Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

Mahmoodi, Mohammad; Afshari, Kian Pahlevan; Seyedabadi, Hamid Reza; Aboozari, Mehran

doi:10.4081/vsd.2018.7855

Cited by 3 publications

(4 citation statements)

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“…The PCR amplifications of mitochondrial 16S rRNA followed by sequencing and analysis were demonstrated to be very efficient for identification of species origin [21]. The 12S rRNA and 16S rRNA gene sequences of animals reveal the fitting level of interspecific variation but the great level of intraspecific homogeneity [7]. The results showed no crossreactivity of designed primer pairs and the PCR assay based on the designed primer pairs will be simple, fast, sensitive, specific, and cost-effective.…”

Section: Resultsmentioning

confidence: 97%

“…Mitochondrial DNA typing founded on sequences of the control region otherwise filled genomic sequence is used to examine a variation of forensic mtDNA profiling methods used for human proof of identity and present their use in the chief cases of human identification from non-human [3][4][5]. Mitochondrial markers that are used for species identification are as follows: cytb gene, cytochrome c oxidase subunit I gene, 12S and 16S rRNA segment, and control region in wildlife [6][7][8]. A short fragment of the 12S rDNA was employed for DNA amplification leading to species identification.…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial 16S rRNA Gene-Dependent Blood Typing as a Forensic Tool

Al-Dahmoshi¹,

Al-Nayili²

2021

Forensic Analysis - Scientific and Medical Techniques and Evidence Under the Microscope

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Mitochondrial DNA is an important tool for human identification and is used to differentiate between human and animal blood at the crime scene, because in extreme conditions nuclear DNA is severely destroyed while Mitochondrial DNA contains multiple copies (200–2000) per cell and resists harsh and more stable conditions. Seventy-two blood samples were collected from humans (Homo sapiens), sheep (Ovis aries), goats (Capra hircus), and cows (Bos taurus) (18 blood samples for each). All blood samples were withdrawn by a technician and 5 ml were aspirated using an aseptic technique and transferred to EDTA-Na2 tubes. They were mixed well and stored in a refrigerator. The collection took 2 weeks (May 15, 2019–May 30, 2019). All samples were collected from Al-Diwanyia city. The results of PCR testing revealed that the primer pairs were specific and non-specific products did not appear for all samples. The amplification of Homo sapiens mitochondrial DNA with primer pairs of other (Ovis aries, Capra hircus, and Bos taurus) and amplification of each with primer pairs of another genus gave negative results, and this is primary evidence for primer pair specificity. The amplicon of 16S rRNA gene of Homo sapiens was 1200 bp, Ovis aries was 1060 bp, Capra hircus was 820 bp, and Bos taurus was 1300 bp. The sequencing revealed that no cross-reactivity of designed primer pairs and the PCR assay based on the designed primer pairs will be simple, fast, sensitive, specific, and cost-effective. There is sensitivity, specificity, and accuracy in the designed species-specific primer pairs and applicability of the designed primer pairs in forensics to investigate blood spots or evidence belonging for human, sheep, goat, and cow.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Mitochondrial 16S rRNA Gene-Dependent Blood Typing as a Forensic Tool

Al-Dahmoshi¹,

Al-Nayili²

2021

Forensic Analysis - Scientific and Medical Techniques and Evidence Under the Microscope

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“…By affecting the hypothalamus (the area of the brain responsible for regulating food intake), MSG can lead to obesity. 1,[35][36][37] By affecting the nervous transmission pathways from the brain to muscle cells in the joints or muscles, MSG can produce or aggravate neurological diseases such as Alzheimer's, arthritis or fibromyalgia. 3,38,39 Researchers have found that many cancers have MSG receptors.…”

Section: Free Glutamate and Bound Glutamatementioning

confidence: 99%

What is sodium glutamate and what effects it has on health

Butnariu¹,

Sărac²

2019

JABB

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Sodium glutamate or monosodium glutamate is a substance found naturally in certain foods, but it is a compound used in the food industry to enhance the flavor. The compound is a non-essential amino acid, which is contained in tomatoes, milk, mushrooms, fish or cheese. In the food industry, sodium glutamate is also known as MSG or E621. Its role is to enhance the flavor of the dishes, as it has the chemical ability to enhance the flavor. In fact, MSG itself has no taste, but it is activated when it is combined with spices or flavored foods.

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“…It is widely used to differentiate between human from nonhuman species. Genotyping using 12S rRNA gene provide cost and time effective results, and yielded information not only for species identification but also for individualization [7,8] . The current study aimed to showed the validity of designed 12S rRNA species specific primer pairs for human and nonhuman animal blood typing as a forensic tool.…”

Section: Introductionmentioning

confidence: 99%

Forensic Human and Non-human Blood Discrimination using Mitochondrial 12S rRNA gene

2020

IJFMT

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Background:The blood samples of spots typing regards most important step in forensic analysis.Determining the blood affiliation as human on non-human is not enough as a forensic tool leading to the truth . The mitochondrial 12S rRNA gene are suitable for taxonomy and species identification, especially for the discrimination of even closely related species. The current study aimed to showed the validity of designed 12S rRNA species specific primer pairs for human and nonhuman animal blood typing as a forensic tool. Methodology: Seventy two blood samples were collected from Homo sapiens, Ovis aries, Capra hircus and Bos taurus (18 blood samples from each). Five milliliter were withdrawn and placed in EDTAtube and stored in refrigerator for further processing. Four sets of species specific primer pairs targeting mitochondrial 12S rRNA were designed and checked. PCR and sequencing were performed and sequences were analyzed and register in GenBank. Results: the results revealed that, the amplicon of 12S rRNA gene of Homo sapiens were 800bp, Ovis aries were 560bp, Capra hircus were 460bp, and Bos taurus were 600bp The first confirmatory test for validity and specificity of designed primer pairs is no amplification for intraspecies primer pairs (i.e. using homo12S rRNA primer pairs to amplify Ovis aries, Capra hircus, and Bos taurus 12S rRNA will not give product and vice versa). The second confirmatory test is the results of sequencing. The identity percentage and alignment of sequences results of amplified 12S rRNA gene of homo sapiens, Ovis aries, Capra hircus and Bos taurus revealed that, the similarity percentage ranged from 98.03% to 100%.Conclusion: The current study conclude that, validity and accuracy of designed 12S rRNA species specific primer pairs for human and nonhuman animal blood typing as a forensic tool and there is no intraspecies cross amplification.

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Sequence analysis of 12S rRNA and 16S rRNA mitochondrial genes in Iranian Afshari sheep

Cited by 3 publications

References 0 publications

Mitochondrial 16S rRNA Gene-Dependent Blood Typing as a Forensic Tool

Mitochondrial 16S rRNA Gene-Dependent Blood Typing as a Forensic Tool

What is sodium glutamate and what effects it has on health

Forensic Human and Non-human Blood Discrimination using Mitochondrial 12S rRNA gene

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