Sequence analysis and product assignment of segment 7 of the rice dwarf virus genome

Viruses in the Phytoreovirus Genus of the Reoviridae Family have the Same Conserved Terminal Sequences

“…The terminal sequences of several segments of RDV and RGDV have been determined by Omura et al (1988Omura et al ( , 1989, Fukumoto et al (1989), Nakashima et al (1990) and Koganezawa et al (1990) using apparently full-length cDNA clones. The direct RNA analyses presented here confirmed the terminal sequences of several of the RDV and RGDV genome segments determined previously.…”

“…The Y-terminal sequences of all 12 segments were identical for the first four nucleotides, 5' AUCA---, except for the fourth residue of segment 9 which was G ( Table 1). The sequences derived were those of a minus strand because they were complementary to the putative conserved 3'-terminal tetranucleotide, ---UGAU 3', which had been found previously in eight other RDV genome segments (Uyedaetal., 1987(Uyedaetal., , 1989(Uyedaetal., , 1990Omuraetal., 1988Omuraetal., , 1989Suzuki et al, 1989Suzuki et al, , 1990aFukumoto et al, 1989;Nakashima et al, 1990;Yamada et al, 1990).…”

Segment 5 of the Rice Dwarf Virus Genome Encodes a Protein Highly Conserved within the Phytoreoviruses

“…The Y-terminal nucleotide sequence of 5' GGCAAA is the same as those of RDV $7 (Nakashima et al, 1990) and$8 (Omura et al, 1989). The three large clones were Y-coterminal which suggests that the sequence is full length.…”

A reovirus in the brown planthopper, Nilaparvata lugens

“…Exact Mr values for the segments of RDV were obtained by comparison to the fully sequenced segments, $3 to SI0 of RDV (Uyeda et al, 1987(Uyeda et al, , 1989(Uyeda et al, , 1990Omura et al, 1988Omura et al, , 1989Fukumoto et al, 1989;Nakashima et al, 1990; large amount in the core. The discrepancy of the mobility of 135K proteins between lanes 1 and 3 is thought to be brought about by the different amounts of protein loaded.…”