Sequence analysis in partial genes of five isolates of Angiostrongylus cantonensis from Taiwan and biological comparison in infectivity and pathogenicity between two strains

Lee, June-Der; Chung, Li-Yu; Wang, Lian-Chen; Lin, Rong-Jyh; Wang, Jhi-Joung; Tu, Hung-Pin; Wu, Zhongdao; Yen, Chuan-Min

doi:10.1016/j.actatropica.2014.01.010

Cited by 21 publications

(18 citation statements)

References 44 publications

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“…The most important biological differences observed between these haplotypes were the total number of L1 larvae eliminated in faeces and the parasitic burden recovered, which was 60% for ac9 haplotype and approximately 40% for ac8. These data corroborate the findings with R. norvegicus specimens infected with different Taiwanese strains of A. cantonensis , which showed adult recovery rates varying from 38-58% ( Lee et al 2014 ). In contrast, Au and Ko (1979) observed mean recovery rate of 82% after a small primary infection with A. cantonensis strain from Hong Kong, which was higher than that observed in the present study.…”

Section: Discussionsupporting

confidence: 89%

Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles

Monte

Gentile

Garcia

et al. 2014

Mem. Inst. Oswaldo Cruz

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Angiostrongylus cantonensis is the etiologic agent of eosinophilic meningoencephalitis in humans. Cases have been recorded in many parts of the world, including Brazil. The aim of this study was to compare the differences in the biology and morphology of two different Brazilian haplotypes of A. : ac8 and ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes. The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The morphometric analysis showed that male and female specimens isolated from ac8 haplotype were significantly larger with respect to body length, oesophagus length, spicule length (male) and distance from the anus to the rear end (female) compared to specimens from ac9. The morphological analysis by light microscopy showed little variation in the level of bifurcations at the lateral rays in the right lobe of the copulatory bursa between the two haplotypes. The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

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Section: Discussionsupporting

confidence: 89%

Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles

Monte

Gentile

Garcia

et al. 2014

Mem. Inst. Oswaldo Cruz

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“…Lee et al. (2014) also reported greater pathogenicity and survival in rats infected with the P isolate. They indicated no differences in male body size, bursal number, bursal shape, or spicule morphology (though sizes not given).…”

Section: Discussionmentioning

confidence: 92%

“…Nonetheless, they reported significantly lower recovery of adult worms from rats for the H isolate compared to the P isolate and smaller female body size in the H isolate. Lee et al (2014) also reported greater pathogenicity and survival in rats infected with the P isolate. They indicated no differences in male body size, bursal number, bursal shape, or spicule morphology (though sizes not given).…”

Section: Angiostrongylus Cantonensis a Malaysiensis And A Mackerrmentioning

confidence: 89%

“…The geographic affiliations of subclades given in Table 3 suggest global distributions could be limited to just some subclades. Finer genetic marker resolution is needed because existing studies suggest variation in life history or pathogenicity among lineages (Lee et al., 2014; Monte et al., 2012). More studies are necessary to determine whether mtDNA subclades (possibly representing a species complex) are associated with life‐history, infectivity, or pathogenicity traits.…”

Section: Discussionmentioning

confidence: 99%

“…(2012) also report greater larval output with infections of CO1 ac9. In Lee et al (2014), morphology and pathogenicity were examined between two CO1 mtDNA isolates, Pingtung (P) and Hualien (H), identified as A. cantonensis from Taiwan. There is difficulty in interpreting their sequence data (see Table S2).…”

Section: Angiostrongylus Cantonensis a Malaysiensis And A Mackerrmentioning

confidence: 99%

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Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Dusitsittipon

Criscione

Morand

et al. 2018

Evolutionary Applications

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Angiostrongylus cantonensis, the rat lungworm, is a zoonotic pathogen that is one of the leading causes of eosinophilic meningitis worldwide. This parasite is regarded as an emerging pathogen with a global range expansion out of southeastern Asia post‐WWII. To date, molecular systematic/phylogeographic studies on A. cantonensis have mainly used two mitochondrial (mtDNA) markers, cytochrome c oxidase 1 (CO1) and cytochrome b (CYTB), where the focus has largely been descriptive in terms of reporting local patterns of haplotype variants. In order to look for more global evolutionary patterns, we herein provide a collective phylogenetic assessment using the six available whole mtDNA genome samples that have been tagged as A. cantonensis, A. malaysiensis, or A. mackerrasae along with all other GenBank CO1 and CYTB partial sequences that carry these species identifiers. The results reveal three important complications that researchers will need to be aware of, or will need to resolve, prior to conducting future molecular evolutionary studies on A. cantonensis. These three problems are (i) incongruence between taxonomic identifications and mtDNA variants (haplotypes or whole mtDNA genome samples), (ii) the presence of a CYTB mtDNA pseudogene, and (iii) the need to verify A. mackerrasae as a species along with other possible cryptic lineages, of which there is suggestive evidence (i.e., A. cantonensis could be a species complex). We provided a discussion of how these complications are hurdles to our understanding of the global epidemiology of angiostrongyliasis. We call for future studies to be more explicit in morphological traits used for identifications (e.g., provide measurements). Moreover, it will be necessary to repeat prior morphological and life‐history studies while simultaneously using sequence data in order to assess possible associations between critical epidemiological data (e.g., biogeography, virulence/pathology, host species use) and specific lineages.

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Identification and genetic characterization of Angiostrongylus cantonensis isolated from the human eye

et al. 2023

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Sequence analysis in partial genes of five isolates of Angiostrongylus cantonensis from Taiwan and biological comparison in infectivity and pathogenicity between two strains

Cited by 21 publications

References 44 publications

Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles

Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Identification and genetic characterization of Angiostrongylus cantonensis isolated from the human eye

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