Separation of Urinary Coproporphyrin Isormers I and III by Thin-Layer Chromatography Studies in Healthy Subjects and Patients with Myocardial Infarction

Koskelo, Pentti; Toivonen, Ilkka

doi:10.3109/00365516609103918

Cited by 26 publications

(5 citation statements)

References 11 publications

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“…The normal pattern of coproporphyrin excretion in human bile and urine has recently been the subject of several studies (1,2,7,8). It has been shown that approximately 60% of the normal daily coproporphyrin excretion is found in bile with a I to III ratio of 2.5:1.…”

Section: Discussionmentioning

confidence: 99%

“…A discrimination between these two natural isomers appears to be made in their excretion, since they are found in unequal amounts in the urine and bile of normal man: the type III isomer predominates over the type I isomer in urine (1)(2)(3)(4)(5)(6), whereas the reverse is true in bile (7,8). In liver disease and in acquired or genetic disturbances characterized by reduced hepatic excretory function, there is an increase in the total uriReceived for publication 10 May 1972 and in revised form 14 July 1972. nary excretion of coproporphyrins (3)(4)(5)(6)9), but with a concomitant shift in the isomer ratio towards a predonminance of the type I compound.…”

Section: Introductionmentioning

confidence: 99%

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Coproporphyrin I and III excretion in bile and urine

Kaplowitz¹,

Javitt²,

Kappas³

1972

J. Clin. Invest.

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A B S T R A C T The excretion of coproporphyrin isomers I and III was studied in the rat. Both isomers were found to bind equally to rat plasma and liver cytosol in vitro and to disappear from plasma at equal rates after single injections in vivo. During equimolar infusions of isomers into bile fistula animals, both the I and III isomers were excreted in bile in a concentration ratio of 2: 1, respectively. Pretreatment of animals with ethinylestradiol or simultaneous infusions of phenoldibromophthalein disulfonate caused a reduction in total hepatic excretion with no change in the 2: 1 ratio in bile. As hepatic excretion fell, excretion of both isomers in urine rose, with an increase in the proportion of the I isomer. The findings mimic those reported to occur in man and can be explained by inhibition of a common carrier which requires a stereospecific configuration that statistically favors the hepatic transport of the symmetrical coproporphyrin I isomer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Coproporphyrin I and III excretion in bile and urine

Kaplowitz¹,

Javitt²,

Kappas³

1972

J. Clin. Invest.

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“…Although the porphyrins, copro II and copro IV have not generally been accepted as occurring in biological material, the presence of a por phyrin similar to isomer II in human urine was reported by Eriksen [4] in 1953 and confirmed by Koskelo and Toivonen [5] and Aziz et al [6].…”

Section: During the 2nd International Conference In Cape Town In 1970mentioning

confidence: 74%

Thin-Layer Chromatographie Behaviour of the Coproporphyrin Isomers in Relation to Porphyrin P(1)

Wg¹,

Darocha²,

Eales³

1974

Enzyme

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“…Several studies documented large quantities of porphyrins in meconium of different species (6); but few data are available about CP excretion in infants and in children (7,8). Moreover, several aspects of the metabolism of newborn babies remain obscure (9), particularly porphyrin metabolism itself, which involves erythroid tissue, the liver, and the biliary and urinary systems.…”

Section: Discussionmentioning

confidence: 99%

Coproporphyrin Excretion in Healthy Newborn Babies

Rocchi,

Balli,

Gibertini

et al. 1984

J. pediatr. gastroenterol. nutr.

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SummaryThe urinary and fecal coproporphyrins (CP) undergo significant changes in cholestatic diseases of both adults and infants and their determination may provide a diagnostic tool. Little is known about CP excretion in the first days of life. The authors have studied the daily urinary and fecal excretion of CP as well as the I and III isomer distribution in 10 healthy newborn babies from 1 to 10 days old. CP were determined by the solvent partition method and the isomer distribution by thin‐layer chromatographic technique. Preliminary studies on urinary porphyrin pattern were performed using a personal high‐performance liquid chromatographic method. CP excretion was almost 10 times higher on the 1st day than on the 10th, when expressed by adult standards. The isomer I accounted for almost 80% of the total amount on the first days, whereas at the end of the study, both the CP total amount and isomer distribution overlapped the infant and adult pattern. The authors propose a personal interpretation based on a possible transient enzymatic defect in the metabolic chain of heme synthesis.

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Separation of Urinary Coproporphyrin Isormers I and III by Thin-Layer Chromatography Studies in Healthy Subjects and Patients with Myocardial Infarction

Cited by 26 publications

References 11 publications

Coproporphyrin I and III excretion in bile and urine

Coproporphyrin I and III excretion in bile and urine

Thin-Layer Chromatographie Behaviour of the Coproporphyrin Isomers in Relation to Porphyrin P(1)

Coproporphyrin Excretion in Healthy Newborn Babies

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