Septin mutations and phenotypes in S. cerevisiae

Abstract

Septins are highly conserved guanosine triphosphate (GTP)-binding proteins that are a component of the cytoskeletal systems of virtually all eukaryotes (except higher plants). Septins play important roles in a multitude of cellular processes, including cytokinesis, establishment of cell polarity, and cellular partitioning. The ease of genetic screens and a fully sequenced genome have made Saccharomyces cerevisiae one of the most extensively studied and well-annotated model organisms in eukaryotic biology. Here, we present a synopsis of the known point mutations in the seven S. cerevisiae septin genes: CDC3, CDC10, CDC11, CDC12, SHS1, SPR3, and SPR28. We map these mutations onto septin protein structures, highlighting important conserved motifs, and relating the functional consequences of mutations in each domain.

Genes and lipids that impact uptake and assimilation of exogenous coenzyme Q in Saccharomyces cerevisiae

“…Interaction partners previously identified suggested a role for SEPT9 in vesicle transport to and from the plasma membrane [93]. The other three core septins (Cdc3, Cdc11 and Cdc12) are encoded by essential genes and therefore the null mutants cannot be interrogated [94]. Whether the involvement of Cdc10 (or potentially other septins) in CoQ6 uptake/transport is related to the role of septins in endocytosis or a different septin function, will require further experimentation.…”

The Scaffold Proteins Paxillin B and α-Actinin Regulate Septation in Aspergillus nidulans via Control of Actin Ring Contraction

“…Second, relevant scaffold proteins, which provide functions such as binding and supporting protein interactions, are transported to the division site to assist with the assembly of the actin ring (GE AND BALASUBRAMANIAN 2008;LI et al 2016). Third, a contractile actin ring composed of actin, septin and formin is formed (COURTEMANCHE 2018;MELA AND MOMANY 2019). This step is regulated by a conserved signaling kinase cascade, such as the septation-initiation network (SIN) in Saccharomyces pombe and the mitotic-exit network (MEN) in the budding yeast Saccharomyces cerevisiae (MULVIHILL et al 2001;CORBETT et al 2006).…”

Editorial overview: The molecular and cellular biology of septins

“…Perez and Thorner present their latest findings, describing how septin associated proteins traffic between the yeast bud neck and nucleus . As a resource to the community, Mela and Momany provide a comprehensive overview of known yeast septin mutations and phenotypes, and discover that a majority o f them reside in the G1 motif of the conserved G-domain essential for septin-septin and septin-nucleotide interactions (Mela and Momany, 2018).…”