Sequence-based and monoclonal antibody typing of Legionella pneumophila isolated from patients in Portugal during 1987-2008

Chasqueira, Maria Jesus; Rodrigues, Luís; Nascimento, Marta; Marques, Teresa

doi:10.2807/ese.14.28.19271-en

Cited by 20 publications

(13 citation statements)

References 11 publications

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“…As other studies have shown [20,21,29,30,31], the presented confirms that the routine analysis of environmental strains may be an important strategy for preventing outbreaks of legionellosis, in association with clinical surveillance data.…”

Section: Discussionsupporting

confidence: 62%

See 1 more Smart Citation

Genotypic diversity of Legionella pneumophila in environmental and clinical strains assessed by Sequence-Based Typing, in association with retrospective clinical surveillance in Northern Italy

Bianchi

Pregliasco

Consonni

et al. 2016

Ann Agric Environ Med.

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Section: Discussionsupporting

confidence: 62%

“…The results were compared with those in the EWGLI database [25] which collates details of L. pneumophila strains, serogroups, monoclonal antibody subgroups and SBT profiles from all over the world [8,18,19,20,21], and with the authors'previous data [22,23,24].…”

Section: Methodsmentioning

confidence: 99%

Genotypic diversity of Legionella pneumophila in environmental and clinical strains assessed by Sequence-Based Typing, in association with retrospective clinical surveillance in Northern Italy

Bianchi

Pregliasco

Consonni

et al. 2016

Ann Agric Environ Med.

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“…ST1 (1, 4, 3, 1, 1, 1) is distributed broadly throughout the world (3,8,20,44) and was the predominant profile in this study. However, the profile of ST-K1 (7,12,17,3,35,11) was not detected in the HPA/EWGLI SBT database or in any other studies.…”

Section: Discussionmentioning

confidence: 96%

Distribution ofLegionellaSpecies from Environmental Water Sources of Public Facilities and Genetic Diversity ofL. pneumophilaSerogroup 1 in South Korea

Lee

Shim

Kim

et al. 2010

Appl Environ Microbiol

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A total of 560 Legionella species were isolated from environmental water sources from public facilities from June to September 2008 throughout South Korea. The distribution of Legionella isolates was investigated according to geographical region, facility type, and sample type. The genetic diversity of 104 isolates of Legionella pneumophila serogroup 1 (sg 1) was analyzed by sequence-based typing (SBT). L. pneumophila was distributed broadly throughout Korea, accounting for 85.0% of the isolates, and L. pneumophila sg 1 predominated in all of the public facilities except for the springs. Legionella anisa and Legionella bozemanii predominated among non-L. pneumophila species (48.1% and 21.0%, respectively). The second most dominant strain differed depending on the facility type: L. anisa was the second most dominant strain in the buildings (10.8%), L. pneumophila sg 5 in public baths (21.6%), L. pneumophila sg 6 in factories (12.0%), and L. pneumophila sg 7 in hospitals (13.1%). In the SBT analysis, 104 L. pneumophila sg 1 isolates were differentiated into 26 sequence types (STs) and categorized into 3 clonal groups (CGs) and 10 singleton STs via the eBURST V3 program. ST1, a potential founder of major CG1, was commonly distributed (48.1%). The dominant ST in hot water was ST-K1 (7, 12, 17, 3, 35, 11, 11), which was designated in this study (36.1%). The second most dominant strain differed depending on the type of facility from which the samples were obtained. The unique allelic profile of ST-K1, obtained from hot water, was not found in the European Working Group for Legionella Infections (EWGLI) SBT database.

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“…According to the studies of the distribution of Lp1 STs in different regions of the world, it appears that the most prevalent ST among clinical and environmental populations of Lp1 is either ST1 (8,(27)(28)(29)(30)(31)(32) or an ST that is confined to a specific geographic region (33)(34)(35). For example, in Belgium and the United Kingdom, the most frequently detected ST among clinical isolates was ST47 (9,35).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Sequence Types among Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 in the United States from 1982 to 2012

et al. 2014

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cSince the establishment of sequence-based typing as the gold standard for DNA-based typing of Legionella pneumophila, the Legionella laboratory at the Centers for Disease Control and Prevention (CDC) has conducted routine sequence-based typing (SBT) analysis of all incoming L. pneumophila serogroup 1 (Lp1) isolates to identify potential links between cases and to better understand genetic diversity and clonal expansion among L. pneumophila bacteria. Retrospective genotyping of Lp1 isolates from sporadic cases and Legionnaires' disease (LD) outbreaks deposited into the CDC reference collection since 1982 has been completed. For this study, we compared the distribution of sequence types (STs) among Lp1 isolates implicated in 26 outbreaks in the United States, 571 clinical isolates from sporadic cases of LD in the United States, and 149 environmental isolates with no known association with LD. The Lp1 isolates under study had been deposited into our collection between 1982 and 2012. We identified 17 outbreak-associated STs, 153 sporadic STs, and 49 environmental STs. We observed that Lp1 STs from outbreaks and sporadic cases are more similar to each other than either group is to environmental STs. The most frequent ST for both sporadic and environmental isolates was ST1, accounting for 25% and 49% of the total number of isolates, respectively. The STs shared by both outbreak-associated and sporadic Lp1 included ST1, ST35, ST36, ST37, and ST222. The STs most commonly found in sporadic and outbreak-associated Lp1 populations may have an increased ability to cause disease and thus may require special attention when detected.

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Sequence-based and monoclonal antibody typing of Legionella pneumophila isolated from patients in Portugal during 1987-2008

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Cited by 20 publications

References 11 publications

Genotypic diversity of Legionella pneumophila in environmental and clinical strains assessed by Sequence-Based Typing, in association with retrospective clinical surveillance in Northern Italy

Genotypic diversity of Legionella pneumophila in environmental and clinical strains assessed by Sequence-Based Typing, in association with retrospective clinical surveillance in Northern Italy

Distribution ofLegionellaSpecies from Environmental Water Sources of Public Facilities and Genetic Diversity ofL. pneumophilaSerogroup 1 in South Korea

Prevalence of Sequence Types among Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 in the United States from 1982 to 2012

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