Separation, purity testing and identification of cyanobacterial hepatotoxins with capillary electrophoresis and electrospray mass spectrometry

Sirén, Heli M. M.; Jussila, Matti; Liu, Huwei; Peltoniemi, Sanna; Sivonen, Kaarina; Riekkola, Marja‐Liisa

doi:10.1016/s0021-9673(99)00105-3

Cited by 36 publications

(19 citation statements)

References 16 publications

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“…Both CZE and MEKC, which employs a surfactant above the critical micelle concentration to separate neutral and uncharged molecules based on electromigration principle, have been employed for the determination of MC [27,[57][58][59][60][61][62][63][64]. Recently also separation of MC by CEC in RP monolithic columns formed by inorganic or organic polymer monoliths were reported, but no quantitative data were given [65].…”

Section: Ce Analysismentioning

confidence: 99%

“…In the simplest CZE system with UV detection as BGE a Tris buffer of pH 6 was employed with detection at 200 nm [57], or with 40 mM acetate buffer at pH 4.0 and with detection at 238 nm [58]. The first conditions were employed for the determination of MC in fractions from preparative HPLC with LOD reported for MC-LR as 3 ppm [57].…”

Section: Ce Analysismentioning

confidence: 99%

“…The first conditions were employed for the determination of MC in fractions from preparative HPLC with LOD reported for MC-LR as 3 ppm [57]. For the same purpose also second-mentioned conditions were suitable, showing impurity of [D-dsp 3 ]MC-LR in MC-LR samples [58]. CZE with UV detection can also be carried out with capillaries coated with 5% hexadimethrine bromide and 2% ethylene glycol using as BGE 1 M formic acid [27].…”

Section: Ce Analysismentioning

confidence: 99%

“…Especially outstanding separation of ten MC variants has been reported in MEKC mode of CE with UV detection [58]. This is the preferable method for electrophoretic determination of MC as their separation can be impeded by adsorption of analytes onto the capillary wall; hence, separation conditions in basic media with micelles should prevent such interaction.…”

Section: Ce Analysismentioning

confidence: 99%

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Chromatographic and capillary electrophoretic determination of microcystins

Trojanowicz

2010

J of Separation Science

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Microcystins are cyclic peptide hepatotoxins that are produced during blooming of cyanobacteria. In recent two decades they gained a great interest because of the related ecological and public health risks. Over 70 different analogs of microcystins have been isolated from natural blooms and laboratory cultures of cyanobacteria. Because they are inhibitors of protein phosphatases they act as tumor promoters, but on the other hand, inhibitive enzymatic and ELISA methods may be employed for their screening or quantitative determination. Much larger analytical potential for this purpose have, however high-performance separation chromatographic and electromigration techniques. Based on about 70 references from original journal papers these methods are reviewed together with presentation of their preconcentration methods and clean-up procedures for analyzing environmental samples. Some attention is also focused on increasing application of LC/MS methods, and comparison of separation techniques with immunochemical or enzymatic methods.

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Section: Ce Analysismentioning

confidence: 99%

Section: Ce Analysismentioning

confidence: 99%

Section: Ce Analysismentioning

confidence: 99%

Section: Ce Analysismentioning

confidence: 99%

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Chromatographic and capillary electrophoretic determination of microcystins

Trojanowicz

2010

J of Separation Science

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“…However, these methods suffer from either tedious sample pretreatment procedures or low sensitivity. Capillary electrophoresis has been widely used for separation and detection of microcystin variables because of its simplicity, low sample volume requirement, and high sensitivity [180,[187][188][189][190].…”

Section: Current Analytical Approaches For Microcystin Detectionmentioning

confidence: 99%