Separation of α-Keto Acid Dinitrophenylhydrazones by Paper Electrophoresis and Their Colorimetric Determination

Tauber, Henry

doi:10.1021/ac60098a032

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Steroids1

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1957

1984

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Cited by 13 publications

(2 citation statements)

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“…Hydrocortisone in a mixture of sulfuric acid and glacial acetic acid produces a yellow color with a maximum at 470 µ, whereas cortisone does not produce a color in this reagent. This provides a method which is useful for the identification and determination of hydrocortisone in substance, in formulations, and in mixture with cortisone (498). A microchemical detection of the characteristic functional groups in the steroid side chains, using a series of reagents, has been devised ( 14).…”

Section: Steroidsmentioning

confidence: 99%

Pharmaceuticals and Related Drugs

Haycock¹,

Mader²

1957

Anal. Chem.

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Section: Steroidsmentioning

confidence: 99%

Pharmaceuticals and Related Drugs

Haycock¹,

Mader²

1957

Anal. Chem.

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“…with water and 1 ml. of 0-2 % (w/v) 2,4-dinitrophenylhydrazine in 2 N-HCI was added and the subsequent estimation of the 2,4-dinitrophenylhydrazone of pyruvate was carried out as described byTauber (1955), except that diethyl ether was used in place of ethyl acetate and the final measurements were made at 440 m,u on a Unicam spectrophotometer.…”

mentioning

confidence: 99%