Septic arthritis due to a Sneathia species most closely related to Sneathia sanguinegens

Bachy, Bruno; Bémer, Pascale; Tortellier, L.; Giraudeau, C.; Reynaud, Alain; Corvec, Stéphane

doi:10.1099/jmm.0.027458-0

Cited by 14 publications

(8 citation statements)

References 11 publications

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“…S. sanguinegens has been found as part of the micro-flora of intra-amniotic infection in humans in which it was as prevalent as the most frequent invaders of the amniotic cavity ( Mycoplasma spp.) (27), and using 16S rRNA gene sequencing, S. sanguinegens has also been identified in cases of septic arthritis (29) and late onset bronchiolitis obliterans syndrome (30). Thus, its pathogenic significance is becoming more appreciated.…”

Section: Discussionmentioning

confidence: 99%

A Metataxonomic Approach Could Be Considered for Cattle Clinical Mastitis Diagnostics

et al. 2017

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Mastitis is one of the most costly diseases affecting the dairy industry, and identification of the causative microorganism(s) is essential. Here, we report the use of next-generation sequencing of bacterial 16S rRNA genes for clinical mastitis diagnosis. We used 65 paired milk samples, collected from the mastitic and a contralateral healthy quarter of mastitic dairy cattle to evaluate the technique as a potential alternative to bacterial culture or targeted PCR. One large commercial dairy farm was used, with one trained veterinarian collecting the milk samples. The 16S rRNA genes were individually amplified and sequenced using the MiSeq platform. The MiSeq Reporter was used in order to analyze the obtained sequences. Cattle were categorized according to whether or not 1 of the 10 most abundant bacterial genera in the mastitic quarter exhibited an increase in relative abundance between the healthy and mastitic quarters equal to, or exceeding, twofold. We suggest that this increase in relative abundance is indicative of the genus being a causative mastitis pathogen. Well-known mastitis-causing pathogens such as Streptococcus uberis and Staphylococcus spp. were identified in most cattle. We were able to diagnose 53 out of the 65 studied cases and identify potential new mastitis pathogens such as Sneathia sanguinegens and Listeria innocua, which are difficult to identify by bacterial culture because of their fastidious nature.

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Section: Discussionmentioning

confidence: 99%

A Metataxonomic Approach Could Be Considered for Cattle Clinical Mastitis Diagnostics

et al. 2017

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“…These findings suggest that Sneathia has the pathogenic capacity to invade the uterine cavity and the amniotic sac and thereby cause pregnancy complications. Sneathia has also been implicated in both infant and maternal post-partum bacteremia [11-13]. More recently, a case of septic arthritis in a healthy, non-pregnant woman was reported, demonstrating that this opportunistic pathogen has the potential to cause infections outside of the reproductive tract as well [11-13].…”

Section: Introductionmentioning

confidence: 99%

Genomic sequence analysis and characterization of Sneathia amnii sp. nov

Harwich

Serrano²,

Fettweis³

et al. 2012

BMC Genomics

115

100

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BackgroundBacteria of the genus Sneathia are emerging as potential pathogens of the female reproductive tract. Species of Sneathia, which were formerly grouped with Leptotrichia, can be part of the normal microbiota of the genitourinary tracts of men and women, but they are also associated with a variety of clinical conditions including bacterial vaginosis, preeclampsia, preterm labor, spontaneous abortion, post-partum bacteremia and other invasive infections. Sneathia species also exhibit a significant correlation with sexually transmitted diseases and cervical cancer. Because Sneathia species are fastidious and rarely cultured successfully in vitro; and the genomes of members of the genus had until now not been characterized, very little is known about the physiology or the virulence of these organisms.ResultsHere, we describe a novel species, Sneathia amnii sp. nov, which closely resembles bacteria previously designated "Leptotrichia amnionii". As part of the Vaginal Human Microbiome Project at VCU, a vaginal isolate of S. amnii sp. nov. was identified, successfully cultured and bacteriologically cloned. The biochemical characteristics and virulence properties of the organism were examined in vitro, and the genome of the organism was sequenced, annotated and analyzed. The analysis revealed a reduced circular genome of ~1.34 Mbp, containing ~1,282 protein-coding genes. Metabolic reconstruction of the bacterium reflected its biochemical phenotype, and several genes potentially associated with pathogenicity were identified.ConclusionsBacteria with complex growth requirements frequently remain poorly characterized and, as a consequence, their roles in health and disease are unclear. Elucidation of the physiology and identification of genes putatively involved in the metabolism and virulence of S. amnii may lead to a better understanding of the role of this potential pathogen in bacterial vaginosis, preterm birth, and other issues associated with vaginal and reproductive health.

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“…S. sanguinegens has been reported to be susceptible to clindamycin, amoxicillin, amoxicillin-clavulanic acid, penicillins (such as some cephalosporins of 3rd generation or some carbapenems), and resistant to vancomycin [ 9 , 24 ]. M. hominis is an intracellular pathogen with no cell wall, susceptible to tetracyclines, some quinolones (i.e.…”

Section: Discussionmentioning

confidence: 99%

First report of Sneathia sanguinegens together with Mycoplasma hominis in postpartum prosthetic valve infective endocarditis: a case report

et al. 2017

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BackgroundThe presence of more than one bacterial agent is relatively rare in infective endocarditis, although more common in prosthetic cases. Molecular diagnosis from a removed heart tissue is considered a quick and effective way to diagnose fastidious or intracellular agents.Case presentationHere we describe the case of postpartum polymicrobial prosthetic valve endocarditis in a young woman. Sneathia sanguinegens and Mycoplasma hominis were simultaneously detected from the heart valve sample using broad range 16S rRNA polymerase chain reaction (PCR) followed by sequencing while culture remained negative. Results were confirmed by independent PCR combined with denaturing gradient gel electrophoresis. Before the final agent identification, the highly non-compliant patient left from the hospital against medical advice on empirical intravenous treatment with aminopenicillins, clavulanate and gentamicin switched to oral amoxycillin and clavulanate. Four months after surgery, no signs of inflammation were present despite new regurgitation and valve leaflet flail was detected. However, after another 5 months the patient died from sepsis and recurrent infective endocarditis of unclarified etiology.Conclusions Mycoplasma hominis is a rare causative agent of infective endocarditis. To the best of our knowledge, presented case is the first report of Sneathia sanguinegens detected in this condition. Molecular techniques were shown to be useful even in polymicrobial infective endocarditis samples.

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Septic arthritis due to a Sneathia species most closely related to Sneathia sanguinegens

Cited by 14 publications

References 11 publications

A Metataxonomic Approach Could Be Considered for Cattle Clinical Mastitis Diagnostics

A Metataxonomic Approach Could Be Considered for Cattle Clinical Mastitis Diagnostics

Genomic sequence analysis and characterization of Sneathia amnii sp. nov

First report of Sneathia sanguinegens together with Mycoplasma hominis in postpartum prosthetic valve infective endocarditis: a case report

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