Separation of Tricomponent Protein Mixtures with Triblock Nanorods

Abstract

Two-component triblock magnetic nanorods with gold end blocks and nickel interior blocks have been synthesized and used as affinity templates for the simultaneous and efficient separation of a three component protein mixture. The gold blocks were selectively functionalized with 11-amino-1-undecanethiol, and then glutaraldehyde was used to covalently attach nitrostreptavidin to them. His-tagged proteins bind to the nickel block and biotin-tagged proteins bind to the functionalized gold ends, allowing one to separate a mixture of three proteins with a single material. Each surface bound protein can be released selectively using imidazole for the Histagged protein and biotin for the biotinylated protein.

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

“…In recent years, developments in the field of diagnostics and biosensor technologies have been aided by the use of nanomaterials [1][2][3][4][5] . Synthesis and fabrication methods for nanoparticles in solution have evolved to the extent that particle size, shape, and composition can be easily modified.…”

Shape matters: synthesis and biomedical applications of high aspect ratio magnetic nanomaterials

“…13b). 45 The separation efficiency of His-tagged and biotin-tagged proteins was higher than 90%, based on fluorescence measurements. Similarly, Au-Ni-Au nanorods functionalized with poly-His were employed to separate a mixture of fluorescently labelled anti-human immunoglobulin G (IgG) and anti-poly-His IgG (Fig.…”

Facile synthesis of magnetic core-mesoporous shell structured sub-microspheres decorated with NiO nanoparticles for magnetic recyclable separation of proteins

“…Magnetic separation and purification is a convenient method for the selective and reliable capture of specific proteins. Recently, several novel magnetic separation systems based on magnetic nanomaterials have been reported in an effort to overcome the limitations of the Ni-NTA system, such as Au-Ni-Au triblock nanorods [31][32], Ni/NiO core-shell nanoparticles [33] and Fe 3 O 4 -mSiO 2 -NiO microspheres [34]. However, there are still some disadvantages in these systems, such as ununiform morphology, complicated synthetic process, surfactant employment, the generation of certain intermediates, as well as low separation efficiency and poor recyclability.…”