Sequence and phylogenetic analyses of nonstructural protein 2 genes of species B porcine rotaviruses detected in Japan during 2001–2009

Phylogenetic analysis of nonstructural protein 5 (NSP5) gene sequences in porcine rotavirus B strains

“…However, several bovine and human RVB strains that have sequence data available for most of their genome, in addition to a few human RVB and rat RVB (IDIR) strains already published, have been analyzed recently (Eiden et al, 1992;Sen et al, 2001;Ahmed et al, 2004;Yang et al, 2004;Yamamoto et al, 2010;Ghosh et al, 2010). Furthermore, genetic divergence and the classification of NSP1 and NSP2 genes from several porcine RVBs has been elucidated subsequent to the classification of RVB VP7 including porcine RVBs in human, rat, bovine and partial ovine RVBs (Kuga et al, 2009;Suzuki et al, 2011Suzuki et al, , 2012. Further genetic information on the remaining segments of porcine RVBs is required to uncover accurately the status of the molecular evolution of RVBs, since fullgenome analysis of RVBs is important for understanding of not only ecology and evolution of the pathogen, but also the mechanisms involved in genetic diversity such as gene reassortment and/or crossing of the host-species barrier.…”

Molecular Characterization of a New G (VP7) Genotype in Group B Porcine Rotavirus

“…Using an nt cutoff value of 80%, they identified 20 RVB G genotypes. Additionally, using this nt cutoff value proposed by Marthaler et al [12], Suzuki et al [14] described a new RVB G genotype (G21) isolated from pigs in India.…”

“…The relatively few worldwide reports of RVB infection in animals may be attributed to the fact that small quantities of this virus are excreted in the feces of infected animals, which affects the diagnosis of RVB infections [9,12,14].…”

“…Knowledge regarding the genome of porcine RVB remains restricted to information obtained via analyses of the VP6, VP7, NSP1, NSP2, and NSP5 proteins [3,9,11,12,[14][15][16]. Similar to RVA strains, RVB strains are categorized using a sequence-based classification system [12,16,17].…”

“…However, small quantities of this virus are excreted in the feces of infected animals, and it is difficult to adapt RVB strains to cell culture [9,12,14,23]; as a result, the serological and molecular characterization of RVB strains remains limited.…”