Sequence Analysis of the Complete Genome of an Iridovirus Isolated from the Tiger Frog

He, Jian; Lü, Ling; Deng, Min; He, Hui‐Chan; Weng, Shao P.; Wang, Xiao H.; Zhou, Song; Qin, Long; Wang, Xun Z.; Chan, Siu Ming

doi:10.1006/viro.2001.1245

Cited by 123 publications

(93 citation statements)

References 70 publications

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“…Previous studies of DNA polymerases of phycodnaviruses, iridoviruses, ascoviruses and an asfarvirus suggested these viruses were related and thus that they may have been derived from a common ancestral virus (He et al, 2002;Stasiak et al, 2000). The two viral types among these that appeared most closely related based on their DNA polymerases were ascoviruses and iridoviruses (He et al, 2002;Stasiak et al, 2000).…”

Section: Discussionmentioning

confidence: 93%

“…For example, algal viruses (family Phycodnaviridae), African swine fever virus (family Asfarviridae) and iridoviruses (family Iridoviridae) all produce large icosahedral virions containing an internal lipid membrane between the core and the capsid and a linear dsDNA genome (Dixon et al, 2000;Goorha & Murti, 1982;Heppel & Bethiaume, 1992;van Etten, 2000;Ward & Kalmakoff, 1991;Williams et al, 2000). Phylogenetic analyses of their DNA polymerase, major capsid protein and several other virally encoded structural proteins and enzymes suggest these viruses originated from a common ancestral icosahedral nucleocytoplasmic DNA virus (He et al, 2002;Iyer et al, 2001;Knopf, 1998;Stasiak et al, 2000), which also may be the evolutionary source of poxviruses (Iyer et al, 2001;Salas et al, 1999). There is little evidence, however, that two virus families that produce virions with markedly different structural properties are closely related and evolved from a common ancestor, or that one evolved from another.…”

Section: Introductionmentioning

confidence: 99%

“…There is little evidence, however, that two virus families that produce virions with markedly different structural properties are closely related and evolved from a common ancestor, or that one evolved from another. Recent studies of DNA polymerases of large dsDNA viruses indicate that ascoviruses and iridoviruses are closely related (He et al, 2002;Knopf, 1998;Stasiak et al, 2000). However, phylogenetic analysis of this protein alone is insufficient to infer the evolutionary relationship of these viruses, and specifically whether they descended from a common ancestor or one evolved from the other.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for the evolution of ascoviruses from iridoviruses

Stasiak

Renault

Demattei

et al. 2003

Journal of General Virology

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Ascoviruses (family Ascoviridae) are large, enveloped, double-stranded (ds)DNA viruses that attack lepidopteran larvae and pupae, and are unusual in that they are transmitted by parasitic wasps during oviposition. Previous comparisons of DNA polymerase sequences from vertebrate and invertebrate viruses suggested that ascoviruses are closely related to iridoviruses. This relationship was unexpected because these viruses differ markedly in virion symmetry, genome configuration and cellular pathology. Here we present evidence based on sequence comparisons and phylogenetic analyses of a greater range of ascovirus proteins and their homologues in other large dsDNA viruses that ascoviruses evolved from iridoviruses. Consensus trees for the major capsid protein, DNA polymerase, thymidine kinase and ATPase III from representative ascoviruses, algal viruses (family Phycodnaviridae), vertebrate and invertebrate iridoviruses (family Iridoviridae) and African swine fever virus (ASFV; family Asfarviridae) showed that ascovirus proteins clustered most closely with those of the lepidopteran iridovirus Chilo iridescent virus (CIV) (Invertebrate iridescent virus 6). Moreover, analysis of the presence or absence of homologues of an additional 50 proteins encoded in the genome of Spodoptera frugiperda ascovirus showed that about 40 % occurred in CIV, with lower percentages encoded by the genomes of, respectively, vertebrate iridoviruses, phycodnaviruses and ASFV. The occurrence of three of these genes in SfAV-1a but not CIV was indicative of the evolutionary differentiation of ascoviruses from invertebrate iridoviruses.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence for the evolution of ascoviruses from iridoviruses

Stasiak

Renault

Demattei

et al. 2003

Journal of General Virology

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“…Vertebrate IV genomes range from 105 kbp for tiger frog virus (17) to 186 kbp for lymphocystis disease virus, China strain (LCDV-C) (46). The Ranavirus and Megalocytivirus species have GϩC contents of approximately 50%, while the Lymphocystivirus species have GϩC contents of less than 30%.…”

mentioning

confidence: 99%

Genomic and Proteomic Analysis of Invertebrate Iridovirus Type 9

Wong

Young

Kleffmann

et al. 2011

J Virol

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Iridoviruses (IV) are nuclear cytoplasmic large DNA viruses that are receiving increasing attention as sublethal pathogens of a range of insects. Invertebrate iridovirus type 9 (IIV-9; Wiseana iridovirus) is a member of the major phylogenetic group of iridoviruses for which there is very limited genomic and proteomic information. The genome is 205,791 bp, has a G؉C content of 31%, and contains 191 predicted genes, with approximately 20% of its repeat sequences being located predominantly within coding regions. The repeated sequences include 11 proteins with helix-turn-helix motifs and genes encoding related tandem repeat amino acid sequences. Of the 191 proteins encoded by IIV-9, 108 are most closely related to orthologs in IIV-3 (Chloriridovirus genus), and 114 of the 126 IIV-3 genes have orthologs in IIV-9. In contrast, only 97 of 211 IIV-6 genes have orthologs in IIV-9. There is almost no conservation of gene order between IIV-3, IIV-6, and IIV-9. Phylogenetic analysis using a concatenated sequence of 26 core IV genes confirms that IIV-3 is more closely related to IIV-9 than to IIV-6, despite being from a different genus of the Iridoviridae. An interaction between IIV and small RNA regulatory systems is supported by the prediction of seven putative microRNA (miRNA) sequences combined with XRN exonuclease, RNase III, and double-stranded RNA binding activities encoded on the genome. Proteomic analysis of IIV-9 identified 64 proteins in the virus particle and, when combined with infected cell analysis, confirmed the expression of 94 viral proteins. This study provides the first full-genome and consequent proteomic analysis of group II IIV.

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“…Based on the Eighth Report of the International Committee on Taxonomy of Virus (ICTV), the family Iridoviridae has been subdivided into five genera: Iridovirus, Chloriridovirus, Ranavirus, Lymphocystisvirus, and Megalocytivirus [3]. To date, 12 genome sequences of iridoviruses including at least one from each genus have been completely sequenced and analysed [2,[4][5][6][7][8][9][10][11][12][13][14]. These analyses show that iridoviruses encode a number of cellular protein homologues that may be mostly involved in nucleic acid metabolism, such as ribonucleotide reductase (RR), thymidylate synthase (TS), deoxyuridine triphosphatase (DUT), and purine nucleoside phosphorylase (PNP) [15].…”

Section: Introductionmentioning

confidence: 99%

Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein

et al. 2009

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The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h postinfection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells.

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Sequence Analysis of the Complete Genome of an Iridovirus Isolated from the Tiger Frog

Cited by 123 publications

References 70 publications

Evidence for the evolution of ascoviruses from iridoviruses

Evidence for the evolution of ascoviruses from iridoviruses

Genomic and Proteomic Analysis of Invertebrate Iridovirus Type 9

Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein

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