Sequence and Mapping of Galectin-5, a β-Galactoside-binding Lectin, Found in Rat Erythrocytes

Abstract

A monomeric rat beta-galactoside-binding lectin previously purified from extracts of rat lung has been localized to erythrocytes, and the cDNA encoding it has been isolated from a rat reticulocyte cDNA library. The deduced amino acid sequence of the cDNA predicts a protein with a M(r) of 16,199, with no evidence of a signal peptide. The deduced sequence is identical to the sequences of seven proteolytic peptides derived from the purified lectin. Peptide analysis by mass spectrometry indicates that the N-terminal methionine is cleaved and that serine 2 is acetylated. The lectin shares all the strictly conserved amino acid residues of other members of the mammalian galectin family and is designated galectin-5 (GenBank accession number L36862). Galectin-5 is a weak agglutinin of rat erythrocytes, despite its monomeric structure. The gene encoding galectin-5 (LGALS5) has been mapped in mouse to chromosome 11, approximately 50 centimorgans from the centromere and 1.8 +/- 1.8 centimorgans from the polymorphic marker D11Mit34n, a region syntenic with human chromosome 17q11.

Stimulation of Proliferation of Rat Hepatic Stellate Cells by Galectin-1 and Galectin-3 through Different Intracellular Signaling Pathways

“…Galectin-1 forms a homodimer of 14-kDa subunits, and each subunit has a single carbohydrate-binding site (51). On the other hand, galectin-3 is a monomer of 32-kDa subunits with a single carbohydratebinding site (52). Galectin-1 has an extensive affinity for complex-type N-glycans.…”

Molecular characterization of prostate carcinoma tumor antigen-1, PCTA-1, a human Galectin-8 related gene

“…Others have been isolated in screens designed to identify molecules associated with speci®c biological phenomena such as carcinogenesis or dierentiation, employing screens using expression cloning approaches e.g. Galectin-5, rat Galectin-8 and PCTA-1 (Gitt et al, 1998a(Gitt et al, , 1995Hadari et al, 1995;Su et al, 1996). PCTA-1 was identi®ed in two independent carcinoma related screens involving prostate cancer surface marker identi®cation and in a lung cancer cDNA library expression screen (Bassen et al, 1999), respectively.…”

Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin.

“…Abbreviations used in this paper: NCM, nitrocellulose membrane; TUNEL, terminal deoxytransferase-mediated dUTP nick end labeling. thiogalactopyranoside), and further cultured for 5 h. To isolate recombinant proteins, bacterial pellets were prepared and the cells were suspended in 100 ml of either MEPBS buffer (PBS with 4 mM ␤ -mercaptoethanol, 2 mM EDTA) (10,13) or Tris-DTT buffer (20 mM Tris, pH 7.4, 5 mM EDTA, 150 mM sodium chloride, 1 mM DTT) (28) containing 1.25% Triton X-100, 10 mM benzamidine, 10 mM ⑀ -amino-n -caproic acid, and 2 mM phenylmethanesulfonyl fluoride. The cells were lysed by sonication and the lysate was centrifuged at 20,000 g at 4 Њ C for 30 min.…”