Septin 7 is required for orderly meiosis in mouse oocytes

Li, Sen; Ou, Xiang Hong; Liang, Wei; Wang, Zhen Bo; Zhang, Qing; Ouyang, Ying‐Chun; Hou, Yi; Schatten, Heide; Sun, Qing‐Yuan

doi:10.4161/cc.21553

Cited by 30 publications

(30 citation statements)

References 43 publications

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“…After GVBD, microtubules assemble to chromosomes during the prometaphase I (pro MI) stage, and then chromosomes migrate to the central plate of the bipolar spindle and remain in a well-aligned state during the MI stage. 12,13 Subsequently, anaphase I (AI) ensues, followed by extrusion of the first polar body and the spindle migrates to the cortex. Then, the oocyte emits the first polar body, followed by the formation of the metaphase II (MII) spindle located beneath the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Zhuang

Shi

et al. 2014

Cell Cycle

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Gametogenesis is a complex biological process of producing cells for sexual reproduction. Xlr super family members containing a conserved COR1 domain play essential roles in gametogenesis. In the present study, we identified that Slx2, a novel member of Xlr super family, is specifically expressed in the meiotic oocytes, which is demonstrated by western blotting and immunohistochemistry studies. In the first meiotic prophase, SLX2 is unevenly distributed in the nuclei of oocytes, during which phase SLX2 is partly co-localized with SYCP3 in synaptonemal complex and γH2AX in the nucleus of oocytes. Interestingly, the localization of SLX2 was found to be switched into the cytoplasm of oocytes after prometaphase I during oocyte maturation. Furthermore, yeast two-hybrid and coimmunoprecipitation studies demonstrated that SLX2 interacts with BLOS2, which is a novel centrosome-associated protein, and co-localized with γ-Tubulin, which is a protein marker of chromosome segregation in meiosis. These results indicated that SLX2 might get involved in chromosomes segregation during meiosis by interaction with BLOS2. In conclusion, SLX2 might be a novel gametogenesis-related protein that could play multiple roles in regulation of meiotic processes including synaptonemal complex assembly and chromosome segregation.

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Section: Introductionmentioning

confidence: 99%

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Zhuang

Shi

et al. 2014

Cell Cycle

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“…We suggest that the first early peak of embryonic death is caused by oocyte polarization defects. The fact that the formation of the meiotic spindle and the separation of polar bodies were impaired with a decrease or overexpression of SEPT7 protein (Pnut homolog in mammals) in mouse oocytes which finally resulted in chromosomal anomalies and early death can be indirect evidence in favor of our hypothesis [6]. …”

Section: Discussionmentioning

confidence: 99%

“…However, these mutations did not affect the proliferation of germline cells in spermatogenesis [5]. Recent studies indicate that SEPT7 protein (Pnut homolog in mammals) is required for meiosis in mouse oocytes [6]. In the present work we studied peanut gene functions in Drosophila oogenesis.…”

Section: Introductionmentioning

confidence: 98%

Analysis of peanut gene RNAi in drosophila oogenesis

et al. 2015

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The peanut gene functions in Drosophila melanogaster oogenesis were studied. It was demonstrated that the suppression of peanut expression by RNA interference in follicle cells led to oocyte polarization defects, anomalous cytokinesis in the chorion cells, and aberrant chromatin condensation in follicle cells. No oogenesis abnormalities were observed in females with decreased peanut gene expression in ovarian germline cells. However, embryos produced by such females had a decreased survival rate caused by two peaks of embryonic death.

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“…Septin 7 belongs to the evolutionarily conserved group of GTP-binding and filament-forming proteins originally discovered in yeast [43], [44] and recently described in vertebrates as important part of the cell division cycle [45]. Not only do septins interact with the actin cytoskeleton [46] and regulate microtubule stability within the cell [47], they also seem to coordinate changes in membrane organization of cells [42].…”

Section: Discussionmentioning

confidence: 99%

Unraveling the Equine Lymphocyte Proteome: Differential Septin 7 Expression Associates with Immune Cells in Equine Recurrent Uveitis

et al. 2014

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Equine recurrent uveitis is a spontaneous, lymphocyte-driven autoimmune disease. It affects horses worldwide and presents with painful remitting-relapsing inflammatory attacks of inner eye structures eventually leading to blindness. Since lymphocytes are the key players in equine recurrent uveitis, we were interested in potential changes of their protein repertoire which may be involved in disease pathogenesis. To create a reference for differential proteome analysis, we first unraveled the equine lymphocyte proteome by two-dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis and subsequently identified 352 protein spots. Next, we compared lymphocytes from ERU cases and healthy horses with a two-dimensional fluorescence difference in gel electrophoresis approach. With this technique, we identified seven differentially expressed proteins between conditions. One of the significantly lower expressed candidates, septin 7, plays a role in regulation of cell shape, motility and migration. Further analyses revealed T cells as the main cell type with decreased septin 7 abundance in equine recurrent uveitis. These findings point to a possible pathogenetic role of septin 7 in this sight-threatening disease.

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Septin 7 is required for orderly meiosis in mouse oocytes

Cited by 30 publications

References 43 publications

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Analysis of peanut gene RNAi in drosophila oogenesis

Unraveling the Equine Lymphocyte Proteome: Differential Septin 7 Expression Associates with Immune Cells in Equine Recurrent Uveitis

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