Sequence analysis of Pasteurella multocida major outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge

Luo, Yingchun; Zeng, Qiandon; Glisson, John R.; Jackwood, Mark W.; Cheng, I-Hsing N; Wang, Chinling

doi:10.1016/s0264-410x(98)00266-7

Cited by 41 publications

(29 citation statements)

References 31 publications

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“…Comparative analysis of the deduced amino acid sequences of the OmpH protein in somatic serotype strains of P. multocida has indicated that molecular mass heterogeneity is due to variation in the number of amino acids in two discrete hypervariable regions that are thought to correspond to external surface-exposed loops (Luo et al, 1999). Molecular mass heterogeneity also occurs in the corresponding P2 (OmpH) and P5 (OmpA) proteins of Haemophilus influenzae and is also due to differences in the size of hypervariable surface-exposed loop regions (Sikkema & Murphy, 1992;Duim et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…P. multocida expresses heat-modifiable (OmpA) and porin (OmpH) proteins on the cell surface (Lugtenberg et al, 1984;Luo et al, 1997Luo et al, , 1999, but very little is known about the precise roles of these proteins in pathogenesis. Heterogeneity of the OmpH protein in somatic serotype strains of P. multocida is due to the presence of hypervariable surface-exposed loop regions (Luo et al, 1999). In addition, the OmpH protein provides protection against P. multocida challenge in mice (Vasfi Marandi & Mittal, 1997) and chickens (Luo et al, 1999) and has potential as a vaccine candidate.…”

Section: Introductionmentioning

confidence: 99%

“…The heat-modifiable and porin proteins are important classes of OMPs that are surface-exposed and exhibit molecular mass and antigenic variation (Sikkema & Murphy, 1992;Duim et al, 1997). P. multocida expresses heat-modifiable (OmpA) and porin (OmpH) proteins on the cell surface (Lugtenberg et al, 1984;Luo et al, 1997Luo et al, , 1999, but very little is known about the precise roles of these proteins in pathogenesis. Heterogeneity of the OmpH protein in somatic serotype strains of P. multocida is due to the presence of hypervariable surface-exposed loop regions (Luo et al, 1999).…”

mentioning

confidence: 99%

“…Heterogeneity of the OmpH protein in somatic serotype strains of P. multocida is due to the presence of hypervariable surface-exposed loop regions (Luo et al, 1999). In addition, the OmpH protein provides protection against P. multocida challenge in mice (Vasfi Marandi & Mittal, 1997) and chickens (Luo et al, 1999) and has potential as a vaccine candidate. However, very little is known about OmpA and OmpH heterogeneity among porcine P. multocida strains associated with PAR and pneumonia.…”

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confidence: 99%

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Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

102

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One hundred and fifty-eight porcine strains of Pasteurella multocida, recovered primarily from cases of pneumonic pasteurellosis or progressive atrophic rhinitis (PAR) in England and Wales, were characterized by determination of their capsular types, presence or absence of the toxA gene and molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. Eighteen groups (clones) of strains were identified on the basis of specific combinations of capsular type, toxA status and outer-membrane protein (OMP)-type. The data provided evidence that different subpopulations of P. multocida are responsible for pneumonia and PAR in pigs. The majority (88 %) of cases of pneumonia were associated exclusively with non-toxigenic capsular type A strains of OMP-types 1.1, 2.1, 3.1 and 5.1 and capsular type D isolates of OMP-type 6.1. These strains were recovered from widespread geographical locations within England and Wales over a 12-year period and represented mostly single sporadic cases. The association of a small number of P. multocida variants with the majority of cases of porcine pneumonia suggests that these strains are not opportunistic pathogens of low virulence but represent primary pathogens with a relatively high degree of virulence. In contrast, the majority (76 %) of cases of PAR were associated with toxAcontaining capsular type D strains of OMP-type 4.1 and capsular type A and D strains of OMP-type 6.1. Toxigenic capsular type A strains associated with PAR and non-toxigenic capsular type A strains associated with pneumonia represent distinct subpopulations of P. multocida that can be differentiated by their OMP-types. The association of capsular types A and D with strains of the same OMP-types, and the absence and presence of the toxA gene in strains of the same OMP-types, suggest that horizontal transfer of capsular biosynthesis and toxA genes has occurred between strains representing certain subpopulations of P. multocida. INTRODUCTIONPasteurella multocida is the aetiological agent of progressive atrophic rhinitis (PAR) of swine and is of considerable economic importance to the pig-rearing industry throughout the world (Chanter & Rutter, 1989). PAR is characterized by atrophy of the nasal turbinate bones which, in severe cases, can lead to facial distortion. P. multocida strains associated with PAR usually produce a 145-kDa dermonecrotic toxin, which is encoded by the toxA gene (Lax et al., 1990;Buys et al., 1990). This toxin induces osteolysis in the turbinate bones and plays an important role in the pathogenesis of PAR (Kamp & Kimman, 1988). Toxigenic strains associated with PAR are most frequently of capsular type D (Eamens et al., 1988;Foged et al., 1988;Gardner et al., 1994;Lariviere et al., 1992), although toxigenic isolates of capsular type A can also be involved in the disease (Fussing et al., 1999;Sakano et al., 1992). Non-toxigenic P. multocida strains are not usually associated with PAR and confirmation of toxin production is important for the diagnosis and control of the disea...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

102

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“…Outro enfoque, relacionado com a caracterização antigênica foi realizado por Luo et al (1997Luo et al ( , 1999. Estes autores caracterizaram uma porina denominada proteína H ou porina H de 15 sorotipos somáticos de P. multocida.…”

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