Septin Organization and Functions in Budding Yeast

Glomb, Oliver; Gronemeyer, Thomas

doi:10.3389/fcell.2016.00123

Cited by 28 publications

(44 citation statements)

References 67 publications

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“…2 E-G). Therefore, PM diffusion barriers may have selectivity or exist only at specific stages of the cell cycle that determine particular complex septin structures (51). Additionally, as previously noted (52)(53)(54), other functions of septins may be responsible for the budor bud neck-enriched localization of PM-associating proteins.…”

Section: Discussionmentioning

confidence: 91%

Distinct segregation patterns of yeast cell-peripheral proteins uncovered by a method for protein segregatome analysis

Sugiyama

Tanaka

2019

Proc. Natl. Acad. Sci. U.S.A.

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Protein segregation contributes to various cellular processes such as polarization, differentiation, and aging. However, the difficulty in global determination of protein segregation hampers our understanding of its mechanisms and physiological roles. Here, by developing a quantitative proteomics technique, we globally monitored segregation of preexisting and newly synthesized proteins during cell division of budding yeast, and identified crucial domains that determine the segregation of cell-peripheral proteins. Remarkably, the proteomic and subsequent microscopic analyses demonstrated that the flow through the bud neck of the proteins that harbor both endoplasmic reticulum (ER) membrane-spanning and plasma membrane (PM)-binding domains is not restricted by the previously suggested ER membrane or PM diffusion barriers but by septin-mediated partitioning of the PM-associated ER (pmaER). Furthermore, the proteomic analysis revealed that although the PM-spanning t-SNARE Sso2 was retained in mother cells, its paralog Sso1 unexpectedly showed symmetric localization. We found that the transport of Sso1 to buds was required for enhancement of polarized cell growth and resistance to cell-wall stress. Taken together, these data resolve long-standing questions about septin-mediated compartmentalization of the cell periphery, and provide new mechanistic insights into the segregation of cell-periphery proteins and their cellular functions.

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Section: Discussionmentioning

confidence: 91%

Distinct segregation patterns of yeast cell-peripheral proteins uncovered by a method for protein segregatome analysis

Sugiyama

Tanaka

2019

Proc. Natl. Acad. Sci. U.S.A.

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“…A set of proteins that also localizes very early to the nascent bud site in S. cerevisiae comprises the mitotic septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p and Shs1p/Sep7p) [35,[87][88][89][90][91]. The septins are GTP-binding proteins that form hetero-octameric rod-shaped complexes which then assemble to form long (32-100 nm) membrane-associated filaments that are 7-9 nm in diameter [35,[88][89][90][91][92]. These septin filaments assemble into a small ring that surrounds the nascent bud site [35,87,90,91,93,94].…”

Section: Role Of Septins In Defining the Nascent Bud Site And Bud Necmentioning

confidence: 99%

“…The septins are GTP-binding proteins that form hetero-octameric rod-shaped complexes which then assemble to form long (32-100 nm) membrane-associated filaments that are 7-9 nm in diameter [35,[88][89][90][91][92]. These septin filaments assemble into a small ring that surrounds the nascent bud site [35,87,90,91,93,94]. They remain at the bud neck throughout the cell cycle, but undergo rearrangements/remodelling to form different structures at different stages of the cell cycle [35,87,90,91,94].…”

Section: Role Of Septins In Defining the Nascent Bud Site And Bud Necmentioning

confidence: 99%

Yeast as a Model to Understand Actin-Mediated Cellular Functions in Mammals—Illustrated with Four Actin Cytoskeleton Proteins

Akram

Ahmed

Mack

et al. 2020

Cells

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The budding yeast Saccharomyces cerevisiae has an actin cytoskeleton that comprises a set of protein components analogous to those found in the actin cytoskeletons of higher eukaryotes. Furthermore, the actin cytoskeletons of S. cerevisiae and of higher eukaryotes have some similar physiological roles. The genetic tractability of budding yeast and the availability of a stable haploid cell type facilitates the application of molecular genetic approaches to assign functions to the various actin cytoskeleton components. This has provided information that is in general complementary to that provided by studies of the equivalent proteins of higher eukaryotes and hence has enabled a more complete view of the role of these proteins. Several human functional homologues of yeast actin effectors are implicated in diseases. A better understanding of the molecular mechanisms underpinning the functions of these proteins is critical to develop improved therapeutic strategies. In this article we chose as examples four evolutionarily conserved proteins that associate with the actin cytoskeleton: (1) yeast Hof1p/mammalian PSTPIP1, (2) yeast Rvs167p/mammalian BIN1, (3) yeast eEF1A/eEF1A1 and eEF1A2 and (4) yeast Yih1p/mammalian IMPACT. We compare the knowledge on the functions of these actin cytoskeleton-associated proteins that has arisen from studies of their homologues in yeast with information that has been obtained from in vivo studies using live animals or in vitro studies using cultured animal cell lines.

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“…Septins are conserved GTP-binding proteins that create the septum in eukaryotes, often serving as scaffolds for other proteins that direct cell cycle progression (51–53). In S. cerevisiae , the septin proteins assemble into filaments at the mother bud neck, creating the hourglass-shaped septum, and are required for normal cytokinesis (53). The C. neoformans septins have been shown to function similarly.…”

Section: Discussionmentioning

confidence: 99%

A fungal arrestin protein contributes to cell cycle progression and pathogenesis

Telzrow

Nichols²,

Castro-Lopez

et al. 2019

Preprint

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word count: 231 19 Text word count: 5,955 20 2 ABSTRACT 21 Arrestins, a structurally specialized and functionally diverse group of proteins, are 22 central regulators of adaptive cellular responses in eukaryotes. Previous studies on 23 fungal arrestins have demonstrated their capacity to modulate diverse cellular 24 processes through their adaptor functions, facilitating the localization and function of 25 other proteins. However, the mechanisms by which arrestin-regulated processes are 26involved in fungal virulence remain unexplored. We have identified a small family of four 27 arrestins -Ali1, Ali2, Ali3, and Ali4 -in the human fungal pathogen Cryptococcus 28 neoformans. Using complementary microscopy, proteomic, and reverse genetic 29 techniques, we have defined a role for Ali1 as a novel contributor to cytokinesis, a 30 fundamental cell cycle-associated process. We observed that Ali1 strongly interacts with 31 proteins involved in lipid synthesis, and that ali1 mutant phenotypes are rescued by 32 supplementation with lipid precursors that are used to build cellular membranes. From 33 these data, we hypothesize that Ali1 contributes to cytokinesis by serving as an adaptor 34 protein, facilitating the localization of enzymes that modify the plasma membrane during 35 cell division, specifically the fatty acid synthases, Fas1 and Fas2. Finally, we assessed 36 the contributions of the C. neoformans arrestin family to virulence, to better understand 37 the mechanisms by which arrestin-regulated adaptive cellular responses influence 38 fungal infection. We observed that the C. neoformans arrestin family contributes to 39 virulence, and that the individual arrestin proteins likely fulfill distinct functions that are 40 important for disease progression. 41 3 IMPORTANCE 42 To survive in unpredictable conditions, all organisms must adapt to stressors by 43 regulating adaptive cellular responses. Arrestin proteins are conserved regulators of 44 adaptive cellular responses in eukaryotes. Studies that have been limited to mammals 45 and model fungi have demonstrated that disruption of arrestin-regulated pathways is 46 detrimental for viability. The human fungal pathogen Cryptococcus neoformans causes 47 56

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Septin Organization and Functions in Budding Yeast

Cited by 28 publications

References 67 publications

Distinct segregation patterns of yeast cell-peripheral proteins uncovered by a method for protein segregatome analysis

Distinct segregation patterns of yeast cell-peripheral proteins uncovered by a method for protein segregatome analysis

Yeast as a Model to Understand Actin-Mediated Cellular Functions in Mammals—Illustrated with Four Actin Cytoskeleton Proteins

A fungal arrestin protein contributes to cell cycle progression and pathogenesis

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