Sequence analysis of a functional polymerase (L) gene of bovine respiratory syncytial virus: determination of minimal trans-acting requirements for RNA replication.

Yunus, Abdul S.; Collins, Peter L.; Samal, Siba K.

doi:10.1099/0022-1317-79-9-2231

Cited by 15 publications

(20 citation statements)

References 33 publications

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“…The P protein has 241 AA [2] and appears to act as a chaperone for soluble N and is implicated as a regulation factor for viral transcription and replication. The polymerase L of BRSV has a size of 2162 AA [171] and is an RNA-dependant RNA polymerase. This protein is responsible for the viral transcription and replication [171].…”

Section: Nucleocapsid Proteinsmentioning

confidence: 99%

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Bovine respiratory syncytial virus infection

Valarcher¹,

2007

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-Bovine respiratory syncytial virus (BRSV) belongs to the pneumovirus genus within the family Paramyxoviridae and is a major cause of respiratory disease in young calves. BRSV is enveloped and contains a negative sense, single-stranded RNA genome encoding 11 proteins. The virus replicates predominantly in ciliated respiratory epithelial cells but also in type II pneumocytes. It appears to cause little or no cytopathology in ciliated epithelial cell cultures in vitro, suggesting that much of the pathology is due to the host's response to virus infection. RSV infection induces an array of pro-inflammatory chemokines and cytokines that recruit neutrophils, macrophages and lymphocytes to the respiratory tract resulting in respiratory disease. Although the mechanisms responsible for induction of these chemokines and cytokines are unclear, studies on the closely related human (H)RSV suggest that activation of NF-κB via TLR4 and TLR3 signalling pathways is involved. An understanding of the mechanisms by which BRSV is able to establish infection and induce an inflammatory response has been facilitated by advances in reverse genetics, which have enabled manipulation of the virus genome. These studies have demonstrated an important role for the non-structural proteins in anti-interferon activity, a role for a virokinin, released during proteolytic cleavage of the fusion protein, in the inflammatory response and a role for the SH and the secreted form of the G protein in establishing pulmonary infection. Knowledge gained from these studies has also provided the opportunity to develop safe, stable, live attenuated virus vaccine candidates.

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Section: Nucleocapsid Proteinsmentioning

confidence: 99%

“…The polymerase L of BRSV has a size of 2162 AA [171] and is an RNA-dependant RNA polymerase. This protein is responsible for the viral transcription and replication [171].…”

Section: Nucleocapsid Proteinsmentioning

confidence: 99%

Bovine respiratory syncytial virus infection

Valarcher¹,

2007

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“…A fosfoproteína P atua como uma chaperonina para a forma solúvel da proteína N [79-81, 97,142]. A proteína P ainda serve como um co-fator para a polimerase viral (L), após a sua fosforilação [79,80,142,183]. A fosforilação da proteína P se deve ao alto conteúdo de resíduos de serina e treonina, que constituem, aproximadamente, 17% dos 241 aminoácidos da mesma [80].…”

Section: Proteínas Viraisunclassified

“…A fosforilação da proteína P se deve ao alto conteúdo de resíduos de serina e treonina, que constituem, aproximadamente, 17% dos 241 aminoácidos da mesma [80]. Na ausência de fosforilação da proteína P, a polimerase produz apenas uma série de oligonucleotídeos a partir da extremidade 3' do genoma, o que sugere que uma proteína P funcional é necessária para converter a polimerase viral nascente em um complexo estável [81, 183,185].…”

Section: Proteínas Viraisunclassified

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Vírus respiratório sincicial bovino

Spilki

Arns

2018

Acta Scientiae. Vet.

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RESUMOO Vírus Respiratório Sincicial Bovino (BRSV) é uma causa importante de doença respiratória, principalmente em bovinos jovens, caracterizada por pneumonia intersticial. A distribuição das infecções pelo BRSV é mundial e o vírus foi identificado em diferentes episódios no Brasil. O vírus pertence ao gênero Pneumovirus da família Paramyxoviridae e o seu genoma RNA não-segmentado de sentido negativo codifica para 10 proteínas. As proteínas de fusão (F) e de adesão (G) constituem os principais alvos para o sistema imune do hospedeiro. Esse fato deve estar relacionado com a grande variabilidade destas proteínas e de seus genes correspondentes, conforme determinado tanto por anticorpos monoclonais quanto por análise filogenética com base em seqüências de nucleotídeos. A transmissão, resposta imune e patogenia das infecções pelo BRSV, foram estudadas no hospedeiro natural sob condições naturais e experimentais; todavia, alguns aspectos da imunobiologia da infecção são ainda pouco conhecidos. O uso de modelos experimentais, especialmente roedores, pode fornecer novas informações sobre esse assunto. O estado atual do diagnóstico laboratorial das infecções e as medidas de controle para prevenir surtos de BRSV também são apresentados e discutidos.Descritores: vírus respiratório sincicial bovino, BRSV, doenças de bovinos. ABSTRACTBovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease, mainly in young cattle, characterized by interstitial pneumonia. Distribution of BRSV infections is worldwide and the virus has been identified in different episodes in Brazil. The virus belongs to the genus Pneumovirus of the Paramyxoviridae family and its negative sense non-segmented RNA genome encodes 10 proteins. Fusion (F) and attachment (G) proteins constitute the main target for immune responses of the host. This fact may be related to the great variability of these proteins and its corresponding genes among field isolates, as determined by both monoclonal antibodies and phylogenetic analysis using nucleotide sequences. Transmission, immune responses and pathogenesis of BRSV infections were studied in the natural host under natural and experimental conditions; however, some aspects regarding the immunobiology of infection are yet poorly understood. The use of experimental models of infection, especially rodents, may provide new information on these subjects. The present status on laboratory diagnosis of infections and control measures to prevent BRSV outbreaks are also presented and discussed.

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Mutational Analysis of the Bovine Respiratory Syncytial Virus Nucleocapsid Protein Using a Minigenome System: Mutations That Affect Encapsidation, RNA Synthesis, and Interaction with the Phosphoprotein

et al. 2000

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The nucleocapsid (N) protein of bovine respiratory syncytial virus (BRSV) is a multifunctional protein that plays a central role in transcription and replication of viral genomic RNA. To investigate the domains and specific residues involved in different N activities, we generated a total of 27 deletion and 12 point mutants of the N protein. These mutants were characterized using an intracellular BRSV-CAT minigenome replication system for the ability to (1) direct minigenome RNA synthesis, (2) direct minigenome encapsidation, and (3) form a complex with the phosphoprotein (P). The mutations tested were defective in synthesis of RNA from the BRSV-CAT minigenome template with the exception of the following: a deletion involving the first N-terminal amino acid and mutations involving conservative substitution at the second amino acid and at certain internal cysteine residues. Micrococcal nuclease enzyme protection assays showed that mutations involving amino acids 1-364 of the 391-amino-acid N protein prevented minigenome encapsidation. Thus the BRSV N protein has a C-terminal, 27-amino-acid tail that is not required for encapsidation. Interestingly, two of the mutations that ablated encapsidation did not greatly affect RNA synthesis; the mutant involving deletion of the N-terminal amino acid and the mutant involving a substitution at position 2. This finding indicates that the formation of a nucleocapsid sufficient to protect the RNA from nuclease is not required for template function. Coimmunoprecipitation of N and P using N- or P-specific antiserum revealed two regions of the N protein that are important for association with the P protein: a central portion of 244-290 amino acids and a C-terminal portion of 338-364 amino acids.

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Sequence analysis of a functional polymerase (L) gene of bovine respiratory syncytial virus: determination of minimal trans-acting requirements for RNA replication.

Cited by 15 publications

References 33 publications

Bovine respiratory syncytial virus infection

Bovine respiratory syncytial virus infection

Vírus respiratório sincicial bovino

Mutational Analysis of the Bovine Respiratory Syncytial Virus Nucleocapsid Protein Using a Minigenome System: Mutations That Affect Encapsidation, RNA Synthesis, and Interaction with the Phosphoprotein

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