Sequence and expression of the chicken calcitonin gene

Minvielle, Stéphane; Cressent, M.; Delehaye, M.C.; Segond, N.; Milhaud, G; Jullienne, A.; Moukhtar, M.S.; Lasmoles, F.

doi:10.1016/0014-5793(87)80510-0

Cited by 34 publications

(12 citation statements)

References 24 publications

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“…It also plays important roles in various brain functions in the motor, sensory and integrative systems [7]. In non-mammalian vertebrates, alternative splicing was also demonstrated generating two distinct transcripts for CT and CGRP, such as in the chicken, salmon and fugu [8,9,10]. In these groups, CT is synthesized in one organ, the ultimobranchial body [11], whereas CGRP is present in many tissues [3,12,13,14].…”

Section: Introductionmentioning

confidence: 99%

Evidence for the Presence of Molecules Related to the Neuropeptide CGRP in Two Cephalopods, Sepia officinalis and Nautilus macromphalus: Comparison with Its Target Organ Distribution

Lafont¹,

Dufour²,

Fouchereau-Péron³

2006

Neuroendocrinology

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Calcitonin gene-related peptide (CGRP) is a neuropeptide mainly involved in brain and cardiovascular functions in mammals. We investigated its presence and potential roles in two cephalopods, Sepia officinalis and Nautilus macromphalus. CGRP-like, but not calcitonin (CT)-like, molecules were detected by specific radioimmuno- and radioreceptor assays in the brain, optic lobes, branchial heart or afferent branchial vein and kidney. Gel exclusion chromatography of cephalopod brain extracts, followed by SDS-PAGE, indicated that CGRP-like molecules had a molecular weight of around 3 kDa, close to that of human CGRP. The distribution of CGRP target organs was characterized by binding studies in cuttlefish. Specific CGRP binding sites were detected in the brain, optic lobes, and kidney, indicating potential autocrine/paracrine roles of CGRP. Specific CGRP binding sites were also detected in the gills and shell sac that do not contain the peptide itself, indicating potential endocrine roles of CGRP. Accordingly, high circulating levels of CGRP-like molecules were detected in hemolymph of both cuttlefish and nautilus, unlike the situation in mammals. CGRP binding sites were further characterized in the cuttlefish gills by the Scatchard method. Our study indicates that the brain neurotransmitter role of CGRP could represent an ancient role in metazoa, already present in cephalopods and conserved among vertebrates. In contrast, the endocrine role of CGRP, which was suggested in cephalopods and also present in teleosts, may have been lost during the evolution of the tetrapod lineage. Our data support the hypothesis that CGRP represents the ancestral molecule of the CT/CGRP family appeared in metazoa before the vertebrate emergence.

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Section: Introductionmentioning

confidence: 99%

Evidence for the Presence of Molecules Related to the Neuropeptide CGRP in Two Cephalopods, Sepia officinalis and Nautilus macromphalus: Comparison with Its Target Organ Distribution

Lafont¹,

Dufour²,

Fouchereau-Péron³

2006

Neuroendocrinology

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“…Calcitonin is a 32-amino-acid peptide hor mone that is synthesized by the parafollicular (C cells) of the thyroid gland and the ultimobranchial gland in mammals and nonmam malian vertebrates, respectively [Copp et al, 1962;Minvielle et al. 1987], The primary activity of calcitonin is to decrease circulating levels of calcium and phosphate by inhibiting bone resorption [Lenz et al.…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Alternative RNA Processing in Calcitonin/ Calcitonin Gene-Related Peptide Gene Expression

Emeson

Yeakley

1993

Cell Physiol Biochem

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The alternative splicing of messenger RNA (mRNA) precursors represents a common mechanism for increasing the flexibility of eukaryotic gene expression by the generation of structurally distinct, developmentally regulated and tissue-specific protein isoforms. The calcitonin/calcitonin-gene-related peptide (CGRP) primary RNA transcript undergoes tissue-specific alternative processing, resulting in the differential production of calcitonin mRNA in thyroid C cells and CGRP mRNA in neurons of the central and peripheral nervous systems. The calcitonin/CGRP gene provides an excellent model for examining the regulation of cell-specific alternative RNA processing events in complex transcription units expressed within the neuroendocrine system. Initial studies attempting to delineate the biochemical events involved in the regulation of these cellular processes have taken advantage of a number of molecular biological techniques including expression of the calcitonin/CGRP transcription unit in transgenic mice, tissue culture model systems that mimic the alternative RNA processing choices made by tissues in vivo and the development of in vitro splicing systems. It is anticipated that combinations of these experimental approaches will allow the definition of biochemical processes and the isolation of the cellular machinery involved in the regulation of cell-specific RNA processing events.

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“…This had been initially established in mammals (4)(5)(6) and chicken (7). More recently function of this complex transcription unit has also been demonstrated in salmon (8). We have observed mRNA coding for the two peptides, CT and CT gene-related peptide, in this species (9,10).…”

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confidence: 71%