Sequence analysis of serum paraoxonase 1 of bathyergidae family specific rats

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Objective: The balance between deaths and cellular life is regulated by B-cell lymphoma 2 (BCL-2)-associated X protein (BAX) an important proapoptotic components of BCL-2 family. With this initial point, the aim of this study was to determine a comparative composite based structure of BAX of Chinese liver fluke and different structural analysis. Methods:Protein amino acid of BAX of Chinese liver fluke mined from National Centre for Biotechnology Information (http://ncbi.nlm.nih.gov). Molecular model of BAX of Chinese liver fluke protein was generated by the comparative composite modeling tool Iterative Threading ASSEmbly Refinement suite. Afterward, I-TASSER generated molecular model was subjected to further structural improvements by energy minimization step. Distribution of negatively and positively charged amino acid over molecular modeled structure, distribution of secondary structural elements, and hydrophobicity molecular surface analysis was performed with the help of bioinformatical tools.Results: Analysis of Ramachandran plot created by PROCHECK tool is a consensus standard for validation purpose of protein structural modeling. Altogether 97.8% of the residues were detected in allowed and favored regions, which in turn validate the quality of generated protein structural model. Total negatively and positively charged residues within the BAX of Chinese liver fluke were 23 and 20, respectively. Chimera package-guided hydrophobicity molecular surface analysis illustrates that molecule specific hydrophobicity surface is exclusive to BAX protein molecule. Conclusion:Within the scope of this scientific investigation, we have successfully utilized molecular modeling approach to suggest the first molecular three-dimensional model structure of BAX of Chinese liver fluke. The synchronous balance between cellular deaths and cellular life is keeping up by BAX, an important pro-apoptotic family member of BCL-2 family. Consequently, it would be an exciting approach to resolve its structural characterization and molecular structure to propose mode of mechanism action.

Section: Resultsmentioning

confidence: 99%

The Biophysical Characteristics and Structural Exploration of Programmed Cell Death Regulator B-Cell Lymphoma 2-Associated X Protein of Chinese Liver Fluke (Clonorchis Sinensis)

Panda¹,

Prasad²,

Bag³

2017

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“…science, pharmaceutical science, and biological science [10][11][12][13][14][15][16][17]. In recent years, literatures have reported that hesperetin possesses the potential to behave as an effective inhibitor of thrombin-like serine proteases isolated from the venom of Crotalus simus snakes [18].…”

Section: Panda and Ehsanmentioning

confidence: 99%

Molecular Docking Studies of Snake Venom Serine Protease of Sharp-Nosed Pit Viper With Hesperetin

Panda¹,

Ehsan

2018

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Objective: The management of snake bite envenomation is a global challenge affecting millions of people. Immunotherapy is still regarded as the treatment of choice; however, their subsequent adverse effects restrict their potential use for therapy against snake venom poisoning. In recent years, more attention has been given to the exploration of indigenous medicinal plants for their outstanding benefits for the treatment of several diseases and disorders, including snake venom poisoning. Hesperetin is a naturally occurring compound derived from a flavanone glycoside, hesperidin and is obtained from various citrus fruits. It is known to possess significant inhibitory activity against snake venom serine proteases. The aim of our present study was to investigate the significant inhibitory action of hesperetin on thrombin-like serine protease from sharp-nosed pit viper (Deinagkistrodon acutus) snake venom. Methods:We have employed molecular docking analysis by implementing the state-of-the-art docking program to validate the hypothesis of the prospective inhibitory properties of hesperetin on thrombin-like serine proteases of snake venom. AutoDock 4.2, InterProSurf, MGLTools, and MTiAutoDock were utilized for the molecular docking analysis of thrombin-like serine protease obtained from the snake venom of sharp-nosed pit viper with the natural compound hesperetin. Results:The results generated from in silico based approach reveals the significant inhibitory role of hesperetin against thrombin-like snake venom proteases, which might lead to the drug designing of the inhibitors of snake venom serine proteases. Conclusion:The implementation of molecular docking analysis by the employment of state-of-the-art docking program supports the potential of inhibitory activity of naturally obtained hesperetin compound on thrombin-like serine proteases of snake venom. The generated in silico results suggests that the novel structure hesperetin -flavanone might act as a potent inhibitor of thrombin-like snake venom proteases, and unlocks the possibilities for designing drugs of the inhibitors of snake venom serine proteases. Moreover, the investigation of the novel compound obtained from natural sources for their inhibitory activity against snake venom serine proteases would lead to the discovery of newer inhibitory compound from a highly uninvestigated research arena.

“…While planning, we have to take into consideration the strategies and experiences of other countries to circumvent costly errors and to revise the work in generating and managing the national health information system. Fortunately, recent state of the art informatics research practices makes it accomplishable to address tangled research problems in biomedical sciences [29][30][31][32][33][34][35][36].…”

Section: Health Information System: An Overviewmentioning

confidence: 99%

The Role of Health Information System in Maternal and Child Healthcare Services

Chowdhury¹,

Kumari

Panda³

2017

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Health information system deals with any system that helps in capturing, storing, transmitting, and managing health-related information of an individual or to demonstrate the activities or organizations working within health-care sector. In the developing countries, maternal and child health is gaining concern due to increasing cases of morbidity and mortality. The disparities among the maternal, infant, and child health are a growing concern in India and are governed by various determinants such as socioeconomic status, literacy, quality of health care, discrimination, and biological and genetic factors. Accurate and reliable health information and data are the basis for decision-making across the health-care sector and are crucial for the development and implementation of health system policy by the policy-makers. Strict monitoring and evaluation of the present program design and its implementation is required at the microlevel to effectively utilize the resources for the improvement of maternal and child health. Our present article focuses on evaluating the coverage gap at the different levels for the provision of health-care facilities to maternal, neonatal, and child health, immunization, and treatment of poor children. Big data plays a major role in providing sound and reliable health-related information and also help in managing and recording structured and unstructured data. More concrete plans are required further to reduce the inequalities in health-care interventions for providing better maternal and child health-care services in our nation.