Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

Mahmoodi, Mohammad; Afshari, Kian Pahlevan; Seyedabadi, Hamid Reza; Aboozari, Mehran

doi:10.7904/2068-4738-ix(18)-5

Cited by 10 publications

(5 citation statements)

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“…the PCR amplifications of mitochondrial 16S rRNA followed by sequencing and analysis showed to be very efficient for identification of species origin of species [21] . The 12S rRNA and 16S rRNA gene sequences of animals reveal the fitting level of interspecific variation but the great level of intraspecific homogeneity [28] .…”

Section: Resultsmentioning

confidence: 99%

“…Mitochondrial DNA typing founded on sequences of the control region otherwise filled genomic sequence is used to examine a variation of forensic mtDNA profiling methods used for human proof of identity and present their use in the chief cases of human identification from non-human [3][4][5] . Mitochondrial markers that are used for species identification are as follows: cytb gene, cytochrome c oxidase subunit I gene, 12S and 16S rRNA segment and control region in wildlife [6][7][8] . A short fragment of the 12S rDNA was employed for DNA amplification leading to species identification.…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial 16S rRNA gene-dependent Blood typing as a Forensic Tool

2020

IJFMT

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Background: Mitochondrial DNA is an important tool for human identification and is used to differentiate between human and animal blood at the crime scene, because in extreme conditions nuclear DNA is severely destroyed while Mitochondrial DNA contains multiple copies in per cell as well as resists harsh and more stable conditions. Methodology: Seventy-two blood samples were collected from human (Homo sapiens), sheep (Ovis aries), goat (Capra hircus) and cow (Bos taurus) (Eighteen blood samples for each). All blood samples were withdrawn by technician and 5ml were aspirated using aseptic technique and transferred to EDTA-Na2 tube and mixed well and stored in refrigerator. The collection takes 2 weeks (15th May 2019 to 30th May 2019). All samples were collected from Al-Diwanyia city. Results: The results of PCR reveal that, the primer pairs were specific and non-specific products not appear for all samples. The amplification of Homo sapiens mitochondrial DNA with primer pairs of other (Ovis aries, Capra hircus and Bos taurus) and amplification of each with primers pair of another genus gave negative results and this a primary evidence for primer pairs specificity. The amplicon of 16S rRNA gene of Homo sapiens were 1200bp ,Ovis aries were 1060bp ,Capra hircus were 820bp , and Bos taurus were 1300bp. The sequencing revealed that no crossreactivity of designed primer pairs and the PCR assay based on the designed primer pairs will be simple, fast, sensitive, specific, and cost-effective. Conclusion: Sensitivity, specificity and accuracy of the designed species specific primer pairs and applicability of the designed primer pairs in forensics to investigate blood sports or evidence belonging for human, sheep, goat and cow.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mitochondrial 16S rRNA gene-dependent Blood typing as a Forensic Tool

2020

IJFMT

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“…After the UGENE process, a dataset is created in the Microsoft Word application and combined with comparison data obtained from GenBank. Hemocyanin sequence profile analysis using BioEdit software for alignment and analyzed nucleotide base mutations and amino acid changes (Mahmoodi et al, 2018) from L. vannamei hemocyanin sequences. Data were compared with 3 L. vannamei hemocyanin sequences and 3 Penaeus monodon hemocyanin sequences from the National Center of Biotechnology Information (NCBI) Gen Bank.…”

Section: Discussionmentioning

confidence: 99%

Identification and Molecular Analysis of Hemocyanin as a Body Resistance Gene in Litopenaeus vannamei

Amanda,

Kilawati,

Maftuch

2024

jppipa, pendidikan ipa, fisika, biologi, kimia

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Production of white shrimp (Litopenaeus vannamei) tends to decrease every year caused by diseases, one of which is White Feces Disease (WFD). Genetically, monitoring has been carried out on the parent characters of SPF (Specific Pathogen Free) shrimp imported from Hawaii and Florida with low genetic variation. If there is a threat such as disease caused by bacteria and the gene that codes for disease control or the body's resistance gene is missing, it allows the individual to become susceptible to disease. Gene that codes for body resistance in white shrimp is hemocyanin. Hemocyanin gene studies are needed as a first step in controlling disease in white shrimp. Method used is a descriptive method. Procedures carried out are sample collection, detection of WFD, molecular analysis of hemocyanin and data analysis. Results showed that healthy shrimp had hemocyanin while shrimp with WFD had very little or no amount of hemocyanin. Hemocyanin gene profile in healthy shrimp was also analyzed and there were 4 nucleotide change made changes to 4 amino acid. Changes in amino acids affect the stability of hemocyanin and cause polymorphism of hemocyanin. Phylogenetic analysis showed dendogram split into two clades between L. vannamei and P. monodon.

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“…Complex formation bioreactions are equilibrium bioreactions (as well as most of the bioreactions chemical ones), and the shift of balance in one sense or another is evidence of greater stability or greater small complexes [29][30][31]. The stability of the complexes depends on the nature of the complex generating ion, or some its properties (electronic structure, charge, ionic radius, etc.…”

Section: The Role Of Colloidal Solutions In Biochemical Analysis Coamentioning

confidence: 99%

Interdisciplinary Character of Biochemistry and Analytical Biochemistry

Butnariu¹,

Sarac²

2018

Biochem Anal Biochem

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Biochemistry is a modern frontier science with an interdisciplinary character. Biochemistry has close links with organic chemistry, which provides support for knowing the structure and properties of biomolecules. Analytical chemistry, colloidal chemistry and chelates complexes provide biochemistry with methods of identifying, separating and characterizing biomolecules as well as kinetic and thermodynamic interpretation of biochemical reactions. Biochemical processes are carried out at cellular structures, so biochemistry interferes with molecular biology first and with morphology, which can also be approached from the aspect of structural element chemistry. Physiology raises a higher level of knowledge of the laws of life phenomena, and modern genetics has in fact become biochemical genetics, because it has created the basis for the codification of the hereditary message and has established relations between nucleic acids and protein biosynthesis as a premise of self-reproduction of living organisms.

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Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

Abstract: Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

Cited by 10 publications

References 0 publications

Mitochondrial 16S rRNA gene-dependent Blood typing as a Forensic Tool

Mitochondrial 16S rRNA gene-dependent Blood typing as a Forensic Tool

Identification and Molecular Analysis of Hemocyanin as a Body Resistance Gene in Litopenaeus vannamei

Interdisciplinary Character of Biochemistry and Analytical Biochemistry

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