Sequence and Functional Characterization of Feline Interleukin 2

Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)

“…However, since these latter cytokines are synthesised by monocytes and macrophages this low level expression may result from cell activation during processing (Rottman et al, 1995). After stimulation with Con A, feline PBMCs readily produce mRNA for IL-2, IL-4, IL-6, IL-10, IL-12, IFNg and TNF (Cozzi et al, 1993;Rottman et al, 1996). Our study confirmed this finding for each of these cytokines, except TNF, which was not assessed.…”

“…The optimal dose of Con A has been determined to be $10 mg/ml (Goitsuka et al, 1986;Rottman et al, 1995). Prior to stimulation, feline IL-2 mRNA is not readily detected (Cozzi et al, 1993), while IL-6, IL-10, IL-12 and TNF appear to be expressed constitutively at low levels (Rottman et al, 1995). However, since these latter cytokines are synthesised by monocytes and macrophages this low level expression may result from cell activation during processing (Rottman et al, 1995).…”

“…Primers were designed to the feline housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (G3PDH) after sequencing it. The cytokine primer sequences used initially were those designed by Rottman et al (1995), based on published sequences (IL-2 ± Cozzi et al, 1993;IL-6 ± Bradley et al, 1993;Ohashi et al, 1993). A number of primers were redesigned based on new published data (IL-4 forward (F) and reverse (R); IL-10 (R); IFNg (R); shown on Table 1 (IL-4 ± Schijns et al, 1995b;IFNg ± Argyle et al, 1995;Schijns et al, 1995a).…”

Molecular Cloning, Characterization, Polymorphism, and Association Study of the Interleukin-2 Gene in Indian Crossbred Cattle

“…The cDNA length of the Bos taurus, bubaline, ovine, and caprine IL-2 was observed to be 468 bp, whereas in pigs the size was 465 bp. The primary structure of IL-2 has been deduced in several animal species, including cattle (Cerretti et al 1986), sheep (Goodall et al 1990), pigs (Goodall et al 1991), and cats (Cozzi et al 1993). Comparison of the nucleotide sequence with that of Bos taurus revealed one nucleotide change at position 8, from A in Bos taurus to G in the crossbred cattle.…”

ISOLATION AND CHARACTERIZATION OF A cDNA ENCODING INTERLEUKIN 2 FROM THE FLORIDA MANATEE,TRICHECHUS MANATUS LATIROSTRIS

“…While IL-2 is produced by, and acts upon, T-lymphocytes, it also directly affects growth and differentiation of B-lymphocytes, natural killer cells, lymphokine-activated killer cells, monocytes, macrophages, and oligodendrocytes (Kuziel and Greene 1991). Interleukin-2 cDNA has been cloned and characterized for several species, including human (Taniguchi et al 1983), white-handed gibbon (Chen et al 1985), pig (Goodall et al 1991), sheep (Goodall et al 1990>, horse (Vandergrifft and Horohov 1993), cow (Cerretti et al 1986), rat (McKnight: et al 1989, cat (Cozzi et al 1993), and mouse (Yokota et al 1985). Due to the importance of IL-2 in orchestrating the mammalian immune response, and because very little is known about the genetics and immunology of the endangered manatee, we sought to isolate and characterize manatee IL-2.…”