Sequence analysis and structure prediction of ABHD16A and the roles of the ABHD family members in human disease

Abstract

Abhydrolase domain containing 16A (ABHD16A) is a member of the α/β hydrolase domain-containing (ABHD) protein family and is expressed in a variety of animal cells. Studies have shown that ABHD16A has acylglycerol lipase and phosphatidylserine lipase activities. Its gene location in the main histocompatibility complex (MHC) III gene cluster suggests that this protein may participate in the immunomodulation of the body. The results of studies investigating nearly 20 species of ABHDs reveal that the ABHD proteins are key factors in metabolic regulation and disease occurrence and development. In this paper, we summarize the related progress regarding the function of ABHD16A and other ABHD proteins. A prediction of the active sites and structural domains of ABHD16A and an analysis of the amino acid sites are included. Moreover, we analysed the amino acid sequences of the ABHD16A molecules in different species and provide an overview of the related functions and diseases associated with these proteins. The functions and diseases related to ABHD are systematically summarized and highlighted. Future research directions for studies investigating the functions and mechanisms of these proteins are also suggested. Further studies investigating the function of ABHD proteins may further confirm their positions as important determinants of lipid metabolism and related diseases.

The dynamics of gene expression during and post meiosis sets the sperm agenda

“…While most transcripts showed a decline as we moved from spermatocytes to sperm, two transcripts gradually increased their level till the formation of spermatozoa. These transcripts are interestingly known to play significant roles in postfertilization development (Fang et al, ; Xu et al, ). Among other transcripts, reappearance of six transcripts in sperm remains interesting.…”

“…While most transcripts showed a decline as we moved from spermatocytes to sperm, two transcripts gradually increased their level till the formation of spermatozoa. These transcripts are interestingly known to play significant roles in postfertilization development (Fang et al, 2019;Xu et al, 2018 access to the standard pellet diet and water ad libitum, were used in these investigations. At autopsy, the testes and epididymis were dissected out for isolation and purification of primary spermatocytes, round spermatids (from testis) and sperm (from cauda epididymis).…”

Trafficking of Stretch-Regulated TRPV2 and TRPV4 Channels Inferred Through Interactomics

“…The lipase/acyl-transferase dehydrogenase enzyme (ABHD16A) is a TRPV4 interactor involved in lipid metabolism [49]. Proteins of the ABHD family are involved in lipidic modifications, such as palmitoylation, and in negative regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking.…”

“…Proteins of the ABHD family are involved in lipidic modifications, such as palmitoylation, and in negative regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The depalmitoylase ABHD17A is crucial for synaptic targeting and vesicle sorting of AMPA receptors, through PSD-95 depalmitoylation [49,50]. In line with AMPA, but also to N-methyl-d-aspartate (NMDA) receptors trafficking, the calcium/calmodulin dependent protein kinase II beta (CAMK2B) and the calcium/calmodulin dependent serine protein kinase (CASK), both TRPV4 interactors, are trafficking regulatory proteins [51][52][53].…”

“…Viral infection hijacks AnxA2, which is used for the virus advantage in cell-attachment, replication, and proliferation processes [60]; thus, AnxA2 is a likely candidate protein to play a role in the TRPV4-DDX3X mechanism in viral infectivity [22]. The lipase/acyl-transferase dehydrogenase enzyme (ABHD16A) is a TRPV4 interactor involved in lipid metabolism [49]. Proteins of the ABHD family are involved in lipidic modifications, such as palmitoylation, and in negative regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking.…”