Sequence Analysis of cytb Gene in Echinococcus granulosus from Western China

Zhong, Xianqiong; Wang, Ning; Hu, Dandan; Wang, Jiahai; Li, Tianyu; Gu, Xiaobin; Wang, Shuxian; Peng, Xuerong; Yang, Guangyou

doi:10.3347/kjp.2014.52.2.205

Cited by 22 publications

(14 citation statements)

References 22 publications

(36 reference statements)

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“…The cytochrome b ( Cyt b ) gene is an important part in the mtDNA genome for studying of species classification and detection of phylogenetic relations among diverse mammalian species where its nucleotide sequence is highly conserved [15], [9], [34]. In this work, 208-bp fragments from camel mtDNA cytochrome b were used for the identification of genetic diversity and conservation between 54 camels belonging to 6 camel breeds reared in Egypt named Baladi (Bal), Fallahi (Fal), Maghrabi (Mag), Mowaled (Mow), Sodany (Sod) and Somali (Som).…”

Section: Resultsmentioning

confidence: 99%

Cytochrome b conservation between six camel breeds reared in Egypt

Othman

El-Kader

Alam

et al. 2017

Journal of Genetic Engineering and Biotechnology

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This study was aimed to assess cytochrome b conservation in six breeds of camels reared in Egypt and to compare its sequence with those of other livestock species. The 208-bp fragments from camel mtDNA cyto b were amplified using PCR for 54 camels belonging to 6 camel breeds reared in Egypt. The alignment of camel cyto b sequences showed the presence of two polymorphic sites resulting in four haplotypes and their nucleotide sequences were submitted to GenBank under the accession numbers: KX909894–KX909897.The genetic distances between tested camel breeds were zero between Baladi, Fallahi and Maghrabi breeds whereas they were at low value between the other three breeds: Mowaled, Sodany and Somali. Neighbor-joining showed 4 branches; one of them include most of the tested animals and another one contains 2 Somali animals which is considered a specific haplotype for this breed. The other two branches are mixed between Sodani and Mowaled breeds.Neighbor-joining tree was constructed between cyto b sequences of our tested camels and their sequences from livestock species include Camelus dromedaries, Camelus bactrianus, Ovis aries, Capra hircus, Bubalus bubalis, Bos Taurus and Sus scrofa. The result confirmed that our camel breeds belong to Camelus dromedaries and are clearly separated from other species.It is concluded that cyto b sequence is highly conserved among all camel breeds reared in Egypt which belong to Camelus dromedaries in addition to the advantage of cyto b in differentiation between different livestock sources which enables it to widely use for the adulteration detection in mixed meat.

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Section: Resultsmentioning

confidence: 99%

Cytochrome b conservation between six camel breeds reared in Egypt

Othman

El-Kader

Alam

et al. 2017

Journal of Genetic Engineering and Biotechnology

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“…Differences in genetic variation as reflected in haplotype distribution among different populations of E. granulosus ( s.s .) in the Tibetan Plateau and other parts of China have also been observed [11, 31]. However, these differences could be a result of the short gene sequences analysed [39], the mitochondrial region investigated or the dissimilarities in the evolution rate of the various mitochondrial genes [13].…”

Section: Discussionmentioning

confidence: 99%

Genetic variation of Echinococcus spp. in yaks and sheep in the Tibet Autonomous Region of China based on mitochondrial DNA

Ohiolei¹,

Xia

Li³

et al. 2019

Parasites Vectors

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BackgroundCystic echinococcosis (CE) in humans and livestock is caused by Echinococcus granulosus (sensu lato). In China where CE is endemic, a number of studies have shown that Echinococcus granulosus (sensu stricto) is majorly responsible for CE. However, E. canadensis (G6) which is the second leading cause of CE is now being detected in most parts of the country. In this study, the species diversity and genetic variation of Echinococcus granulosus (s.l.) in four counties in Tibet Autonomous Region of China were investigated.MethodsInfection with Echinococcus granulosus (s.s.) in yaks and sheep was identified using NADH dehydrogenase subunit 1 and 5 (nad1 and nad5) mitochondrial genes while the genotype G6 of E. canadensis initially diagnosed with NADH dehydrogenase subunit 1 (nad1) was further confirmed by analysis of the complete mitochondrial genome and a phylogenetic network constructed based on the nad2 and nad5 genes.ResultsOut of 85 hydatid cyst samples collected from slaughtered sheep (n = 54) and yaks (n = 31), 83 were identified as E. granulosus (s.s.) G1 (n = 77), G3 (n = 6) and 2 were identified as E. canadensis G6. Analysis of the nad1/nad5 genes revealed 16/17 mutations with 9/14 parsimony informative sites resulting in 15/14 haplotypes, respectively. Haplotype diversity (Hd) and nucleotide diversity (π) of E. granulosus (s.s.) population were 0.650 and 0.00127 for nad1 and 0.782 and 0.00306 for nad5, respectively, with an overall negative Tajima’s D and Fu’s Fs. A low FST indicated no genetic difference between isolates from sheep and yaks.ConclusionPockets of infection with E. canadensis (G6, G7, G8 and G10) have been previously reported in sheep, goats, yaks and/or humans in different parts of China. While the G6 genotype has been previously reported in sheep in the Tibet Autonomous Region, the detection in a yak in the present study represents the first to the best of our knowledge. Therefore, we recommend future surveys and control efforts to comprehensively investigate other potential intermediate hosts for the prevalence and genetic diversity of the E. canadensis group (G6, G7, G8 and G10) across the country and their inclusion into the existing CE control programme.

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“…A number of mitochondrial and nuclear fragments have been used as markers to investigate the genotypes, or the genetic structures, of E. granulosus in southwestern China, including cox1, nad1, nad2, cytb, atp6, 12S rRNA, and 16S rRNA (Ma et al, 2008;Nakao et al, 2010a;Ma et al, 2012;Wang et al, 2013;Yan et al, 2013;Wang et al, 2014a;Zhong et al, 2014). However, markers of different lengths are not appropriate for the comparison of genetic structures in different regions, and gene fragments may not be representative because every fragment on a gene is unique in terms of its conservation characteristics.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Echinococcus granulosus on the Tibetan Plateau using mitochondrial DNA markers

Hu¹,

Song²,

Wang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Cystic echinococcosis (CE) is an important worldwide zoonotic disease that causes large economic losses and human suffering. Echinococcus granulosus, the causative agent of CE, exhibits different genotypes in different locations. In order to identify its genotypes and analyze its genetic structure on the Tibetan Plateau, we collected 72 hydatid cysts from different intermediate hosts and amplified and sequenced their mitochondrial cytochrome c oxidase subunit 2 (cox2) genes. Seventy isolates were identified as the E. granulosus G1 genotype, while two isolates belonged to the G6 genotype. There were 18 haplotypes among the 70 E. granulosus isolates, which exhibited a star-like network pattern and shared a common haplotype (H 1 ). There was little difference between geographical sub-populations. Our results suggest that a recent E. granulosus population expansion occurred 13915-13923 (2015) on the Tibetan Plateau, suggesting that E. granulosus was introduced into China. This study increases the basic molecular data needed for the molecular diagnosis, epidemiology, prevention, and control of Echinococcus diseases.

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Sequence Analysis of cytb Gene in Echinococcus granulosus from Western China

Cited by 22 publications

References 22 publications

Cytochrome b conservation between six camel breeds reared in Egypt

Cytochrome b conservation between six camel breeds reared in Egypt

Genetic variation of Echinococcus spp. in yaks and sheep in the Tibet Autonomous Region of China based on mitochondrial DNA

Molecular identification of Echinococcus granulosus on the Tibetan Plateau using mitochondrial DNA markers

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