Sequence and phylogenetic analyses of human rotavirus strains: Comparison of VP7 and VP8∗ antigenic epitopes between Tunisian and vaccine strains before national rotavirus vaccine introduction

Fredj, M. Ben; BenHamida-Rebaï, Meriam; Heylen, Elisabeth; Zeller, Mark; Moussa, Amal; Kacem, Saoussen Bel Hadj; Ranst, Marc Van; Matthijnssens, Jelle; Trabelsi, Abdelhalim

doi:10.1016/j.meegid.2013.05.008

Cited by 30 publications

(8 citation statements)

References 76 publications

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“…Samples that remained untypeable after this first VP7-typing assay were further tested with a second PCR assay aiming to detect G12 VP7 specificity. Indeed, to detect the G12 genotype, we optimized a second semi-nested reaction using a G12 specific primer (Samajdar et al, 2006) and, as a positive control, a Tunisian G12 RVA strain which was previously confirmed by sequencing (Ben Hadj Fredj et al, 2013a). The size of the resultant amplicon allows determination of the VP7 genotype.…”

Section: Methodsmentioning

confidence: 99%

“…In fact, G12 RVA strains may have been circulating in Tunisia prior to this study but their detection was not achieved by previously used RT-PCR genotyping, as the G12 -specific primer was included in the PCR mixture. The first detection of a G12 genotype was performed by sequencing of the ninth gene segment of the VP7 untypeable strain (Ben Hadj Fredj et al, 2013a). Such a finding allowed us to further optimize an RT-PCR genotyping protocol that is able to detect the G12 strains.…”

Section: G/p-typingmentioning

confidence: 99%

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Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12

Moussa

Fredj

Fodha

et al. 2016

Journal of Medical Microbiology

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Group A rotavirus (RVA) represents the most important aetiological agent of diarrhoea in children worldwide. From January 2009 to December 2014, a multi-centre study realized through 11 Tunisian cities was undertaken among children aged <5 years consulting or hospitalized for acute gastroenteritis. A total of 1127 faecal samples were collected. All samples were screened by ELISA for the presence of RVA antigen. RVA-positive samples were further analyzed by PAGE and used for G/P-genotyping by semi-nested multiplex RT-PCR. Globally, 270 specimens (24 %) were RVA-positive, with peaks observed annually between November and March. Nine different electropherotypes could be visualized by PAGE, six with a long profile (173 cases) and two with a short one (seven cases). Mixed profiles were detected in two cases. Among the 267 VP7 genotyped strains, the predominant G-genotype was G1 (39.6 %) followed by G3 (22.2 %), G4 (13 %), G9 (11.5 %), G2 (5.2 %) and G12 (5.2 %). Among the 260 VP4 genotyped strains, P[8] genotype was the predominant (74. 9 %). Uncommon G/Pgenotype combinations, mixed infections and untypeable strains were also detected. This is the first report, in Tunisia, of multiple detection of an emerging human RVA strain, G12 genotype. This study highlighted the need for maintaining active surveillance of emerging strains in Northern Africa.

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Section: Methodsmentioning

confidence: 99%

Section: G/p-typingmentioning

confidence: 99%

Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12

Moussa

Fredj

Fodha

et al. 2016

Journal of Medical Microbiology

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“…21,22 Three of these 5 studies investigated the rotavirus strains as pre-and post-vaccination, 2 in Yemen 19,20 and one in Morocco. 21 The studies that investigated the pre-vaccination rotavirus strain prevalence in other EMR countries are from Bahrain, 8 Egypt, 23 Kuwait, 10 Iran, 7,[24][25][26][27] Iraq, 28,29 Jordan, 30 Saudi Arabia, 31,32 Libya, 33 Morocco, 21,34,35 Oman, 36,37 Yemen, 19,20,38,39 Tunisia, [40][41][42][43][44] and Pakistan. [45][46][47][48][49] We excluded other 8 countries of the EMR from this review )Sudan, Somalia, Afghanistan, Qatar, United Arab Emirates, Djibouti, Syria, and Lebanon( because there were no reports on circulating rotavirus strains among children under 5 years of age.…”

Section: The Outcome Measures 1( Primary Outcome)s(mentioning

confidence: 99%

Circulating rotavirus G and P strains post rotavirus vaccination in Eastern Mediterranean Region

Almalki

2018

SMJ

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“…Therefore, it may not be responsible for antigenic alteration in the mutated strain. Of note, several studies on amino acid differences in VP7 and VP4 antigenic sites between wild-type rotaviruses and vaccine strains reported that most of the circulating wild-type strains possessed N instead of S at aa 123 [17, 18]. Therefore, we inferred that the amino acid at aa 123 was likely to have changed the strain closer to that of wild-type strains through multiple replications in intestine during the shedding periods.…”

Section: Resultsmentioning

confidence: 99%

Detection of mutations in the VP7 gene of vaccine-derived strains shed by monovalent rotavirus vaccine recipients

Kaneko

Takanashi

Inoue

et al. 2019

Access Microbiology

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Strains of Rotarix, a live attenuated monovalent oral rotavirus vaccine, replicate in the intestine and are shed for about one month in immunocompetent recipients. The current study aimed to identify genetic changes of shed strains to reveal any significant mutations and their clinical impact on recipients. Stool samples of recipients of the first dose of Rotarix were sequentially collected for one month from the day of administration. Sequence analyses of the VP7 gene in eight recipients revealed five amino acid substitutions. Among them, two were observed in aa123, which is located in antigenic region 7-1a. Since there were no associated clinical symptoms, the genetic changes were unlikely to have caused reversion of pathogenicity of vaccine strain. Of interest, the virus in one case became closer to wild-type rotavirus via an amino acid change at aa123 occurring 14 days after administration, which might have resulted from multiple replications and long-term shedding of the vaccine strain.

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Sequence and phylogenetic analyses of human rotavirus strains: Comparison of VP7 and VP8∗ antigenic epitopes between Tunisian and vaccine strains before national rotavirus vaccine introduction

Cited by 30 publications

References 76 publications

Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12

Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12

Circulating rotavirus G and P strains post rotavirus vaccination in Eastern Mediterranean Region

Detection of mutations in the VP7 gene of vaccine-derived strains shed by monovalent rotavirus vaccine recipients

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