter analysis of 96 nuclear-encoded Populus tomentosa Carr. genes within this pathway revealed 47 motifs responsive to light, stress, hormones and organ-specific regulation, as well as 86 TFs that might bind these motifs. Using phenotype-genotype associations, we identified 244 single-nucleotide polymorphisms (SNPs) within 105 genes associated with 12 photosynthesis-related traits. Most (30.33%) of these SNPs were located in intronic regions and these SNPs explained 18.66% of the mean phenotypic variation in the photosynthesis-related traits. Additionally, expression quantitative trait loci (eQTL) mapping identified 216 eQTLs associated with 110 eGenes (genes regulated by eQTLs), explaining 14.12% of the variability of gene expression. The lead SNPs of 12.04% of the eQTLs also contributed to phenotypic variation. Among these, a SNP in zf-Dof 5.6 (G120_9287) affected photosynthesis by modulating the expression of a sub-regulatory network of eight other TFs, which in turn regulate 55 photosynthesis-related genes. Furthermore, epistasis analysis identified a large interacting network representing 732 SNP-SNP pairs, of which 354 were photosynthesis gene-TF pairs, emphasizing the important roles of TFs in affecting photosynthesis-related traits. We combined eQTL and epistasis analysis and found 32 TFs harboring eQTLs being epistatic to their targets (identified by eQTL analysis), of which 15 TFs were also associated with photosynthesis traits. We therefore constructed a schematic model of TFs involved in regulating the photosynthetic light reaction pathway. Taken together, our results provide insight into the genetic regulation of photosynthesis, and may drive progress in the marker-assisted selection of desirable P. tomentosa genotypes with more efficient photosynthesis.

show abstract

Association between Single Nucleotide Ppolymorphism (SNP) in the Intronic Region of (C2239T) B4-Defensin Gene with Milk Production Traits and Somatic Cell Count in Holstein Cattle

dolatabady

rahimirezaei²

2021

rap

View full text Add to dashboard Cite

Sequence Characterization in 3′-Flanking Region of Bovine TNF-α: Association with Milk Production Traits and Somatic Cell Score in Holstein Cattle of Iran

Cited by 3 publications

References 23 publications

The Role of PRLR Gene Polymorphisms in Milk Production in European Wild Rabbit (Oryctolagus cuniculus)

The Role of PRLR Gene Polymorphisms in Milk Production in European Wild Rabbit (Oryctolagus cuniculus)

Genetic variation in transcription factors and photosynthesis light-reaction genes regulates photosynthetic traits

Association between Single Nucleotide Ppolymorphism (SNP) in the Intronic Region of (C2239T) B4-Defensin Gene with Milk Production Traits and Somatic Cell Count in Holstein Cattle

Contact Info

Product

Resources

About