Separations of spherical and disc-shaped polystyrene particles and blood components (red blood cells and platelets) using pinched flow fractionation device with a tilted sidewall and vertical focusing channels (t-PFF-v)

Nho, Hyun Woo; Yang, Nuri; Song, Jaewoo; Park, Joon‐Shik; Yoon, Tae Hyun

doi:10.1016/j.snb.2017.04.081

Cited by 14 publications

(12 citation statements)

References 37 publications

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“…Copyright 2011 Springer Nature. (f) Separation of red blood cells and platelets in a PFF device with a tilted sidewall and vertical focusing channels (t-PFF-v) 130 . Reproduced with permission from Nho et al , Sens.…”

Section: Sorting By Pinched Flow Fractionationmentioning

confidence: 99%

“…Recently, a group from Korea reported separation of disk-shaped and spherical particles in their more complex version of PFF device [Fig. 10(f)] with tilted sidewalls and vertical focusing channels (termed t-PFF-v) 130 . Separation of platelets and red blood cells (RBCs) was achieved, and the separation resolution was better in the t-PFF-v device than previous classic PFF microchannels.…”

Section: Sorting By Pinched Flow Fractionationmentioning

confidence: 99%

“…PFF has been widely employed for separation of various particulate samples. While most of the works in the literature are focused on separation of rigid spherical particles, size-based sorting of bubbles, 140 droplets, 126 and biocolloids 130 using PFF has been reported. In recent years, separation of cells of interest has attracted increasing attention owing to its unmet need in biomedical applications 13,14 .…”

Section: Sorting By Pinched Flow Fractionationmentioning

confidence: 99%

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Label-free microfluidic sorting of microparticles

et al. 2019

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Massive growth of the microfluidics field has triggered numerous advances in focusing, separating, ordering, concentrating, and mixing of microparticles. Microfluidic systems capable of performing these functions are rapidly finding applications in industrial, environmental, and biomedical fields. Passive and label-free methods are one of the major categories of such systems that have received enormous attention owing to device operational simplicity and low costs. With new platforms continuously being proposed, our aim here is to provide an updated overview of the state of the art for passive label-free microparticle separation, with emphasis on performance and operational conditions. In addition to the now common separation approaches using Newtonian flows, such as deterministic lateral displacement, pinched flow fractionation, cross-flow filtration, hydrodynamic filtration, and inertial microfluidics, we also discuss separation approaches using non-Newtonian, viscoelastic flow. We then highlight the newly emerging approach based on shear-induced diffusion, which enables direct processing of complex samples such as untreated whole blood. Finally, we hope that an improved understanding of label-free passive sorting approaches can lead to sophisticated and useful platforms toward automation in industrial, environmental, and biomedical fields.

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Section: Sorting By Pinched Flow Fractionationmentioning

confidence: 99%

Section: Sorting By Pinched Flow Fractionationmentioning

confidence: 99%

Section: Sorting By Pinched Flow Fractionationmentioning

confidence: 99%

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Label-free microfluidic sorting of microparticles

et al. 2019

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“…These separation methods are generally categorized into passive and active methods [2,3]. Microfiltration [4], inertia‐based [5], pinched flow fractionation [6], and deterministic lateral displacement [7] are among the passive methods. Dielectrophoretic [8], acoustophoretic [9], magnetic [10], and optic forces [11] are the most popular active methods used for sorting and separation.…”

Section: Introductionmentioning

confidence: 99%

Sheath‐assisted versus sheathless dielectrophoretic particle separation

Dalili

Hoorfar

2021

Electrophoresis

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Lab‐on‐chip devices are widely being used for binary and ternary cell/particle separation applications. Among the lab‐on‐chip methods, dielectrophoresis (DEP) is a cost‐effective and label‐free method, with great capabilities for size‐based separation of cells and particles, which is mostly performed in sheath‐assisted forms. However, the elimination of the sheath flows offers advantages such as ease of operation and higher sample throughput. In this work, we present a comparison of sheath‐assisted and sheathless DEP separation of three sizes of microparticles using tilted electrodes. The sheath‐assisted design was capable of separating the 5, 10, and 15 μm particles with a separation efficiency as high as 98.0% for 15 μm particles. By adding a DEP focusing region, a sheathless DEP separator was proposed, which offered higher throughputs (up to 10 times) at the cost of lowering the separation efficiency (a reduction up to 10.3% for 15 μm) compared to the sheath‐assisted design. To enhance the separation efficiency, a combination of the DEP focusing accompanied by weak sheath flows from both sides was proposed. This design achieved the highest sample separation yield in the outlets (as high as 98.7% for 15 μm) with a sample throughput of more than 4.2 μL/min. This study provides insights into the choice of an appropriate platform for any application in which the yield, purity, throughput, and portability must be considered.

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“…The pinched flow fractionation methods have been used for the continuous size separation of particles in microchannels by taking advantage of the laminar flow regime inside them [9]. One subcategory of these methods was recently reported by Tagaki et al based on the asymmetric pinched flow fractionation (AsPFF) principle.…”

Section: Introductionmentioning

confidence: 99%

In Silico Analysis of Microfluidic Systems for the Purification of Magnetoliposomes

Torres

Aranguren

Reyes

et al. 2020

The 2nd International Online-Conference on Nanomaterials

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Magnetite nanoparticles (MNPs) have been considered for several applications in drug delivery. However, the main challenge is to assure high cell-penetration levels, especially when dealing with cargoes that show limited membrane passing. A strategy is to encapsulate the MNPs into liposomes to form magnetoliposomes (MLs) capable of fusing with membranes to achieve high delivery rates. MLs have therefore been used as carriers in the biomedical field due to their ability to release active molecules that can be used in treatments of diverse diseases. There are several techniques to produce such encapsulates, however, the main challenge is that the process often leads to an important fraction of non-encapsulated MNPs. Purification of such a fraction is challenging because of the small size difference between the particles and the MLs and the reduced magnetic responsiveness. Seeking to obtain pure MLs with potential use in the medical field, the following study presents finite element simulations using COMSOL Multiphysics of two purification methods. Accordingly, we implemented the magnetic and asymmetric pinched flow fractionation (AsPFF) separation systems to evaluate their purification efficiencies considering operation parameters such as the Flow Rate Ratio (FRR) and Total Velocity Ratio (TVR). Additionally, a mixture interaction approach was used to model the MNPs as a dispersed ferrofluid phase. This was compared with a particle tracing approach where MNPs are considered individual entities subjected to hydrodynamic forces. The results show efficiencies between 60% and 90% for both separation methods, which confirms their feasibility to improve and optimize the purification of MLs in a high throughput manner.

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Separations of spherical and disc-shaped polystyrene particles and blood components (red blood cells and platelets) using pinched flow fractionation device with a tilted sidewall and vertical focusing channels (t-PFF-v)

Cited by 14 publications

References 37 publications

Label-free microfluidic sorting of microparticles

Label-free microfluidic sorting of microparticles

Sheath‐assisted versus sheathless dielectrophoretic particle separation

In Silico Analysis of Microfluidic Systems for the Purification of Magnetoliposomes

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