Sequence and electron paramagnetic resonance analyses of nitrate reductase NarGH from a denitrifying halophilic euryarchaeote <i>Haloarcula marismortui</i>

Yoshimatsu, Katsuhiko; Iwasaki, Toshio; Fujiwara, Taketomo

doi:10.1016/s0014-5793(02)02524-3

Cited by 49 publications

(51 citation statements)

References 37 publications

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“…In general, the characteristics of these enzymes showed marked resemblance with the bacterial NarGH complex, underscoring the fact that there was a relevant difference related to the subcellular localization between the halophilic and bacterial enzymes (Yoshimatsu, Iwasaki, & Fujiwara 2002;Martinez-Espinosa et al, 2007).…”

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 91%

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Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Microbial Physiology

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A number of species of Haloferax genus (halophilic Archaea) are able to grow micro aerobically or even anaerobically using different alternative electron acceptors such as fumarate, nitrate, chlorate, dimethyl sulphoxide, sulphide and/or trimethylamine. This metabolic capability is also shown by other species of the Halobacteriaceae and Haloferacaceae families (Archaea domain) and it has been mainly tested by physiological studies where cells growth is observed under anaerobic conditions in the presence of the mentioned compounds. This work summarises the main reported features on anaerobic metabolism in the Haloferax, one of the better described haloarchaeal genus with significant potential uses in Biotechnology and Bioremediation. Special attention has been paid to denitrification, also called nitrate respiration. This pathway has been studied so far from KEY WORDSHaloarchaea, denitrification, anaerobic metabolism, Nox emissions, bioremediation.

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Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 91%

“…Haloarcula marismortui (Yoshimatsu et al, 2002), however, the its regulation has not been explored yet.…”

Section: Genes Coding For the Enzymes Sustaining Denitrificationmentioning

confidence: 99%

Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Microbial Physiology

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“…Recently, the similarity of Nar to selenate reductases has also been indicated on the basis that both enzymes have Asp ligands to the Mo atom (Jormakka et al, 2004). In addition, the N-terminal region of NarG includes a typical twin-arginine signal peptide for protein translocation across the membrane by the Tat export pathway and the enzyme has activity in situ with both membranepermeable benzyl viologen and membrane-impermeable methyl viologen, suggesting that the catalytic site is located in the outside of the membrane, as described for the T. selenatis selenate reductase (Yoshimatsu et al, 2002). In contrast, bacterial NarG faces the cytoplasmic side of the membrane and nitrate reduction takes places in the cytoplasm (Berks et al, 1995;Moreno-Vivián et al, 1999).…”

Section: Respiratory Nitrate Reduction In Archaeamentioning

confidence: 97%

“…The enzyme was first described as a homotetramer of a 63 kDa polypeptide (Yoshimatsu et al, 2000), but further characterization revealed that it is a heterodimer with 117 and 47 kDa subunits, the 63 kDa band being an incomplete denaturation state of the enzyme (Yoshimatsu et al, 2002). It has been proposed that the H. marismortui Nar is a new archaeal type of membranebound nitrate reductase (Yoshimatsu et al, 2002). First, the enzyme is composed of two subunits, which are homologous to the NarGH bacterial subunits, but lacks the NarI membrane-associated protein.…”

Section: Respiratory Nitrate Reduction In Archaeamentioning

confidence: 99%

Nitrate reduction and the nitrogen cycle in archaea

2004

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The nitrogen cycle (N-cycle) in the biosphere, mainly driven by prokaryotes, involves different reductive or oxidative reactions used either for assimilatory purposes or in respiratory processes for energy conservation. As the N-cycle has important agricultural and environmental implications, bacterial nitrogen metabolism has become a major research topic in recent years. Archaea are able to perform different reductive pathways of the N-cycle, including both assimilatory processes, such as nitrate assimilation and N 2 fixation, and dissimilatory reactions, such as nitrate respiration and denitrification. However, nitrogen metabolism is much less known in archaea than in bacteria. The availability of the complete genome sequences of several members of the eury-and crenarchaeota has enabled new approaches to the understanding of archaeal physiology and biochemistry, including metabolic reactions involving nitrogen compounds. Comparative studies reveal that significant differences exist in the structure and regulation of some enzymes involved in nitrogen metabolism in archaea, giving rise to important conclusions and new perspectives regarding the evolution, function and physiological relevance of the different N-cycle processes. This review discusses the advances that have been made in understanding nitrate reduction and other aspects of the inorganic nitrogen metabolism in archaea.

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“…Organisms from all three domains of life are capable of carrying out this process. In addition to the experimentally characterized nitrite reductase (NirK) and nitrate reductase (NarGH) in H. marismortui (Yoshimatsu et al 2000(Yoshimatsu et al , 2002Ichiki et al 2001), the genome analysis identified at least four additional proteins with functions important for denitrification, viz. a nitrilase, a carbon-nitrogen hydrolase involved in reduction of organic nitrogen compounds and ammonia production; an ammonia-dependent NAD synthetase; a dissimilatory nitrate reductase; and an ammonium transporter.…”

Section: The Integrated Annotation and Network Discovery Approach Impmentioning

confidence: 99%

Genome sequence of Haloarcula marismortui: A halophilic archaeon from the Dead Sea

Baliga¹,

Bonneau²,

Facciotti³

et al. 2004

Genome Res.

278

210

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We report the complete sequence of the 4,274,642-bp genome of Haloarcula marismortui, a halophilic archaeal isolate from the Dead Sea. The genome is organized into nine circular replicons of varying G+C compositions ranging from 54% to 62%. Comparison of the genome architectures of Halobacterium sp. NRC-1 and H. marismortui suggests a common ancestor for the two organisms and a genome of significantly reduced size in the former. Both of these halophilic archaea use the same strategy of high surface negative charge of folded proteins as means to circumvent the salting-out phenomenon in a hypersaline cytoplasm. A multitiered annotation approach, including primary sequence similarities, protein family signatures, structure prediction, and a protein function association network, has assigned putative functions for at least 58% of the 4242 predicted proteins, a far larger number than is usually achieved in most newly sequenced microorganisms. Among these assigned functions were genes encoding six opsins, 19 MCP and/or HAMP domain signal transducers, and an unusually large number of environmental response regulators-nearly five times as many as those encoded in Halobacterium sp. NRC-1-suggesting H. marismortui is significantly more physiologically capable of exploiting diverse environments. In comparing the physiologies of the two halophilic archaea, in addition to the expected extensive similarity, we discovered several differences in their metabolic strategies and physiological responses such as distinct pathways for arginine breakdown in each halophile. Finally, as expected from the larger genome, H. marismortui encodes many more functions and seems to have fewer nutritional requirements for survival than does Halobacterium sp. NRC-1.

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Sequence and electron paramagnetic resonance analyses of nitrate reductase NarGH from a denitrifying halophilic euryarchaeote Haloarcula marismortui

Cited by 49 publications

References 37 publications

Anaerobic Metabolism in Haloferax Genus

Anaerobic Metabolism in Haloferax Genus

Nitrate reduction and the nitrogen cycle in archaea

Genome sequence of Haloarcula marismortui: A halophilic archaeon from the Dead Sea

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