Sequence and phylogenetic analysis of the large (L) segment of the Tahyna virus genome

Quinan, Bárbara Resende; Magalhães, Cíntia Lopes de Brito; Novaes, Renata Franco Vianna; Santos, João Rodrigues dos; Kroon, Erna Geessien; Bonjardim, Cláudio Antônio; Ferreira, Paulo César Peregrino

doi:10.1007/s11262-008-0212-6

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…The tree showed that the Tospovirus clade was closer to the Orthobunyavirus genus, followed by the Hantavirus and Nairovirus genera, similar to the findings by Roberts et al [40] when analyzing the La Crosse L segment. The general topology of this tree based on the complete L sequences is also very similar to that obtained when only partial L sequences were used for phylogenetic analysis [41].…”

Section: Discussionsupporting

confidence: 63%

Tensaw virus genome sequence and its relation to other Bunyaviridae

2009

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Tensaw virus (TSV) belongs to the genus Orthobunyavirus within the Bunyaviridae family. Although TSV does not cause hemorrhagic fever as some other members of its family, serological studies have shown that serum from Florida residents react against TSV indicating viral infection in humans. In this study, the three RNA genome segments of a TSV isolated from Anopheles crucians mosquitoes collected in North Central Florida in 2006 and a TSV isolate obtained from the CDC, Fort Collins, were sequenced and compared to other Bunyaviridae. The placement of the TSVs within the Bunyamwera serogroup was confirmed by phylogenetic analysis of the inferred amino acid (aa) sequence of proteins coded by each of the RNA segments separately as well as by the combined tree of the same three inferred proteins. The N terminal glycoprotein (Gn) encoded by the M segment contained the 18 conserved Cysteines present in Bunyamwera and California serogroups, the two glycosylation sites, and residues considered potential proteolytic cleavage sites conserved in other Bunyaviridae. The TSV L protein displayed all the strictly conserved amino acids in the four conserved regions known to be catalytically active for the RNA dependent RNA polymerase transcriptase and replicase activities. The amino acid conservation between the two TSV viral isolates was 100, 99.4, and 99.6% for the S, M, and L segments, respectively.

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Section: Discussionsupporting

confidence: 63%

Tensaw virus genome sequence and its relation to other Bunyaviridae

2009

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“…The BUNV L protein is 259 kDa and contains 2238 aa (Elliott, 1989). Although no extended homology is found with other negative-stranded virus polymerases or even with the polymerases of viruses in other genera in the family Bunyaviridae , the motifs conserved in other RdRps that comprise the ‘polymerase module’ have been identified (Aquino et al , 2003; Elliott, 1989; Gowda et al , 1998; Muller et al , 1994; Poch et al , 1989; Quinan et al , 2008; Roberts et al , 1995; van Poelwijk et al , 1997). Its role as the viral polymerase was confirmed by the ability of the L protein to transcribe authentic BUNV RNP templates (Jin & Elliott, 1991).…”

Section: Introductionmentioning

confidence: 99%

Generation and analysis of recombinant Bunyamwera orthobunyaviruses expressing V5 epitope-tagged L proteins

Shi

Elliott

2009

Journal of General Virology

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The L protein of Bunyamwera virus (BUNV; family Bunyaviridae) is an RNA-dependent RNA polymerase, 2238 aa in length, that catalyses transcription and replication of the negative-sense, tripartite RNA genome. To learn more about the molecular interactions of the L protein and to monitor its intracellular distribution we inserted a 14 aa V5 epitope derived from parainfluenza virus type 5, against which high-affinity antibodies are available, into different regions of the protein. Insertion of the epitope at positions 1935 or 2046 resulted in recombinant L proteins that retained functionality in a minireplicon assay. Two viable recombinant viruses, rBUNL4V5 and rBUNL5V5, expressing the tagged L protein were rescued by reverse genetics, and characterized with respect to their plaque size, growth kinetics and protein synthesis profile. The recombinant viruses behaved similarly to wild-type (wt) BUNV in BHK-21 cells, but formed smaller plaques and grew to lower titres in Vero E6 cells compared with wt BUNV. Immunofluorescent staining of infected cells showed the L protein to have a punctate to reticular distribution in the cytoplasm, and cell fractionation studies indicated that the L protein was present in both soluble and microsomal fractions. Co-immunoprecipitation and confocal microscopic assays confirmed an interaction between BUNV L and N proteins. The recombinant viruses expressing tagged L protein will be highly valuable reagents for the detailed dissection of the role of the BUNV L protein in virus replication.

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“…4). Despite this difference in length and sequence, nairovirus L proteins still show the four conserved functional regions previously described for other bunyavirus L proteins [94,108,131,139,177]. The bunyavirus L proteins contain the RNA-dependent RNA polymerase (RdRp), and the most conserved region of the L segment among nairoviruses is the region corresponding to the coding sequence for the core catalytic domains of the RdRp [8,85,108].…”

Section: The L Proteinmentioning

confidence: 97%

“…Nairoviruses share many of their features with other bunyaviruses, e.g., replication in the cytoplasm, budding in the Golgi, and their coding and RNA replication strategy. From phylogenetic studies, the members of the genus Nairovirus appear to be most closely related to those of the genus Phlebovirus of all bunyaviruses [108,131,139,177]. However, nairoviruses possess many features not found in other bunyaviruses.…”

Section: Final Remarksmentioning

confidence: 99%

The molecular biology of nairoviruses, an emerging group of tick-borne arboviruses

Lasecka

Baron

2013

Arch Virol

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The nairoviruses are a rapidly emerging group of tick-borne bunyaviruses that includes pathogens of humans (Crimean-Congo hemorrhagic fever virus [CCHFV]) and livestock (Nairobi sheep disease virus [NSDV], also known as Ganjam virus), as well as a large number of viruses for which the normal vertebrate host has not been established. Studies on this group of viruses have been fairly limited, not least because CCHFV is a BSL4 human pathogen, restricting the number of labs able to study the live virus, while NSDV, although highly pathogenic in naive animals, is not seen as a threat in developed countries, making it a low priority. Nevertheless, recent years have seen significant progress in our understanding of the biology of these viruses, particularly that of CCHFV, and this article seeks to draw together our existing knowledge to generate an overall picture of their molecular biology, underlining areas of particular ignorance for future studies.

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Sequence and phylogenetic analysis of the large (L) segment of the Tahyna virus genome

Cited by 5 publications

References 9 publications

Tensaw virus genome sequence and its relation to other Bunyaviridae

Tensaw virus genome sequence and its relation to other Bunyaviridae

Generation and analysis of recombinant Bunyamwera orthobunyaviruses expressing V5 epitope-tagged L proteins

The molecular biology of nairoviruses, an emerging group of tick-borne arboviruses

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