Sequence and expression of theCAPA/CAP2bgene in the tobacco hawkmoth,Manduca sexta

Loi, Poh Kheng; Tublitz, Nathan J.

doi:10.1242/jeb.01186

Cited by 35 publications

(37 citation statements)

References 32 publications

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“…The abdominal ganglia are most likely expressing the capa gene in addition to some neurons in the SEG. This is a similar pattern that has been described for D. melanogaster (Kean et al 2002) and in the moth M. sexta (Loi and Tublitz 2004). In mosquitoes the PVKs are involved in regulating water balance as they are in D. melanogaster (Pollock et al 2004;Ionescu and Donini 2012).…”

Section: Discussionsupporting

confidence: 81%

“…A similar two gene expression pattern for PK/PBAN-like peptides has been proposed for Solenopsis sp. (Choi et al 2009), Manduca sexta (Loi and Tublitz 2004), Bombyx mori (Roller et al 2008), and Periplaneta americana (Predel and Eckert 2000;Schoofs et al 1992). A search of the Anopholes gambiae genome indicates that these same two genes putatively code for peptides in the PK/PBAN family (Riehle et al 2002) The PKs produced by capa and hugin have been confirmed by mass spectroscopy in the mosquito A. aegypti (Predel et al 2010).…”

Section: Introductionmentioning

confidence: 94%

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Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

2014

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The pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides is characterized by a common C-terminal pentapeptide, FXPRLamide, which is required for diverse physiological functions in various insects. Polyclonal antisera against the C-terminus was utilized to determine the location of cell bodies and axons in the central nervous systems of larval and adult mosquitoes. Immunoreactive material was detected in three groups of neurons in the subesophageal ganglion of larvae and adults. The corpora cardiaca of both larvae and adults contained immunoreactivity indicating potential release into circulation. The adult and larval brains had at least one pair of immunoreactive neurons in the protocerebrum with the adult brain having additional immunoreactive neurons in the dorsal medial part of the protocerebrum. The ventral ganglia of both larvae and adults each contained one pair of neurons that sent their axons to a perisympathetic organ associated with each abdominal ganglion. These results indicate that the mosquito nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph. The peptides in insects and mosquitoes are produced by two genes, capa and pk/pban. Utilizing PCR protocols, we demonstrate that products of the capa gene could be produced in the abdominal ventral ganglia and the products of the pk/pban gene could be produced in the subesophageal ganglion. Two receptors for pyrokinin peptides were differentially localized to various tissues. Abstract Pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides is characterized by a common C-terminal pentapeptide, FXPRLamide, that is required for diverse physiological functions in various insects. Polyclonal antisera against the C-terminus was utilized to determine the location of cell bodies and axons in the central nervous systems of larval and adult mosquitoes. Immunoreactive material was detected in three groups of neurons in the subesophageal ganglion of larvae and adults. The corpora cardiaca of both larvae and adults contained immunoreactivity indicating potential release into circulation. The adult and larval brains had at least one pair of immunoreactive neurons in the protocerebrum with the adult brain having additional immunoreactive neurons in the dorsal medial part of the protocerebrum. The ventral ganglia of both larvae and adults each contained one pair of neurons that sent their axons to a perisympathetic organ associated with each abdominal ganglion. These results indicate that the mosquito nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph. The peptides in insects and mosquitoes are produced by two genes, capa and pk/pban. Utilizing PCR protocols we demonstrate that products of the capa gene could be produced in the abdominal ventral ganglia and the products of the pk/pban gene could be produced in the subesophageal ganglion. Two receptors for pyrokinin peptides were differ...

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 94%

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

2014

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“…This work demonstrated that the Manduca sexta neuropeptide CAP2b, a member of the capa peptide family Loi and Tublitz, 2004) ] i rise was shown to occur via phospholipase Cβ and inositol 1,4,5-trisphosphate receptor (Pollock et al, 2003), and also involves Ca 2+ release via the Golgi sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA) channel . CAP2b was also shown to stimulate Ca 2+ influx through principal cell plasma membrane Ca 2+ channels -transient receptor-like (TRPL), L-type and cyclic nucleotide-gated (CNG) channels (Fig.…”

Section: Calcium Signalling In Tubule Principal Cellsmentioning

confidence: 96%

Cell signalling mechanisms for insect stress tolerance

Davies

Cabrero

Overend

et al. 2014

Journal of Experimental Biology

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Insects successfully occupy most environmental niches and this success depends on surviving a broad range of environmental stressors including temperature, desiccation, xenobiotic, osmotic and infection stress. Epithelial tissues play key roles as barriers between the external and internal environments and therefore maintain homeostasis and organismal tolerance to multiple stressors. As such, the crucial role of epithelia in organismal stress tolerance cannot be underestimated. At a molecular level, multiple cell-specific signalling pathways including cyclic cAMP, cyclic cGMP and calcium modulate tissue, and hence, organismal responses to stress. Thus, epithelial cell-specific signal transduction can be usefully studied to determine the molecular mechanisms of organismal stress tolerance in vivo. This review will explore cell signalling modulation of stress tolerance in insects by focusing on cell signalling in a fluid transporting epithelium -the Malpighian tubule. Manipulation of specific genes and signalling pathways in only defined tubule cell types can influence the survival outcome in response to multiple environmental stressors including desiccation, immune, salt (ionic) and oxidative stress, suggesting that studies in the genetic model Drosophila melanogaster may reveal novel pathways required for stress tolerance.

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“…6a). Mass spectra from these neurons, however, contained additional prominent peptide signals that could be assigned to the products of the capa-gene (Loi and Tublitz 2004). CAPA-peptides (periviscerokinins/CAP 2bs and a single FXPRLamide) are typical of the median neurosecretory cells in the abdominal ganglia of all insects studied so , and are usually stored in abdominal perisympathetic organs.…”

Section: Resultsmentioning

confidence: 99%

“…7 Overview of the Manduca capa prohormone processing as indicated by mass spectrometric analysis of M Lb -neurons and abdominal PSOs. Cleavage sites are represented by black or hatched bars and previously predicted products (Loi and Tublitz 2004) are represented by blue bars. All products of the capa-gene, which were identified by mass spectrometry, are labelled in green.…”

Section: Discussionmentioning

confidence: 99%

Conservation of the function counts: homologous neurons express sequence‐related neuropeptides that originate from different genes

Neupert

Huetteroth

Schachtner

et al. 2009

Journal of Neurochemistry

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By means of single‐cell matrix assisted laser desorption/ionization time‐of‐flight mass spectrometry, we analysed neuropeptide expression in all FXPRLamide/pheromone biosynthesis activating neuropeptide synthesizing neurons of the adult tobacco hawk moth, Manduca sexta. Mass spectra clearly suggest a completely identical processing of the pheromone biosynthesis activating neuropeptide‐precursor in the mandibular, maxillary and labial neuromeres of the subesophageal ganglion. Only in the pban‐neurons of the labial neuromere, products of two neuropeptide genes, namely the pban‐gene and the capa‐gene, were detected. Both of these genes expressed, amongst others, sequence‐related neuropeptides (extended WFGPRLamides). We speculate that the expression of the two neuropeptide genes is a plesiomorph character typical of moths. A detailed examination of the neuroanatomy and the peptidome of the (two) pban‐neurons in the labial neuromere of moths with homologous neurons of different insects indicates a strong conservation of the function of this neuroendocrine system. In other insects, however, the labial neurons either express products of the fxprl‐gene or products of the capa‐gene. The processing of the respective genes is reduced to extended WFGPRLamides in each case and yields a unique peptidome in the labial cells. Thus, sequence‐related messenger molecules are always produced in these cells and it seems that the respective neurons recruited different neuropeptide genes for this motif.

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Sequence and expression of theCAPA/CAP2bgene in the tobacco hawkmoth,Manduca sexta

Cited by 35 publications

References 32 publications

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

Pyrokinin/PBAN-like peptides in the central nervous system of mosquitoes

Cell signalling mechanisms for insect stress tolerance

Conservation of the function counts: homologous neurons express sequence‐related neuropeptides that originate from different genes

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