Sequence analysis of genes mediating extended-spectrum beta-lactamase (ESBL) production in isolates of Enterobacteriaceae in a Lagos Teaching Hospital, Nigeria

Raji, Muhabat Adeola; Jamal, Wafaa; Ojemeh, Omoh; Rotimi, Vincent O.

doi:10.1186/s12879-015-1005-x

Cited by 31 publications

(25 citation statements)

References 31 publications

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“…First, the bla CTX-M-15 gene was the most commonly detected ESBL-encoding gene in our collection, as reported by other works conducted in North America, Europe, and South America [9]. Data from Malawi, Nigeria, and Tanzania are very similar with respectively 95.2%, 85.7%, and 100% of ESBL-producing E.coli belonging to the CTX-M-15 group [3,9,10].…”

Section: Discussionsupporting

confidence: 82%

Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti

Plantamura

Bousquet

Védy³

et al. 2019

J Infect Dev Ctries

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Introduction: While the molecular epidemiology of extended-spectrum-b-lactamase (ESBL)-producing E. coli is well known in Europe due to effective surveillance networks and substantial literature, data for Africa are less available, especially in Djibouti. Methodology: We studied 31 isolates of ESBL-producing E. coli from Djibouti and compared these molecular results with data available in Africa. Results: Susceptibility rates were 3.2% for ceftazidim, 48.4% for piperacillin-tazobactam, 90.3% for amikacine and 16.1% for ofloxacin. No isolate showed resistance to carbapenems or colistin. 30 E. coli (96.8%) were positive to blaCTX-M-15, 1 (3.2%) to blaCTX-M-14 and 10 (32.3%) to narrow-broad-spectrum blaTEM. No blaSHV were detected. Fluoroquinolone resistance analysis showed that 30 ofloxacin-resistant E. coli had the mutation Ser-83->Leu on the gyrA gene. 24 E. coli (77.4%) harboured the plasmid-borne aac(6 ')-Ib-cr gene. No E. coli carried the genes qnrA, qnrB and qepA. 10 isolates (32.3%) belonging to the ST131 clone. The plasmid incompatibility group most widely represented in our collection was IncFIA/IB/II. Conclusions: There is no major difference with African epidemiology. In particular, we notice the international diffusion of specific clonal group ST131.

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Section: Discussionsupporting

confidence: 82%

Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti

Plantamura

Bousquet

Védy³

et al. 2019

J Infect Dev Ctries

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“…isolated from chicken faecal samples [37] and ESBL production mediated by bla VEB in human clinical Providencia spp. and Citrobacter freundii isolates [36,38]. To our knowledge this study presents the first genomic characterization of ESBL production mediated by bla CTX-M-15 in human clinical isolates of Enterobacter hormaechei, Citrobacter werkmanii and Atlantibacter hermannii from Nigeria.…”

Section: Plos Onementioning

confidence: 90%

“…CTX-M-15 enzymes have been identified worldwide [34] including Nigeria in Klebsiella spp., Escherichia coli, Proteus spp., Pseudomonas aeruginosa and Enterobacter spp. [19,35,36]. There is limited data on the prevalence of ESBL-producing Enterobacteriaceae in Africa.…”

Section: Plos Onementioning

confidence: 99%

Whole genome sequencing of extended-spectrum β-lactamase genes in Enterobacteriaceae isolates from Nigeria

et al. 2020

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Extended Spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are of major concern as they are implicated in multidrug resistant nosocomial infections. They are listed on a recently published global priority list of antibiotic-resistant bacteria by the World Health Organization which raises concern in both healthcare and community settings. This study aimed at determining the frequency of ESBL genes in multidrug resistant human clinical Enterobacteriaceae isolates from Edo state Nigeria and to characterize the resistance mechanisms using whole genome sequencing. A total of 217 consecutive clinical isolates of Enterobacteriaceae, selection based on inclusion criteria, were collected from March-May 2015 from three medical microbiology laboratories of hospitals in Edo state Nigeria. All isolates were analyzed using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Antibiotic susceptibility testing was performed by Kirby-Bauer method and minimum inhibitory concentration (MIC) determination by E-test method. Double disc synergy test was used to screen for the production of ESBL. Whole genome sequencing (WGS) was performed for isolate characterization and identification of resistance determinants. Out of 217 consecutive clinical Enterobacteriaceae isolates, 148 (68.2%) were multi-drug resistant. Of these multi-drug resistant isolates, 60 (40.5%) were positive for the ESBL phenotypic test and carried ESBL genes. CTX-M-15 was the predominant ESBL found, among 93.3% (n = 56/60). Thirty-two plasmid incompatibility groups and 28 known and two new sequence types were identified among the ESBL isolates. The high occurrence of CTX-M-15 with associated resistant determinants in multidrug resistant Enterobacteriaceae harboring different plasmid incompatibility groups and sequence types calls for the need of continuous monitoring of this resistance threat to reduce its public health impact. To our knowledge, this study presents the first genomic characterization of ESBL production mediated by bla CTX-M-15 in human clinical isolates of Enterobacter hormaechei, Citrobacter werkmanii and Atlantibacter hermannii from Nigeria.

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“…A study from South Eastern Nigeria reported blaCTX-M-15 genes from urine, vaginal and wound swabs of out-patients younger than 30 years [28]. Two studies conducted in Western Nigeria by Olowe et al, [29] from blood, wound, HVS, and sputum samples and Raji et al, [30] from blood, Urine and wound samples revealed the isolates harbored blaCTX-M-1 and blaCTX-M-15 genes respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of invasiveEnterobacteriaceaefrom Pediatric Patients in Central and Northwestern Nigeria

Duru¹,

Olanipekun²,

Odili³

et al. 2020

Preprint

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BackgroundBacteremia is a leading cause of death in developing countries but etiologic evaluation is infrequent and empiric antibiotics are not evidence-based. Very little is known about the types of extended-spectrum β-lactamases (ESBL) in pediatric bacteremia patients in Nigeria. We evaluated the patterns of ESBL resistance in children enrolled into surveillance for community acquired bacteremic syndromes across health facilities in Central and Northwestern Nigeria. MethodBlood culture from suspected cases of sepsis from children age less than 5 years were processed using automated Bactec® incubator System from Sept 2008-Dec 2016. Enterobacteriacea were identified to the species level using Analytical Profile Index (API20E®) identification strip and antibiotic susceptibility profile was determined by the disc diffusion method. The multidrug resistant strains were then screened and confirmed for extended spectrum beta lactamase (ESBL) production by the combination disc method as recommended by Clinical and Laboratory Standard Institute (CLSI). Real time PCR was used to elucidate the genes responsible for ESBL production characterize the resistance genes ResultOf 21,000 children screened from Sept 2008-Dec 2016, 2,625(12.5%) were culture-positive. A total of 413 Enterobacteriaceae available for analysis were screened for ESBL. ESBL production was detected in 160/413(38.7%), comprising Klebsiella pneumoniae 105/160(65.6%), Enterobacter cloacae 21/160(13.1%), Escherichia coli 22/160(13.8%), Serratia species 4/160(2.5%), Pantoea species 7/160(4.4%) and Citrobacter species 1/160(0.6%). Of the 160 ESBL-producing isolates, high resistance rates were observed among ESBL-positive isolates for Ceftriaxone (92.3%), Aztreonam (96.8%), Cefpodoxime (96.25%), Cefotaxime (98.75%) and sulphamethoxazole-trimethoprim (90%), while 87.5 %, 90.63%, and 91.87% of the isolates were susceptible to Imipenem, Amikacin and Meropenem respectively. Frequently detected resistance genes were blaTEM 83.75%) (134/160), and, blaCTX-M 83.12% (133/160) followed by blaSHVgenes 66.25% (106/160). Co-existence of blaCTX-M, blaTEM and blaSHV was seen in 94/160 (58.8%), blaCTX-M and blaTEM in 118/160 (73.8%), blaTEM and blaSHV in 97/160 (60.6%) and blaCTX-M and blaSHV in 100/160 (62.5%) of isolates tested. ConclusionOur results indicate a high prevalence of ESBL resistance to commonly used antibiotics in Enterobacteriaceae isolates from bloodstream infections in children in this study. Careful choice of antibiotic treatment options and further studies to evaluate transmission dynamics of resistance genes could help in the reduction of ESBL resistance in these settings.

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Sequence analysis of genes mediating extended-spectrum beta-lactamase (ESBL) production in isolates of Enterobacteriaceae in a Lagos Teaching Hospital, Nigeria

Cited by 31 publications

References 31 publications

Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti

Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti

Whole genome sequencing of extended-spectrum β-lactamase genes in Enterobacteriaceae isolates from Nigeria

Molecular Characterization of invasiveEnterobacteriaceaefrom Pediatric Patients in Central and Northwestern Nigeria

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