Septum formation in Aspergillus nidulans

Harris, Steven D.

doi:10.1016/s1369-5274(01)00276-4

Cited by 102 publications

(91 citation statements)

References 37 publications

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“…Furthermore, in most filamentous fungi, a small pore is retained to enable communication between adjacent hyphal compartments. Septum formation has been studied in several filamentous fungi, including Aspergillus nidulans (Harris 2001;Walther and Wendland 2003). Upon germination of asexual conidiospores in A. nidulans, the first few rounds of parasynchronous nuclear division are not accompanied by septation until cells reach an appropriate size/ volume (Harris et al 1994;Wolkow et al 1996).…”

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confidence: 99%

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Regulation of Septum Formation by the Bud3–Rho4 GTPase Module inAspergillus nidulans

Justa-Schuch

Seiler

et al. 2010

Genetics

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The ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. The bud site selection system of Saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. However, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. Here, we describe the functional characterization and localization of the Aspergillus nidulans homolog of the axial bud site marker Bud3. Our results show that AnBud3 is not required for polarized hyphal growth per se, but is involved in septum formation. In particular, our genetic and biochemical evidence implicates AnBud3 as a guanine nucleotide exchange factor for the GTPase Rho4. Additional results suggest that the AnBud3-Rho4 module acts downstream of the septation initiation network to mediate recruitment of the formin SepA to the site of contractile actin ring assembly. Our observations provide new insight into the signaling pathways that regulate septum formation in filamentous fungi.

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confidence: 99%

“…Genetic analyses have identified several functions required for septum formation in A. nidulans, including the septation initiation network (SIN), the septins, and a formin (Harris 2001). The SIN is a cascade of three protein kinases that is activated by a small GTPase (Krapp and Simanis 2008).…”

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confidence: 99%

Regulation of Septum Formation by the Bud3–Rho4 GTPase Module inAspergillus nidulans

Justa-Schuch

Seiler

et al. 2010

Genetics

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“…Haploid fungal organisms like the unicellular yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe and the filamentous fungus Aspergillus nidulans serve as model systems for dissecting fundamental mechanisms that bring about cytokinesis in eukaryotes (Harris 2001;Krapp et al 2004). Cytokinesis in fungi, or septation, results in the formation of a cross wall known as the septum.…”

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Isolation of Mutations That Bypass the Requirement of the Septation Initiation Network for Septum Formation and Conidiation inAspergillus nidulans

et al. 2006

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The kinase cascade of the septation initiation network (SIN), first revealed in fission yeast, activates the contraction of the actomyosin ring, and plays an essential role in fungal septation. Mob1p, an evolutionarily conserved SIN protein, is associated with the most downstream kinase of this cascade in fission yeast. In this study, the mobA gene encoding a homologous protein was isolated from the filamentous fungus Aspergillus nidulans, whose mycelium is made of multinucleate cells. The MOBA protein was required for septation and conidiation, but was not essential for hyphal extension and colony formation. To identify genes that act antagonistically against the SIN, UV mutagenesis was carried out to isolate suppressor (smo) mutations that restored conidiation when MOBA was not expressed. Microscopic examination indicated that the restored conidiation was concomitant with restored septation in the absence of the MOBA protein. Eight recessive smo mutations in five complementation groups also bypassed the requirement of the SIN kinases SEPH and SIDB for septum formation and conidiation. However, none of these smo mutations affected the localization of MOBA. Among smo mutations, smoA and smoB mutations caused reduced hyphal growth and colony formation. They also rendered hypersensitivity to low doses of the microtubule-depolymerizing agent benomyl for conidiation. Therefore, in A. nidulans, proteins encoded by the smo genes likely have an antagonistic interaction against the SIN pathway to regulate septation and conidiation.

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“…Th ese are that a hypha grows only at its apex (BartnickiGarcia, 2002;Momany, 2002), and that cross walls form only at right angles to the long axis of the hypha (Harris, 2001). Th e consequence of these features is that no amount of cross wall formation (cell division) in fungi will turn one hypha into two hyphae (Fıeld et al, 1999;Howard and Gow, 2001;Momany, 2001;Momany et al, 2001).…”

Section: Kingdom Fungimentioning

confidence: 99%

Principles of Mushroom Developmental Biology

Moore

2005

Int J Med Mushr

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ABSTRACT:Research done over the last century has persistently indicated major diff erences between fungi, animals, and plants. Unfortunately, for most of that time fungi have been considered, quite erroneously, to be closely related to plants; as observations have been constrained to comply with this fundamental error, a proper appreciation of fungal developmental biology has been seriously inhibited. During the fi nal quarter of the 20t century, the phylogenetic status of the true fungi as an independent Kingdom of eukaryotes became clear. In this review, I bring together some of the observations, old and recent, that contribute to our current understanding of the way that fungi construct multicellular structures.

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Septum formation in Aspergillus nidulans

Cited by 102 publications

References 37 publications

Regulation of Septum Formation by the Bud3–Rho4 GTPase Module inAspergillus nidulans

Regulation of Septum Formation by the Bud3–Rho4 GTPase Module inAspergillus nidulans

Isolation of Mutations That Bypass the Requirement of the Septation Initiation Network for Septum Formation and Conidiation inAspergillus nidulans

Principles of Mushroom Developmental Biology

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