Septo-hippocampal neurons in the aged rat: Relation between their electrophysiological and pharmacological properties and behavioral performances

Lamour, Y.; Bassant, M.H.; Jobert, Antoinette; Joly, Mélanie

doi:10.1016/0197-4580(89)90028-6

Cited by 37 publications

(15 citation statements)

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“…The type 1 theta requires a connection to the isocortex via the entorhinal cortex, rather than depending on a particular neurotransmitter system [26]. A few reports have described changes in rat hippocampal theta with aging [13,16], however, the correlation between the two types of related behavior because it is a simple behavior to distinguish from LIA related behavior, such as immobility. The type 1 theta recordings from the walking rats were sampled and analyzed (Fig.…”

Section: Methodsmentioning

confidence: 98%

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Age-Related Changes in Rat Hippocampal Theta Rhythms: A Difference between Type 1 and Type 2 Theta.

Abe

Toyosawa

1999

The Journal of Veterinary Medical Science

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ABSTRACT. The age-related changes in two types of theta rhythms recorded from the hippocampus in young (4 months-old), mature (12-13 months-old) and aged (22-25 months-old) rats were investigated. The type 1 theta rhythm was measured from hippocampal EEG recorded from walking rats and the type 2 theta was measured from the EEG induced by reticular pontin oralis nucleus (PON) stimulation in urethane anesthetized rats. The peak frequency and the peak power were detected from power spectra calculated on each theta sample by fast Fourier transformation (FFT). No age-related alteration was observed on the peak frequency of type 1 theta rhythm. However, on type 2 theta rhythm, the peak frequency was decreased in the aged rats compared with the young and the mature rats. The type 2 theta rhythm is cholinergic, and therefore this result suggests that age-related deterioration can be clearly observed in the cholinergic system including the hippocampus in rats.-KEY WORDS: aging, hippocampus, rat, theta rhythm.J. Vet. Med. Sci. 61 (5): [543][544][545][546][547][548] 1999 theta rhythms and aging remains unclear.In this study, we attempted to clarify the age-related changes in the two types of hippocampal theta rhythms associated with different pharmacological systems. MATERIALS AND METHODSMale Wistar rats supplied by SLC Co., Ltd. were used in this study. For the measurement of the type 1 theta, three aged rats (22-25 months-old), four mature rats (12-13 months-old) and four young rats (3-4 months-old) were used. For the type 2 theta, four aged rats, four mature rats and three young rats were used. Aged and mature rats were purchased as retired rats (6-7 months-old) and housed in our animal facility for 6 months and 18 months respectively. The animal facility was maintained on a 12 hr light/dark cycle, at 21 ± 1°C and 50-60% humidity.The rats used for the type 1 theta experiments were deeply anesthetized by pentobarbital sodium (50 mg/kg, i.p.) and then placed into a stereotaxic frame. A pair of staggered electrodes (200 µm stainless steel wires insulated except at cut ends) were chronically implanted in the bilateral hippocampal formation. The deep electrode was directed at the molecular layer of the dentate gyrus (DG), and the surface electrode at the CA1 pyramidal cell layer. Two stainless steel screw electrodes for reference and ground were fixed in the skull above the cerebellum. After 1 week of recovery from surgery, the type 1 theta recordings were carried out. The rats were placed in an open field box (100 × 70 × 33 cm) without a roof, which was painted gray. The hippocampal EEG and the behavioral data in the freely moving rats were recorded and stored simultaneously on a video tape using a video converter system (NEC Sanei) which received the signals from a multichannel amplifier and a CCD camera. Walking was selected as a type 1 theta

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Section: Methodsmentioning

confidence: 98%

“…Therefore age-related impairment of these functions might be due at least in part to alterations in the hippocampus. The morphological and neurochemical age-related changes in rat hippocampus are well documented [3,4,8,9,14,15,22,23,30], but fewer studies have examined the age-related changes in electrophysiological properties [13,15,16,19,21].…”

Section: Methodsmentioning

confidence: 99%

Age-Related Changes in Rat Hippocampal Theta Rhythms: A Difference between Type 1 and Type 2 Theta.

Abe

Toyosawa

1999

The Journal of Veterinary Medical Science

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“…These deficits were reminiscent of those produced by the nonselective muscarinic antagonist scopolamine or by ligands that impair cognition by dysregulating cholinergic neurotransmission at the level of the septohippocampal pathway. [42][43][44][45]49,50 Dysregulated acetylcholine release in response to environmental stimuli and deficits in the passive avoidance paradigm are not likely to be because of nonspecific phenotypic alterations such as hyperlocomotion in the KO mice. In fact, M4-KO mice that display increased basal locomotor activity 9 do not show deficits in the passive avoidance, whereas M2-KO mice, that perform poorly in the passive avoidance test, display normal locomotor activity (unpublished observations).…”

Section: Discussionmentioning

confidence: 99%

“…Although the passive avoidance paradigm is a nonspecific aversive memory test that involves a number of brain structures, it exhibits well-documented validity in depicting memory impairments as a result of aging, or pharmacological and genetic manipulations. [42][43][44][45][46] Acquisition and/or retention in the passive avoidance test are dependent on the integrity of the hippocampal cholinergic pathway. 35,[42][43][44]47,48 M2-and M2/M4-KO mice, but not M4-KO mice, showed impaired memory retention in the passive avoidance paradigm.…”

Section: Discussionmentioning

confidence: 99%

“…[42][43][44][45][46] Acquisition and/or retention in the passive avoidance test are dependent on the integrity of the hippocampal cholinergic pathway. 35,[42][43][44]47,48 M2-and M2/M4-KO mice, but not M4-KO mice, showed impaired memory retention in the passive avoidance paradigm. These deficits were reminiscent of those produced by the nonselective muscarinic antagonist scopolamine or by ligands that impair cognition by dysregulating cholinergic neurotransmission at the level of the septohippocampal pathway.…”

Section: Discussionmentioning

confidence: 99%

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Dysregulated hippocampal acetylcholine neurotransmission and impaired cognition in M2, M4 and M2/M4 muscarinic receptor knockout mice

et al. 2003

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Among the five different muscarinic receptors that have been cloned and characterized, M2 and M4 receptors are localized both post-and presynaptically and are believed to have a pronounced autoreceptor role. The functional importance of these receptors in the regulation of acetylcholine release in the hippocampus and in cognitive processes was investigated by using M2 and M4 receptor single knockout (KO) as well as M2/M4 receptor double KO mice. We found profound alterations in acetylcholine homeostasis in the hippocampus of both M2-and M4-KO mice as well as of the combined M2/M4-KOs, as assessed by in vivo microdialysis. Basal acetylcholine efflux in the hippocampus was significantly increased in M4-KO and was elevated further in M2/M4-KOs. The increase in hippocampal acetylcholine induced by local administration of scopolamine was markedly reduced in M2-KO and completely abolished in M2/M4-KOs. In M2-KO and much more in M2/M4-KOs, the increase in hippocampal acetylcholine triggered by exposure to a novel environment was more pronounced both in amplitude and duration, with a similar trend observed for M4-KOs. Dysregulation of cholinergic function in the hippocampus, as it could result from perturbed autoreceptor function, may be associated with cognitive deficits. Importantly, M2-and M2/M4-KO, but not M4-KO, animals showed an impaired performance in the passive avoidance test. Together these results suggest a crucial role for muscarinic M2 and M4 receptors in the tonic and phasic regulation of acetylcholine efflux in the hippocampus as well as in cognitive processes.

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The theta‐related firing activity of parvalbumin‐positive neurons in the medial septum‐diagonal band of broca complex and their response to 5‐HT_1A Receptor stimulation in a rat model of Parkinson's disease

Han

Zhang

et al. 2013

Hippocampus

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The parvalbumin (PV)-positive neurons in the medial septum-diagonal band of Broca complex (MS-DB) play an important role in the generation of hippocampal theta rhythm involved in cognitive functions. These neurons in this region express a high density of 5-HT1A receptors which regulate the neuronal activity and consequently affect the theta rhythm. In this study, we examined changes in the theta-related firing activity of PV-positive neurons in the MS-DB, their response to 5-HT1A receptor stimulation and the corresponding hippocampal theta rhythm, and the density of PV-positive neurons and their co-localization with 5-HT1A receptors in rats with 6-hydroxydopamine lesions of the substantia nigra pars compacta (SNc). The lesion of the SNc decreased the rhythmically bursting activity of PV-positive neurons and the peak frequency of hippocampal theta rhythm. Systemic administration of 5-HT1A receptor agonist 8-OH-DPAT (0.5-128 µg/kg, i.v.) inhibited the firing rate of PV-positive neurons and disrupted rhythmically bursting activity of the neurons and the theta rhythm in sham-operated and the lesioned rats, respectively. The cumulative doses producing inhibition and disruption in the lesioned rats were higher than that of sham-operated rats. Furthermore, local application of 8-OH-DPAT (0.005 μg) in the MS-DB also inhibited the firing rate of PV-positive neurons and disrupted their rhythmically bursting activity in sham-operated rats, while having no effect on PV-positive neurons in the lesioned rats. The lesion of the SNc decreased the density of PV-positive neurons in the MS-DB, and percentage of PV-positive neurons expressing 5-HT1A receptors. These results indicate that the lesion of the SNc leads to suppression of PV-positive neurons in the MS-DB and hippocampal theta rhythm. Furthermore, the lesion decreases the response of these neurons to 5-HT1A receptor stimulation, which attributes to dysfunction and/or down-regulation of 5-HT1A receptor expression on these neurons. These changes may be involved in cognitive impairments of Parkinson's disease.

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Septo-hippocampal neurons in the aged rat: Relation between their electrophysiological and pharmacological properties and behavioral performances

Cited by 37 publications

References 28 publications

Age-Related Changes in Rat Hippocampal Theta Rhythms: A Difference between Type 1 and Type 2 Theta.

Age-Related Changes in Rat Hippocampal Theta Rhythms: A Difference between Type 1 and Type 2 Theta.

Dysregulated hippocampal acetylcholine neurotransmission and impaired cognition in M2, M4 and M2/M4 muscarinic receptor knockout mice

The theta‐related firing activity of parvalbumin‐positive neurons in the medial septum‐diagonal band of broca complex and their response to 5‐HT_1A Receptor stimulation in a rat model of Parkinson's disease

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Septo-hippocampal neurons in the aged rat: Relation between their electrophysiological and pharmacological properties and behavioral performances

Cited by 37 publications

References 28 publications

Age-Related Changes in Rat Hippocampal Theta Rhythms: A Difference between Type 1 and Type 2 Theta.

Age-Related Changes in Rat Hippocampal Theta Rhythms: A Difference between Type 1 and Type 2 Theta.

Dysregulated hippocampal acetylcholine neurotransmission and impaired cognition in M2, M4 and M2/M4 muscarinic receptor knockout mice

The theta‐related firing activity of parvalbumin‐positive neurons in the medial septum‐diagonal band of broca complex and their response to 5‐HT1A Receptor stimulation in a rat model of Parkinson's disease

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The theta‐related firing activity of parvalbumin‐positive neurons in the medial septum‐diagonal band of broca complex and their response to 5‐HT_1A Receptor stimulation in a rat model of Parkinson's disease