Separation Tecniques: CHROMATOGRAPHY

Abstract

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most common methods of protein purification.

Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids)

“…The principle of this technique is separation. The separation technique of active components is based on their size, shape, and charge (Coskun, 2016). At the time of analysis with chromatography, analytes are present in a solution and transferred through a solid phase that acts as a sieving material.…”

Characterization of in-vitro antioxidant, cytotoxic, thrombolytic and membrane stabilizing potential of different extracts of Cheilanthes tenuifolia and Stigmasterol isolation from n-hexane extract

“…Bioactive compounds from plant extracts can be isolated by using column chromatography, TLC and PTLC and structure of obtained compounds can be elucidated by using NMR. The factors effective on separation process using column chromatography include molecular characteristics related to adsorption (liquid-solid), partition (liquid-solid), and affinity or differences among their molecular weights, polarity [21]. 10 g of silica was placed into the Erlenmeyer flask and 100% n-hexane was added to the Erlenmeyer flask containing the silica gel.…”

Application of Some Proteome Analysis Techniques in Animal Reproduction

“…If the mobile phase is liquid, it is called "liquid chromatography (LC)" and if mobile phase is gas, it is called "gas chromatography (GC)." Apart from these, it is also classified according to the type of bedding (column or paper chromatography) and separation mechanisms (ionexchange, partition, surface adsorption, and size exclusion chromatography) [33][34][35].…”