Sequence analysis of the ribosomal DNA ITS2 region in two Trichogramma species (Hymenoptera: Trichogrammatidae)

Ercan, Fahriye Sumer; Öztemiz, Sevcan; Tunçbilek, Aydin; Stouthamer, Richard

doi:10.2298/abs1104949e

Cited by 12 publications

(6 citation statements)

References 18 publications

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“…The ITS region of the highly repeated nrDNA gene family has widely proven useful and powerful in distinguishing among closely related species and even between cryptic species of Trichogramma (Ercan et al, 2011;Poorjavad et al, 2012). This is mostly due to its generally similar sequence pattern within species (Vogler & DeSalle, 1994).…”

Section: Discussionmentioning

confidence: 99%

DoesWolbachiainfection affect decision‐making in a parasitic wasp?

Farahani

Ashouri

Goldansaz

et al. 2015

Entomologia Exp Applicata

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Various physiological effects of Wolbachia infection have been reported in invertebrates, but the impact of this infection on behavior and the consequences of these behavioral modifications on fitness have rarely been studied. Here, we investigate the effect of Wolbachia infection on the estimation of host nutritive resource quality in a parasitoid wasp. We compare decision-making in uninfected and Wolbachia-infected strains of Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) on patches containing either fresh or old host eggs. For both strains, fresh eggs were better hosts than older eggs, but the difference was smaller for the infected strain than for the uninfected strain. Oviposition behavior of uninfected wasps followed the predictions of optimal foraging theory. They behaved differently toward high-vs. low-quality hosts, with more hosts visited and more ovipositions, fewer high-quality hosts used for feeding or superparasitism, and a sex ratio that was more biased toward females in patches containing high-quality hosts than in patches containing low-quality ones. Uninfected wasps also displayed shorter acceptance and rejection times in high-quality hosts than in hosts of lower quality. In contrast, infected wasps were less efficient in evaluating the nutritive quality of the host (fresh vs. old eggs) and had a reduced ability to discriminate between unparasitized and parasitized hosts. Furthermore, they needed more energy and therefore engaged in host feeding more often. This study highlights possible decision-making manipulation by Wolbachia, and we discuss its consequences for Wolbachia fitness.

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Section: Discussionmentioning

confidence: 99%

DoesWolbachiainfection affect decision‐making in a parasitic wasp?

Farahani

Ashouri

Goldansaz

et al. 2015

Entomologia Exp Applicata

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“…We first analyzed the phylogenetic backgrounds of the two strains. ITS-2 gene has widely proven to be a powerful tool in distinguishing among closely related Trichogramma species 42,63–66 . Its usefulness for species identification in Trichogramma is because: (1) smaller divergence within species than between species; (2) morphologically distinct cryptic species can be discriminated by sequence differences 42 .…”

Section: Discussionmentioning

confidence: 99%

Co-occurrence of thelytokous and bisexual Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) in a natural population

Liu

Zhou

Zhang

et al. 2019

Sci Rep

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Trichogramma dendrolimi is one of the most successful biocontrol agents in China. However, an inundative condition is necessary to obtain acceptable parasitism effect. A good solution to this is the application of its thelytokous counterparts which unfortunately are scarce in field. We here report the first case of a natural T. dendrolimi population in China comprising both bisexual wasps and an extremely low proportion of thelytokous wasps. These two forms of T. dendrolimi are phylogenetically related based on the reconstructions of ITS-2 and COI genes. Also, the phylogenetic results suggested a potentially Wolbachia-drived ITS-2 variation. The expression of thelytoky was hardly affected by temperature, which might help control Asian corn borer and Dendrolimus punctatus. Wolbachia are responsible for current thelytoky according to phylogenetic analyses, antibiotic treatment and introgression experiment. We also present the third case of paternal sex ratio chromosome that restrains the expansion of Wolbachia. Moreover, the low frequency of thelytoky may be common in natural populations. Consequently if for biological control it is determined that a thelytokous strain is to be preferred, then large number of field collected females should be set up as isofemale lines, to detect the rare thelytoky.

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“…rDNA genes or nucleotide regions have proven to be useful in taxonomic studies for various taxa and one of the most important target regions used in molecular analysis was ribosomal DNA which is relatively less changed and is composed of 28S D2 gene regions and internal spaces ITS2. The sequence analysis of ITS2 rDNA has been described in several studies as a tool for Trichogrammatidae identification as well (Kan et al 1996;Pinto et al 1997Pinto et al , 2002Sayed et al 2011;Sumer et al 2011). 28S rDNA coding rRNA is accepted as a highly conserved gene region during evolutionary processes, so it is used to construct phylogenetic trees especially for higher categories.…”

Section: Oligosita Balcluthae Viggiani Et Laudonia N Sp Figs 1mentioning

confidence: 99%

Description of a new species of Oligosita Walker (Chalcidoidea: Trichogrammatidae), egg parasitoid of Balclutha brevis Lindberg (Homoptera: Cicadellidae) living on Pennisetum setaceum, from Italy

Bella¹,

Cupani

D'Urso

et al. 2015

Zootaxa

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A new species of Oligosita Walker (Chalcidoidea: Trichogrammatidae), O. balcluthae Viggiani et Laudonia n. sp., is described as a parasitoid of the eggs of Balclutha brevis Lindberg (Homoptera: Cicadellidae) associated with crimson fountain grass, Pennisetum setaceum (Poaceae) in Italy. Morphological features and biology of the new species are discussed and illustrated. The 28S-D2 and ITS2 regions were successfully amplified and sequenced.

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Sequence analysis of the ribosomal DNA ITS2 region in two Trichogramma species (Hymenoptera: Trichogrammatidae)

Cited by 12 publications

References 18 publications

DoesWolbachiainfection affect decision‐making in a parasitic wasp?

DoesWolbachiainfection affect decision‐making in a parasitic wasp?

Co-occurrence of thelytokous and bisexual Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) in a natural population

Description of a new species of Oligosita Walker (Chalcidoidea: Trichogrammatidae), egg parasitoid of Balclutha brevis Lindberg (Homoptera: Cicadellidae) living on Pennisetum setaceum, from Italy

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