Separation-Type Multiplex Polymerase Chain Reaction Chip for Detecting Male Infertility

Ha, Seung-Mo; Ju, Jin-Kyoung; Ahn, Yonghan; Hwang, Seung Young

doi:10.1143/jjap.47.5231

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…Unlike PDMS chips, which can be mass produced economically by a molding technique, glass chips that contain a microheater and sensor are expensive from a disposable chip point of view. To compensate for this weakness, we make it easy to join and separate the PDMS layer and the glass substrate as shown in a previous study . A schematic of the separable biochip is shown in Supporting Information Fig.…”

Section: Methodsmentioning

confidence: 99%

Disposable on‐chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction

et al. 2013

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This paper reports the development of a disposable, integrated biochip for DNA sample preparation and PCR. The hybrid biochip (25 × 45 mm) is composed of a disposable PDMS layer with a microchannel chamber and reusable glass substrate integrated with a microheater and thermal microsensor. Lysis, purification, and PCR can be performed sequentially on this microfluidic device. Cell lysis is achieved by heat and purification is performed by mechanical filtration. Passive check valves are integrated to enable sample preparation and PCR in a fixed sequence. Reactor temperature is needed to lysis and PCR reaction is controlled within ±1°C by PID controller of LabVIEW software. Buccal epithelial cell lysis, DNA purification, and SY158 gene PCR amplification were successfully performed on this novel chip. Our experiments confirm that the entire process, except the off‐chip gel electrophoresis, requires only approximately 1 h for completion. This disposable microfluidic chip for sample preparation and PCR can be easily united with other technologies to realize a fully integrated DNA chip.

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Section: Methodsmentioning

confidence: 99%

Disposable on‐chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction

et al. 2013

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“…A schematic of the device to be used for cell lysis and DNA purification is shown in Figure 1 device is a detachable PDMS-glass biochip. 7 A disposable sample treatment chip was fabricated with PDMS by a replica molding technique, providing an economical and mass-producible chip. 8 The PDMS chip was clamped to a reusable glass chip in which a microheater and microsensor for sample heating were fabricated.…”

Section: Methodsmentioning

confidence: 99%

An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples

Ha¹,

Cho²,

Ahn³

et al. 2011

J. Nanosci. Nanotech.

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In this paper, we describe the design and fabrication of a microfluidic device for cell lysis and DNA purification, and the results of device tests using a real sample of buccal cells. Cell lysis was thermally executed for two minutes at 80 degrees C in a serpentine type microreactor (20 microL) using an Au microheater with a microsensor. The DNA was then mixed with other residual products and purified by a new filtration process involving micropillars and 50-80 microm microbeads. The entire process of sample loading, cell lysis, DNA purification, and sample extraction was successfully completed in the microchip within five minutes. Sample preparation within the microchip was verified by performing a SY158 gene PCR analysis and gel electrophoresis on the products obtained from the chip. The new purification method enhanced DNA purity from 0.93 to 1.62 after purification.

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“…Applications that combine this microfluidic chip and a micro-heater system have been reported. Although configurations that combine both systems are simple, their intended applications are diverse, and include molecular and cellular analysis such as polymerase chain reaction (PCR), trapped glucose analysis, enzyme analysis, and DNA analysis (Ha et al, 2008;Paranjape et al, 2003;Arata et al, 2006;Deng et al, 2005;Lee et al, 2008;Cheng et al, 2013).Many applications that do not use a PDMS chip have also been reported. For example, cell manipulation using a thermo-responsive polymer and a drag release system has been reported (Takeuchi et al, 2013;Chau & Melvin, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel 2-Dimensional Micro-Heater Array Device with Regional Selective Heating

Horade

Kojima²,

Kamiyama³

et al. 2016

MER

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This paper reports the development of a micro-heater array device that can be selectively heated at an arbitrary location. To confirm the heating characteristics of this micro-heater array device, heating experiments using a thermo-responsive gel were conducted. Since a micro-heater can supply heat with a rapid-response on a micro-scale, various applications have been studied. Based on these characteristics, micro-heaters have often been used in recent research applications involving biological cells. To expand the versatility of the micro-heater, the development of micro-heater array systems that can supply heat selectively at an arbitrary location is required. In this work, to support micro-heater applications in the field of biochemistry, the design and materials of a micro-heater array device were optimized and a fabrication process was established. Furthermore, the usefulness of this device was verified using a thermo-responsive gel, and control of the temperature distribution on a glass substrate was successfully demonstrated. This micro-heater array device can be heated with regional selectivity, and each region can be controlled to an arbitrary temperature, so the device is also capable of generating temperature gradients.

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Separation-Type Multiplex Polymerase Chain Reaction Chip for Detecting Male Infertility

Cited by 7 publications

References 10 publications

Disposable on‐chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction

Disposable on‐chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction

An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples

Development of a Novel 2-Dimensional Micro-Heater Array Device with Regional Selective Heating

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