Sepsis and septic shock

Mayer, Jiřı́; Vorlíček, Jiří; Tomíška, Miroslav

doi:10.1007/bf00365849

Cited by 9 publications

(1 citation statement)

References 126 publications

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“…The role of cytokines such as tumour necrosis factor (TNF)-α, interleukin (IL)-1 and IL-6 has been proven in sepsis. These lead to a diffuse vasculitis and secondary anoxia in the organs and result in multiple organ dysfunction syndrome [15,18].…”

Section: Discussionmentioning

confidence: 99%

Detection of DNA, mRNA and early antigen of the human cytomegalovirus using the immunomax technique in autopsy material of children with intrauterine infection

Kasprzak¹,

Zabel

Wysocki

et al. 2000

Virchows Archiv

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The present study focuses on the immunomax technique in association with the avidin-biotin-peroxidase complex (ABC) technique and a non-isotopic variation of in situ hybridisation (ISH) for optimal microscopical detection of human cytomegalovirus (HCMV). The studies were performed on an archival paraffin material originating from five children deceased due to intrauterine infection. The results of immunocytochemical and hybridocytochemical studies, with or without amplification using biotinylated tyramine, were compared with the routine histopathological results and results obtained using the polymerase chain reaction (PCR). Early antigen (EA)-HCMV was demonstrated in approximately twice as many cells as detected in the routine staining and also in cells that seemed morphologically intact. The hybridocytochemical studies confirmed the presence of HCMV DNA in cells that were positive in the immunocytochemical tests and, in addition (using the ISH-immunomax technique), in cell nuclei of intact myocardial myocytes. In general, fewer cells manifested the presence of HMCV mRNA than the presence of HCMV DNA. The immunomax technique was found to be more sensitive than the techniques of classical immunocytochemistry or of ISH. The former technique permitted the documentation of a higher number of HCMV replication sites than could be detected using the latter techniques. However, the clinical course of HCMV infection or the cause of death of the children was not directly related to the intensity of HCMV expression in tissues.

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Section: Discussionmentioning

confidence: 99%

Detection of DNA, mRNA and early antigen of the human cytomegalovirus using the immunomax technique in autopsy material of children with intrauterine infection

Kasprzak¹,

Zabel

Wysocki

et al. 2000

Virchows Archiv

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Relationship between contents of adrenomedullin and distributions of neutral endopeptidase in blood and tissues of rats in septic shock

Jiang

Cai

Pan

et al. 2004

Regulatory Peptides

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Traumatic and septic shock alias post-trauma critical illness

Hardaway

1998

Journal of British Surgery

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and conclusion The concept of a shock toxin in trauma and sepsis as promulgated during World War I is correct. This toxin is a thrombogenic aminophospholipid which occurs only on the inner layer of all cell membranes and is liberated by cell destruction. It causes disseminated intravascular coagulation which may obstruct the microcirculation of any or all organs, producing multiple organ failure by microclots. These microclots may be lysed by plasminogen activator and circulation to the organs restored.

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Sepsis and septic shock

Cited by 9 publications

References 126 publications

Detection of DNA, mRNA and early antigen of the human cytomegalovirus using the immunomax technique in autopsy material of children with intrauterine infection

Detection of DNA, mRNA and early antigen of the human cytomegalovirus using the immunomax technique in autopsy material of children with intrauterine infection

Relationship between contents of adrenomedullin and distributions of neutral endopeptidase in blood and tissues of rats in septic shock

Traumatic and septic shock alias post-trauma critical illness

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