Sequence and characteristics or IS<i>900</i>, an insertion element identified in a human Crohn's disease isolate or<i>Mycobacterium paratuberculosis</i>

Green, E.P.; Tizard, Mark; Moss, Maurice O.; Thompson, J.; Winterbourne, David J.; McFadden, Johnjoe; Hermon‐Taylor, John

doi:10.1093/nar/17.22.9063

Cited by 441 publications

(295 citation statements)

References 32 publications

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“…The site into which IS116 is inserted in pIJ702 (CATGGTCGGTCTCCTGGT) corresponds closely to the consensus sequence CATG( N),-,CPyCCTT proposed as the target site for IS900 insertion, based on analysis of three insertion events ( Fig. 4; Green et al, 1989). Moreover, the leftmost 6 and rightmost 17 residues of IS900 (Green et al, 1989) are closely similar to the corresponding segments of IS116.…”

Section: Similarities and Diflerences Between The Ends And Target Sitmentioning

confidence: 68%

“…Unusually, the insertion of IS116, IS900 or the mini-circle does not cause target site duplications ( Fig. 4; Green et al, 1989;Henderson et al, 1989) [the situation for IS110 is unclear because in the particular insertion event that was sequenced by Bruton & Chater (1987) the element had inserted into a run of seven C residues and was then flanked by runs of more than seven C residues]. The site into which IS116 is inserted in pIJ702 (CATGGTCGGTCTCCTGGT) corresponds closely to the consensus sequence CATG( N),-,CPyCCTT proposed as the target site for IS900 insertion, based on analysis of three insertion events ( Fig.…”

Section: Similarities and Diflerences Between The Ends And Target Sitmentioning

confidence: 99%

“…However, I S l l O (Chater et al, 1985;Bruton & Chater, 1987) and the 2.6 kb mini-circle (Lydiate et al, 1986;Henderson et al, 1989) from S. coelicolor and the recently discovered IS900 from Mycobacterium paratuberculosis (Green et al, 1989) have been found to form a 'family' of related elements based on similarity between amino acid sequences of their deduced gene products, despite the diversity of structure and host strains of these elements. The use of both IS110 and the mini-circle sequences to probe the DNA of a series of taxonomically varied streptomycetes revealed related elements in a minority of strains (Chater et al, 1985;Lydiate et al, 1986), but no hybridization to S. clavuligerus DNA.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of an insertion sequence, IS116, from Streptomyces clavuligerus and its relatedness to other transposable elements from actinomycetes

Leskiw

Mevarech

Barritt

et al. 1990

Journal of General Microbiology

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We have identified an insertion sequence, IS116, present in Streptomyces clavuligerus at one copy per genome. The element was discovered as a 1.4 kb insertion into the multicopy plasmid pIJ702 after propagation in S. clavuligerus. The nucleotide sequence of IS116 and the flanking sequences from pIJ702 have been determined. The junctions with pIJ702 show no target site duplication and there are no inverted repeats at the ends of the element. One putative coding open reading frame of 1197 bp was identified which would code for a protein product of 399 amino acids. This protein resembles deduced integrase/transposase proteins specified by three other transposable elements of actinomycetes: IS110 and the mini-circle from Stveptomyces coelicolor A3(2), and -most particularly -IS900 of Mycobacterium paratuberculosis. Two regions that are relatively conserved among these gene products show features found in similar positions in many reverse transcriptases. IS116 and IS900 are also closely similar in their general organization and (apparently) in their insertion site specificity, whereas IS110 and the mini-circle are quite different in these features.

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Section: Similarities and Diflerences Between The Ends And Target Sitmentioning

confidence: 68%

Section: Similarities and Diflerences Between The Ends And Target Sitmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Discovery of an insertion sequence, IS116, from Streptomyces clavuligerus and its relatedness to other transposable elements from actinomycetes

Leskiw

Mevarech

Barritt

et al. 1990

Journal of General Microbiology

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“…Map shares over 98% DNA homology with Maa and they have homologous major antigens [6,74]. However, phenotypic differences between these two subspecies, differentiated by the dependence of Map on mycobactin [165] and genetically by the presence of multiple copies of the Map specific insertion element IS900 [67], are important. These organisms induce, in a wide-ranging animal species, different pathologies associated to distinct clinical signs [28,82] and host adaptation [49,74,82].…”

Section: Physiological Characteristics For Host Adaptationmentioning

confidence: 99%

Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC)

Biet¹,

et al. 2005

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-Pathogens that are transmitted between the environment, wildlife, livestock and humans represent major challenges for the protection of human and domestic animal health, the economic sustainability of agriculture, and the conservation of wildlife. Among such pathogens, the genus Mycobacterium is well represented by M. bovis, the etiological agent of bovine tuberculosis, M. avium ssp. paratuberculosis (Map) the etiological agent of Johne disease, M. avium ssp. avium (Maa) and in a few common cases by other emergent environmental mycobacteria. Epidemiologic surveys performed in Europe, North America and New Zealand have demonstrated the existence and importance of environmental and wildlife reservoirs of mycobacterial infections that limit the attempts of disease control programmes. The aim of this review is to examine the zoonotic aspects of mycobacteria transmitted from the environment and wildlife. This work is focused on the species of two main groups of mycobacteria classified as important pathogens for humans and animals: first, M. bovis, the causative agent of bovine tuberculosis, which belongs to the M. tuberculosis complex and has a broad host range including wildlife, captive wildlife, domestic livestock, non-human primates and humans; the second group examined, is the M. avium-intracellulare complex (MAC) which includes M. avium ssp. avium causing major health problems in AIDS patients and M. avium ssp. paratuberculosis the etiological agent of Johne disease in cattle and identified in patients with Crohn disease. MAC agents, in addition to a broad host range, are environmental mycobacteria found in numerous biotopes including the soil, water, aerosols, protozoa, deep litter and fresh tropical vegetation. This review examines the possible reservoirs of these pathogens in the environment and in wildlife, their role as sources of infection in humans and animals and their health impact on humans. The possibilities of control and management programmes for these mycobacterial infections are examined with regards to the importance of their natural reservoirs.

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“…The growth of MAP was confirmed by polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) to demonstrate the presence of IS900 (Green et al, 1989;Whittington et al, 1998;Cousins et al, 1999). Faecal samples that were positive to all three tests done in series were considered positive.…”

Section: Faecal Sample Collection and Culturementioning

confidence: 99%

Association of farm soil characteristics with ovine Johne's disease in Australia

Dhand

Eppleston

Whittington

et al. 2009

Preventive Veterinary Medicine

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Speculation about the association of soil characteristics with the expression of ovine Johne's disease (OJD) prompted this cross-sectional study. We enrolled 92 sheep flocks in Australia during 2004-05 and in each enrolled flock collected pooled faecal samples from an identified cohort (group of same age and sex) of sheep and soil samples from the paddocks grazed by this cohort of sheep. Faecal pools were cultured to create three outcome variables: positive or negative status of faecal pools (pool OJD status, binary); the log number of viable Mycobacterium avium subsp. paratuberculosis (MAP) organisms per gram of faeces (log pool MAP number, continuous); and the prevalence of faecal shedders (cohort OJD prevalence level, ordinal: low <2%, medium 2-10% and high >10%). Separate statistical models were then developed to investigate the association between soil characteristics and each outcome variable.Sheep raised on soils with a higher percentage of organic carbon and clay had a higher OJD prevalence whereas, sheep grazing on soils with a higher content of sand and nitrogen had a lower OJD prevalence. Iron content of the soil was positively associated with OJD infection but the association between soil pH and OJD was inconclusive. Parent soil type, the only farm level factor, was not significant in any of the final models.Study results indicate a higher risk of OJD in sheep raised on soils with greater organic matter and clay content. We hypothesise that this is due to adsorption of MAP to clay and the 2 consequent retention of the bacteria in the topsoil, thus making them available in higher numbers to grazing sheep.

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Sequence and characteristics or IS900, an insertion element identified in a human Crohn's disease isolate orMycobacterium paratuberculosis

Cited by 441 publications

References 32 publications

Discovery of an insertion sequence, IS116, from Streptomyces clavuligerus and its relatedness to other transposable elements from actinomycetes

Discovery of an insertion sequence, IS116, from Streptomyces clavuligerus and its relatedness to other transposable elements from actinomycetes

Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC)

Association of farm soil characteristics with ovine Johne's disease in Australia

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