Sequence and Transcriptional Analysis of the Ubiquitin Gene Cluster in the Genome of Spodoptera exigua Nucleopolyhedrovirus

Strien, E.A. van; Jansen, Bas; Mans, R. M. W.; Zuidema, D.; Vlak, Just M.

doi:10.1099/0022-1317-77-9-2311

Cited by 16 publications

(11 citation statements)

References 35 publications

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“…1, nt 1368-1389) were observed. Similar repeats were observed in other locations in the SpliMNPV genome, as well as in the SeMNPV genome in the 5h nontranslated region of the p10 gene (Zuidema et al, 1993) and in the intergenic region between the ORF v-ubi and xb187 (van Strien et al, 1996). The function of these repeats, if any, is unknown.…”

Section: The Splimnpv P10 Gene Is Separated From the P74 Gene By A 94supporting

confidence: 48%

“…6, the p26-p10-p74 gene cluster is oriented a such a way that the p74 gene is closer to the polyhedrin gene than the p10 gene, except for SpliMNPV in which the opposite localization occurs. The position of the vubi-xb187 cluster (van Strien et al, 1996) also differs in the SpliMNPV genome relative to its position in other baculoviruses. In SpliMNPV, ORF552, which resembles xb187, is located upstream of the p10 gene and no v-ubi is found, either next to ORF552 or in the 1000 nt upstream sequences.…”

Section: Discussionmentioning

confidence: 99%

“…A unique feature of the presumed p10 protein is its size, 11 130 kDa, resulting from an extended coiled-coil structure with ten heptad repeats. This (Croizier & Croizier, 1994) and CfMNPV (Qiu et al, 1996) and as described previously (van Strien et al, 1996) ; p10 gene (p10) as described in Fig. 4 ; ORF552 and its similar ORFs in SeMNPV (xb187), AcMNPV (ORF34 ; Ayres et al, 1994) and OpMNPV (ORF26) as described previously (Ahrens et al, 1997) ; ubiquitin gene (v-ubi) as described previously (van Strien et al, 1996) ; ecdysteroid UDPglucosyltransferase gene (egt) in SpliMNPV (Faktor et al, 1995), CfMNPV (Barrett et al, 1995) and as descried previously (van Strien et al, 1996) ; protein kinase gene (pk) in SpliMNPV (Faktor et al, 1996), SeMNPV (E. A. van Strien & J. G. M. Heldens, personal communication), AcMNPV (Ayres et al, 1994) and OpMNPV (Ahrens et al, 1997) ; ribonucleotide reductase gene (RR1) in SeMNPV (E. A. van Strien, personal communication) and SpliMNPV (O. Faktor, unpublished results) ; p26 and p74 genes in SeMNPV (Zuidema et al, 1993), AcMNPV (Ayres et al, 1994), OpMNPV (Leisy et al, 1986) and CfMNPV (Wilson et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…A comparison of the ORF552 nucleotide sequence with the GenBank and EMBL databases (Table 1) revealed 58n5% identity to the xb187 gene of SeMNPV (van Strien et al, 1996) and equal or less than 48n5 % identity to similar ORFs from OpMNPV (ORF26 ; Ahrens et al, 1997), BmNPV (ORF2 ; GenBank\EMBL accession no. L33180) and AcMNPV (ORF34 ; Ayres et al, 1993).…”

Section: A 552 Bp Orf Resides Upstream Of the P10 Genementioning

confidence: 99%

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The p10 gene of Spodoptera littoralis nucleopolyhedrovirus: nucleotide sequence, transcriptional analysis and unique gene organization in the p10 locus.

Faktor

Toister-Achituv

Nahum

et al. 1997

Journal of General Virology

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The p10 gene of the Spodoptera littoralis (Spli) multicapsid nucleopolyhedrovirus (MNPV) was identified. With a coding sequence of 315 nucleotides (nt), corresponding to a protein of 104 amino acids, the SpliMNPV p10 gene is the longest p10 gene known. This gene codes for a putative protein with an M r of 11 130 and was found to be most closely related to the Spodoptera exigua (Se) MNPV p10 (49n4 % amino acid identity) and most distant from the Autographa californica (Ac) MNPV p10 (20n0% amino acid identity). Characterization of the protein's secondary structure and a comparison with other p10 protein species suggested that this p10 has an extended alpha-helical domain with high probability of forming a large coiled-coil structure. The p10 mRNA was about 1500 nt long, as de-

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Section: The Splimnpv P10 Gene Is Separated From the P74 Gene By A 94supporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: A 552 Bp Orf Resides Upstream Of the P10 Genementioning

confidence: 99%

See 2 more Smart Citations

The p10 gene of Spodoptera littoralis nucleopolyhedrovirus: nucleotide sequence, transcriptional analysis and unique gene organization in the p10 locus.

Faktor

Toister-Achituv

Nahum

et al. 1997

Journal of General Virology

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“…This percentage of identity is lower than that observed among eukaryotic ubiquitin genes (approximately 96%). Several baculoviruses also encode ubiquitin genes (vubi) which are among the most divergent known (76% amino acid identity to the mammalian ubiquitin consensus) (61,160,194).…”

Section: Continued On Facing Pagementioning

confidence: 99%

The Genome of Melanoplus sanguinipes Entomopoxvirus

Afonso

Tulman

et al. 1999

J Virol

194

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The family Poxviridae contains two subfamilies: theEntomopoxvirinae (poxviruses of insects) and theChordopoxvirinae (poxviruses of vertebrates). Here we present the first characterization of the genome of an entomopoxvirus (EPV) which infects the North American migratory grasshopper Melanoplus sanguinipes and other important orthopteran pests. The 236-kbp M. sanguinipes EPV (MsEPV) genome consists of a central coding region bounded by 7-kbp inverted terminal repeats and contains 267 open reading frames (ORFs), of which 107 exhibit similarity to previously described genes. The presence of genes not previously described in poxviruses, and in some cases in any other known virus, suggests significant viral adaptation to the arthropod host and the external environment. Genes predicting interactions with host cellular mechanisms include homologues of the inhibitor of apoptosis protein, stress response protein phosphatase 2C, extracellular matrixin metalloproteases, ubiquitin, calcium binding EF-hand protein, glycosyltransferase, and a triacylglyceride lipase. MsEPV genes with putative functions in prevention and repair of DNA damage include a complete base excision repair pathway (uracil DNA glycosylase, AP endonuclease, DNA polymerase β, and an NAD+-dependent DNA ligase), a photoreactivation repair pathway (cyclobutane pyrimidine dimer photolyase), a LINE-type reverse transcriptase, and a mutT homologue. The presence of these specific repair pathways may represent viral adaptation for repair of environmentally induced DNA damage. The absence of previously described poxvirus enzymes involved in nucleotide metabolism and the presence of a novel thymidylate synthase homologue suggest that MsEPV is heavily reliant on host cell nucleotide pools and the de novo nucleotide biosynthesis pathway. MsEPV and lepidopteran genus B EPVs lack genome colinearity and exhibit a low level of amino acid identity among homologous genes (20 to 59%), perhaps reflecting a significant evolutionary distance between lepidopteran and orthopteran viruses. Divergence between MsEPV and theChordopoxvirinae is indicated by the presence of only 49 identifiable chordopoxvirus homologues, low-level amino acid identity among these genes (20 to 48%), and the presence in MsEPV of 43 novel ORFs in five gene families. Genes common to both poxvirus subfamilies, which include those encoding enzymes involved in RNA transcription and modification, DNA replication, protein processing, virion assembly, and virion structural proteins, define the genetic core of the Poxviridae.

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Auxiliary Genes of Baculoviruses

O’Reilly

1997

The Baculoviruses

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Sequence and Transcriptional Analysis of the Ubiquitin Gene Cluster in the Genome of Spodoptera exigua Nucleopolyhedrovirus

Cited by 16 publications

References 35 publications

The p10 gene of Spodoptera littoralis nucleopolyhedrovirus: nucleotide sequence, transcriptional analysis and unique gene organization in the p10 locus.

The p10 gene of Spodoptera littoralis nucleopolyhedrovirus: nucleotide sequence, transcriptional analysis and unique gene organization in the p10 locus.

The Genome of Melanoplus sanguinipes Entomopoxvirus

Auxiliary Genes of Baculoviruses

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