Separation of uroporphyrin esters I and III by paper chromatography

Falk, J. E.; Benson, Amy

doi:10.1042/bj0550101

Cited by 139 publications

(29 citation statements)

References 10 publications

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“…Free porphyrins were esterified by the methanol-sulphuric acid method (Falk, 1964). Isomers of URO were identified by the method of Falk & Benson (1953) and of COPRO by the method of Chu et al (1951), using Chromagram cellulose thin-layer plates (Eastman Organic Chemicals Division) as described by Gajdos & Gajdos-Torok (1969) and Gajdos-Torok (1968).…”

Section: E T H O D Smentioning

confidence: 99%

Uroporphyrin- and Coproporphyrin I-accumulating Mutant of Escherichia coli K12

Chartrand

Tardif

Săsárman

1979

Journal of General Microbiology

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A new type of haem-deficient mutant was isolated in Escherichia coli K12 by neomycin selection. The mutant was deficient in uroporphyrinogen I11 cosynthase activity as indicated by the accumulation of uroporphyrin I and coproporphyrin I. The mapping of the corresponding hemD gene by P1-mediated transduction showed that the new gene was located between ilv and cya, at min 83 on the chromosomal map of Escherichia coli K12.

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Section: E T H O D Smentioning

confidence: 99%

Uroporphyrin- and Coproporphyrin I-accumulating Mutant of Escherichia coli K12

Chartrand

Tardif

Săsárman

1979

Journal of General Microbiology

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“…A deep , ,-2 red, red-fluorescent, band appeared on development with chloroform which passed slowly down the column and was collected as Fraction A (Kennedy, 1953 Fraction A was, therefore, considered to be uroporphyrin. Examination of this fraction by the ascending paper chromatography methods of Chu, Green & Chu (1951) and of Falk & Benson (1953) showed that the uroporphyrin was present as isomer I.…”

Section: Methods Imentioning

confidence: 93%

Porphyrin pigments in the tectibranch molluscAkera bullataO. F. Müller

Kennedy

Vevers

1956

J. Mar. Biol. Ass.

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The sudden appearance of Akera bullata in the static water tanks of H.M. Dockyard, Devonport, provided specimens for the investigation of pigments. Morton & Holme (1955) have given an account of certain aspects of the biology of this interesting tectibranch mollusc.Fifteen fresh specimens were minced in the Waring blender, and the resulting material was treated in the following ways. Method IA portion of the minced material was extracted with absolute methanol, and the extract filtered. The green solution was intensely red-fluorescent in ultra-violet light, and was submitted to long-paper chromatography (Kennedy, 1953). The solvent phases were 2:6-lutidine (5 parts) and water (3 parts), and the chromatograms were run at 23°C in an atmosphere of ammonia.When the chromatograms were viewed by ultra-violet light, they revealed a spot, red-fluorescent, with RF value 0.95, indicating a monocarboxyl porphyrin compound such as phaeophorbide. There was another spot with RF value 1.0 (i.e. travelling with the solvent), which was red, and had a blue fluorescence. This was most probably carotenoid with fat.The main purpose of this preliminary experiment was to determine the presence or absence of unchanged chlorophyll in the animal. The fact that no red-fluorescent spot with an RF value 1.0 was found, indicated the absence of this pigment.Method 2 A portion was extracted with a mixture of methanol and concentrated sulphuric acid (19: I) and allowed to stand overnight in the ice-chest. It was then diluted with an equal volume of water, and the pigmented material was extracted with Analar chloroform, giving an extract which was intensely fluorescent when viewed in ultra-violet light. This extract was roughly dried by passing it through chloroform-soaked paper, and evaporated to dryness in vacuo. The residue was redissolved in dried Analar chloroform, and chromatographed on magnesium oxide grade III (Nicholas, 1951). A deep , ,-2

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“…The isomer type was determined by chromatography on Whatman paper no. 1 according to Falk and Benson [16], using authentic uroporphyrin I octamethyl ester and uroporphyrin 111 octamethyl ester as reference compounds.…”

Section: Determination Of Factor F 4 3 0 and Of Uroporphyrinogenmentioning

confidence: 99%

“…An absorption coefficient of 220000 M-' cm-' was used to calculate theconcentration [14]. The isomer typc was determined after conversion of uroporphyrin to the octamethyl ester by paper chromatography as described above [16].…”

Section: Determination Of Factor F 4 3 0 and Of Uroporphyrinogenmentioning

confidence: 99%

Uroporphyrinogen III, an intermediate in the biosynthesis of the nickel‐containing factor F₄₃₀ in Methanobacterium thermoautotrophicum

Gilles

Thauer

1983

European Journal of Biochemistry

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Factor F,,, is a nickel-containing porphinoid present in methanogenic bacteria. The synthesis of this nickel tetrapyrrole from 5-aminolevulinic acid was studied in Mrthanobuctcrium thermoautotrophicum. This anaerobic archaebacterium was found to accumulate [14C]uroporphyrinogeii 111 (1.8 pmol/g) when growing on nickel-free medium (< 50 nmol/l) supplemented with 2 mM 5-amin0-['~C]Ievulinic acid. The accumulated urophorphyrinogen I11 was quantitatively converted to factor F,,, when the cells were incubated in aminolevulinate-free medium with 5 pM NiCl,. The newly synthesized factor had the same specific radioactivity as the precursor uroporphyrinogen 111. These findings indicate that the nickelporphinoid is biosynthetically derived from uroporphyrinogen 111. The presence and some properties of the enzymes catalyzing the synthesis of uroporphyrinogen I11 from 5-aminolevulinic acid in M . thermoautotrophicum are also described.

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Separation of uroporphyrin esters I and III by paper chromatography

Cited by 139 publications

References 10 publications

Uroporphyrin- and Coproporphyrin I-accumulating Mutant of Escherichia coli K12

Uroporphyrin- and Coproporphyrin I-accumulating Mutant of Escherichia coli K12

Porphyrin pigments in the tectibranch molluscAkera bullataO. F. Müller

Uroporphyrinogen III, an intermediate in the biosynthesis of the nickel‐containing factor F₄₃₀ in Methanobacterium thermoautotrophicum

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Separation of uroporphyrin esters I and III by paper chromatography

Cited by 139 publications

References 10 publications

Uroporphyrin- and Coproporphyrin I-accumulating Mutant of Escherichia coli K12

Uroporphyrin- and Coproporphyrin I-accumulating Mutant of Escherichia coli K12

Porphyrin pigments in the tectibranch molluscAkera bullataO. F. Müller

Uroporphyrinogen III, an intermediate in the biosynthesis of the nickel‐containing factor F430 in Methanobacterium thermoautotrophicum

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Uroporphyrinogen III, an intermediate in the biosynthesis of the nickel‐containing factor F₄₃₀ in Methanobacterium thermoautotrophicum