Separation, recombination, and characterization of dissimilar subunits of the DPN-dependent isocitrate dehydrogenase from pig heart.

Ehrlich, Robert S.; Colman, Roberta F.

doi:10.1016/s0021-9258(18)32335-4

Cited by 37 publications

(23 citation statements)

References 18 publications

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“…Our results showed the abundance of 13 C glucoselabeled glycolytic intermediates including phosphoenolpyruvate, pyruvate and lactate were similar between the HET and KO retinas. For TCA cycle intermediates, 13 C glucose labeled citrate, isocitrate and aconitate increased 2~3 fold in the KO retinas, while other intermediates remained unchanged (Fig 3c).…”

Section: Idh3b Knockout Mice Have Normal Growth and Vision Without Re...supporting

confidence: 54%

“…These TCA cycle metabolites can be generated from different nutrient sources such as glucose, fatty acids and amino acids. To trace the changes of metabolites from the same nutrient source, we incubated freshly isolated retinas with 13 C glucose and analyzed 13 C glucose-labeled metabolites by GC MS as previously reported 25,26 . Our results showed the abundance of 13 C glucoselabeled glycolytic intermediates including phosphoenolpyruvate, pyruvate and lactate were similar between the HET and KO retinas.…”

Section: Idh3b Knockout Mice Have Normal Growth and Vision Without Re...mentioning

confidence: 99%

“…To trace the changes of metabolites from the same nutrient source, we incubated freshly isolated retinas with 13 C glucose and analyzed 13 C glucose-labeled metabolites by GC MS as previously reported 25,26 . Our results showed the abundance of 13 C glucoselabeled glycolytic intermediates including phosphoenolpyruvate, pyruvate and lactate were similar between the HET and KO retinas. For TCA cycle intermediates, 13 C glucose labeled citrate, isocitrate and aconitate increased 2~3 fold in the KO retinas, while other intermediates remained unchanged (Fig 3c).…”

Section: Idh3b Knockout Mice Have Normal Growth and Vision Without Re...mentioning

confidence: 99%

“…The acrosome is formed by organelles-derived (particularly Golgi) proacrosomal vesicles that traffick, fuse and anchor to the nuclear surface during spermiogenesis. Acrosome biogenesis can be divided into four phases: Golgi (steps 1-3), Cap (steps 4-7), Acrosome (steps 8-12) and Maturation (steps [13][14][15][16] phases (Fig S4a). To visualize acrosome biogenesis, we stained the acrosome, nucleus and Golgi with PNA, DAPI and GM130.…”

Section: Loss Of Idh3b Disrupts Acrosome Biogenesismentioning

confidence: 99%

“…Without β or γ subunit, the α subunit alone has almost no activity 13,14 . IDH3 subunits are encoded by IDH3A, IDH3B, and IDH3G, which express abundantly in mitochondria-rich tissues such as the heart, skeletal muscle and brain.…”

Section: Introductionmentioning

confidence: 99%

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Isocitrate dehydrogenase 3b is required for spermiogenesis but dispensable for retinal degeneration

Zhu

Huang

et al. 2022

Preprint

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Isocitrate dehydrogenase 3 (IDH3), a key enzyme in mitochondrial tricarboxylic acid (TCA) cycle, catalyzes the decarboxylation of isocitrate into α-ketoglutarate (αKG), converting NAD+ into NADH. We have found that IDH3 β subunit (IDH3B) is essential for IDH3 activity in multiple tissues. Loss of Idh3b in mice causes substantial accumulation of the isocitrate and its precursors in the TCA cycle, particularly in the testes, whereas the levels of the downstream metabolites remain unchanged or slightly increased. The Idh3b-knockout (KO) mice have normal visual function without retinal degeneration. However, the male KO mice are infertile. Loss of Idh3b causes energetic deficit and disrupts the biogenesis of acrosome and flagellum, resulting in spermiogenesis arrestment in sperm cells. Together, we demonstrate IDH3B controls its substrate levels in the TCA cycle and it is required for sperm mitochondrial metabolism and spermiogenesis, highlighting the importance of the tissue-specific function of the ubiquitous TCA cycle.

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Section: Idh3b Knockout Mice Have Normal Growth and Vision Without Re...supporting

confidence: 54%

Section: Idh3b Knockout Mice Have Normal Growth and Vision Without Re...mentioning

confidence: 99%

Section: Idh3b Knockout Mice Have Normal Growth and Vision Without Re...mentioning

confidence: 99%

Section: Loss Of Idh3b Disrupts Acrosome Biogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isocitrate dehydrogenase 3b is required for spermiogenesis but dispensable for retinal degeneration

Zhu

Huang

et al. 2022

Preprint

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Which way does the citric acid cycle turn during hypoxia? The critical role of α‐ketoglutarate dehydrogenase complex

Chinopoulos

2013

J of Neuroscience Research

110

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The citric acid cycle forms a major metabolic hub and as such it is involved in many disease states implicating energetic imbalance. In spite of the fact that it is being branded as a 'cycle', during hypoxia, when the electron transport chain does not oxidize reducing equivalents, segments of this metabolic pathway remain operational but exhibit opposing directionalities. This serves the purpose of harnessing high energy phosphates through matrix substrate-level phosphorylation in the absence of oxidative phosphorylation. In this mini-review these segments are appraised, pointing to the critical importance of the alpha-ketoglutarate dehydrogenase complex dictating their directionalities.

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Identification of the Subunits and Target Peptides of Pig Heart NAD-Specific Isocitrate Dehydrogenase Modified by the Affinity Label 8-(4-Bromo-2,3-dioxobutylthio)NAD

Huang

Kumar

Colman

1997

Archives of Biochemistry and Biophysics

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Separation, recombination, and characterization of dissimilar subunits of the DPN-dependent isocitrate dehydrogenase from pig heart.

Cited by 37 publications

References 18 publications

Isocitrate dehydrogenase 3b is required for spermiogenesis but dispensable for retinal degeneration

Isocitrate dehydrogenase 3b is required for spermiogenesis but dispensable for retinal degeneration

Which way does the citric acid cycle turn during hypoxia? The critical role of α‐ketoglutarate dehydrogenase complex

Identification of the Subunits and Target Peptides of Pig Heart NAD-Specific Isocitrate Dehydrogenase Modified by the Affinity Label 8-(4-Bromo-2,3-dioxobutylthio)NAD

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