Sequence analysis of Ricinus communis small heat-shock protein (sHSP) subfamily and its role in abiotic stress responses

Neto, Valdir Gomes; Barbosa, Rhaissa R.; Carosio, Maria G.A.; Ferreira, Antônio G.; Fernandez, Luzimar Gonzaga; Castro, Renato Delmondez de; Ligterink, Wilco; Hilhorst, H.W.M.; Ribeiro, Paulo R.

doi:10.1016/j.indcrop.2020.112541

Cited by 16 publications

(8 citation statements)

References 60 publications

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“…The possible contribution of each individual metabolite was inferred by setting the variable importance in projection (VIP) threshold to 1 [22,23] . Seventeen metabolites showed VIP values greater than 1 and they are most likely responsible for the differences observed in the PLS‐DA plot ( Figure 1 b ).…”

Section: Resultsmentioning

confidence: 99%

HPLC/HR‐MS‐Based Metabolite Profiling and Chemometrics: A Powerful Approach to Identify Bioactive Compounds from Abarema cochliacarpos

Farias

Cerqueira

Colepicolo

et al. 2021

Chemistry & Biodiversity

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Despite its importance as a medicinal plant, there is a lack of studies that assessed the chemical composition of A. cochliacarpos extracts. Herein, we used a metabolite profiling approach and chemometrics as a powerful strategy to correlate the chemical composition with the antioxidant activity of A. cochliacarpos extracts. Extracts obtained with ethyl acetate showed greater antioxidant activity and higher total phenolic content than extracts obtained with hexane. The chemical composition was assessed by HPLC/HR‐MS and it encompassed fatty alcohols, terpenoids, phenolic derivatives, lipids, carotenoid‐like compounds, alkaloids, flavonoids, polyketides, and glycerophospholipids. Chemometrics successfully differentiated not only the chemical composition of extracts in response to the nature of the extraction solvent and the botanical part used during extraction but also it allowed us to associate the chemical composition with the antioxidant activity of the extracts, which might be particularly helpful for drug discovery and development programs.

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Section: Resultsmentioning

confidence: 99%

HPLC/HR‐MS‐Based Metabolite Profiling and Chemometrics: A Powerful Approach to Identify Bioactive Compounds from Abarema cochliacarpos

Farias

Cerqueira

Colepicolo

et al. 2021

Chemistry & Biodiversity

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“…The embryogenesis of soybean was reported to be accompanied with accumulation of specific HSP transcripts, and the levels of individual mRNAs positively correlated with seed longevity [ 14 ]. It was shown recently, that expression of 41 RcsHSP genes encoding cytoplasmic, mitochondrial and microsomal α-crystalline domain-containing HSPs, accompanied maturation of castor bean ( Ricinus communis) seeds [ 35 ]. Kaur et al reported an essential increase in the abundance of the OsHSP18.2 transcripts in rice seeds at the late maturation stage [ 31 ].…”

Section: Mechanisms Behind the Onset Of Desiccation Tolerance Durimentioning

confidence: 99%

Desiccation Tolerance as the Basis of Long-Term Seed Viability

Smolikova

Leonova

Vashurina

et al. 2020

IJMS

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Desiccation tolerance appeared as the key adaptation feature of photoautotrophic organisms for survival in terrestrial habitats. During the further evolution, vascular plants developed complex anatomy structures and molecular mechanisms to maintain the hydrated state of cell environment and sustain dehydration. However, the role of the genes encoding the mechanisms behind this adaptive feature of terrestrial plants changed with their evolution. Thus, in higher vascular plants it is restricted to protection of spores, seeds and pollen from dehydration, whereas the mature vegetative stages became sensitive to desiccation. During maturation, orthodox seeds lose up to 95% of water and successfully enter dormancy. This feature allows seeds maintaining their viability even under strongly fluctuating environmental conditions. The mechanisms behind the desiccation tolerance are activated at the late seed maturation stage and are associated with the accumulation of late embryogenesis abundant (LEA) proteins, small heat shock proteins (sHSP), non-reducing oligosaccharides, and antioxidants of different chemical nature. The main regulators of maturation and desiccation tolerance are abscisic acid and protein DOG1, which control the network of transcription factors, represented by LEC1, LEC2, FUS3, ABI3, ABI5, AGL67, PLATZ1, PLATZ2. This network is complemented by epigenetic regulation of gene expression via methylation of DNA, post-translational modifications of histones and chromatin remodeling. These fine regulatory mechanisms allow orthodox seeds maintaining desiccation tolerance during the whole period of germination up to the stage of radicle protrusion. This time point, in which seeds lose desiccation tolerance, is critical for the whole process of seed development.

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“…The embryogenesis of soybean was reported to be accompanied with accumulation of specific HSP transcripts, and the levels of individual mRNAs positively correlated with seed longevity [14]. It was shown recently, that expression of 41 RcsHSP genes encoding cytoplasmic, mitochondrial and microsomal αcrystalline domain-containing HSPs, accompanied maturation of castor bean (Ricinus communis) seeds [35]. Kaur et al reported an essential increase in the abundance of the OsHSP18.2 transcripts in rice seeds at the late maturation stage [31].…”

Section: Heat Shock Proteins (Hsps)mentioning

confidence: 99%

Desiccation Tolerance as The Basis of Long-Term Seed Viability

Smolikova¹,

Leonova²,

Vashurina³

et al. 2020

Preprint

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Desiccation tolerance appeared as the key adaptation feature of photoautotrophic organisms for survival in terrestrial habitats. During the further evolution, vascular plants developed complex anatomy structures and molecular mechanisms to maintain the hydrated state of cell environment, which essentially increased their ability to sustain water deficit and dehydration. However, the role of the genes encoding the mechanisms behind this adaptive feature in the higher vascular plants is restricted to the dehydration protection of spores, seeds and pollen, whereas the mature vegetative stages became sensitive to desiccation. During maturation, orthodox seeds lose up to 95% of their water and successfully enter dormancy. This feature allows seeds maintaining their viability even under strongly fluctuating environmental conditions. The mechanisms behind the desiccation tolerance are activated at the late seed maturation stage and are associated with the accumulation of late embryogenesis abundant proteins (LEA proteins), small heat shock proteins (sHSP), non-reducing oligosaccharides, and antioxidants of different chemical nature. The main regulators of maturation and desiccation tolerance onset are abscisic acid and protein DOG1, which control the network of transcription factors, among which are LEC1, LEC2, FUS3, ABI3, ABI5, AGL67, PLATZ1, PLATZ2. This network is complemented by epigenetic regulation of gene expression by methylation of DNA, post-translational modifications of histones and chromatin remodeling impact on seed desiccation tolerance and longevity. Moreover, orthodox seeds are able to maintain desiccation tolerance during germination up to the stage of radicle protrusion. This time point is critical in the process of seed development, as the seeds lose desiccation tolerance at this moment.

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Sequence analysis of Ricinus communis small heat-shock protein (sHSP) subfamily and its role in abiotic stress responses

Cited by 16 publications

References 60 publications

HPLC/HR‐MS‐Based Metabolite Profiling and Chemometrics: A Powerful Approach to Identify Bioactive Compounds from Abarema cochliacarpos

HPLC/HR‐MS‐Based Metabolite Profiling and Chemometrics: A Powerful Approach to Identify Bioactive Compounds from Abarema cochliacarpos

Desiccation Tolerance as the Basis of Long-Term Seed Viability

Desiccation Tolerance as The Basis of Long-Term Seed Viability

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